EMAGE mouse gene expression spatial database

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EMAGE:20311

Ndel1 nuclear distribution gene E-like homolog 1 (A. nidulans) ( MGI:1932915)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012621. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012621_01	euxassay_012621_02	euxassay_012621_03	euxassay_012621_04

euxassay_012621_05	euxassay_012621_06	euxassay_012621_07	euxassay_012621_08	euxassay_012621_09

euxassay_012621_10	euxassay_012621_11	euxassay_012621_12	euxassay_012621_13	euxassay_012621_14

euxassay_012621_15	euxassay_012621_16	euxassay_012621_17	euxassay_012621_18	euxassay_012621_19

euxassay_012621_20	euxassay_012621_21	euxassay_012621_22	euxassay_012621_23	euxassay_012621_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20311_wholemount_strong.wlz

20311_wholemount_moderate.wlz

20311_wholemount_weak.wlz

20311_wholemount_possible.wlz

20311_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20311_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

ventral grey horn

weak

regional

weak expression: see section 11 12 13 14 15 16

dorsal root ganglion

moderate

spotted

moderate expression: see section 17 18 19 weak expression: see section 07 08 09 10 11 12 13 14 15 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 14 15 16

vomeronasal organ

weak

regional

weak expression: see section 14

mandible

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23 weak expression: see section 10

maxilla

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23 weak expression: see section 20

clavicle

weak

regional

weak expression: see section 10 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31375
Entity Detected:	Ndel1, nuclear distribution gene E-like homolog 1 (A. nidulans) ( MGI:1932915)
Sequence:	sense strand is shown >T31375 CACGTGCTGCTGTGAACCAGATCAGCTGAGGAGCCAGGACCAGGCTTTCTTGATCATGGATGGTGAAGAT ATACCGGATTTTTCAAGTTTAAAGGAAGAAACTGCTTATTGGAAGGAACTTTCCTTGAAGTATAAGCAAA GCTTCCAGGAAGCTCGGGATGAGCTAGTTGAGTTCCAGGAAGGAAGCAGAGAGTTAGAAGCAGAGTTGGA GGCACAGTTAGTACAGGCTGAACAAAGAAATAGAGACCTGCAGGCTGATAACCAAAGACTGAAGTATGAA GTGGAGGCGTTAAAGGAGAAACTAGAGCATCAGTATGCACAGAGCTACAAGCAGGTCTCAGTGTTAGAAG ATGATTTAAGTCAGACCCGGGCCATTAAGGAGCAACTGCATAAGTATGTGAGAGAGCTGGAGCAGGCCAA TGATGACCTGGAGCGAGCAAAAAGGGCAACAATAGTTTCACTGGAAGACTTTGAACAAAGGCTAAATCAG GCCATTGAACGAAATGCATTCTTAGAAAGTGAGCTCGATGAAAAGGAATCTTTGTTGGTCTCAGTACAGA GGTTAAAGGATGAAGCCAGAGATTTAAGGCAAGAACTAGCAGTTCGGGAACGACAACAGGAAGTGACCCG CAAGTCTGCCCCCAGCTCTCCAACTCTGGACTGTGAGAAGATGGATTCTGCGGTCCAGGCTTCACTCTCC TTGCCTGCAACGCCTGTTGGGAAAGGCACAGAAAACAGTTTTCCTTCACCAAAAGCTATACCAAACGGCT TTGGAACCAGTCCACTAACTCCTTCTGCTAGGATATCAGCACTAAACATCGTGGGAGATCTCTTGCGGAA AGTAGGGGCTTTAGAATCCAAGTTAGCCGCTTGCAGGAACTTTGCAAAAGACCAAGCATCCCGCAAATCT TATGTTCCAGGGAGCGTTAACTGTGGGGTAATGAACAGCAATGGCCCAGAGTGCCCAAGGTCAGGGCGAG CAACTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5029832), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 24692. Forward Primer - name:024692_F_IRAV55-58_F09_Ndel1, sequence:CACGTGCTGCTGTGAACC; Reverse Primer - name:024692_R_SP6_IRAV55-58_F09_Ndel1, sequence:GAAAGTTGCTCGCCCTGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20310 same embryo
	EMAGE:20309 same embryo
	EMAGE:20312 same embryo
	EurExpress:euxassay_012621 same experiment
	MGI:4826627 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source