EMAGE mouse gene expression spatial database

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EMAGE:20320

Pgm2l1 phosphoglucomutase 2-like 1 ( MGI:1918224)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012530. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012530_01	euxassay_012530_02	euxassay_012530_03	euxassay_012530_04

euxassay_012530_05	euxassay_012530_06	euxassay_012530_07	euxassay_012530_08	euxassay_012530_09

euxassay_012530_10	euxassay_012530_11	euxassay_012530_12	euxassay_012530_13	euxassay_012530_14

euxassay_012530_15	euxassay_012530_16	euxassay_012530_17	euxassay_012530_18	euxassay_012530_19

euxassay_012530_20	euxassay_012530_21	euxassay_012530_22	euxassay_012530_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20320_wholemount_strong.wlz

20320_wholemount_moderate.wlz

20320_wholemount_weak.wlz

20320_wholemount_possible.wlz

20320_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20320_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 06 07 17 18 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 07 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 07 08 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 08 16 17 18

trigeminal v nerve

strong

regional

strong expression: see section 09

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 13 14 15 16 18

neural retina

strong

regional

strong expression: see section 01 02 03 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 weak expression: see section 10 11 12 13 14 15 16

renal cortex

moderate

regional

moderate expression: see section 07 08 09 10 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31383
Entity Detected:	Pgm2l1, phosphoglucomutase 2-like 1 ( MGI:1918224)
Sequence:	sense strand is shown >T31383 CAGATCCTGATGCCGACCGGCTGGCTGTGGCAGAACTTCAGGAGAATGGCCGTTGGAAAGTTTTCACAGG AAATGAGCTGGCAGCTTTGTTTGGGTGGTGGATGTTTGATTGCTGGAAGAAAAACAAACCAAACGCCGAT GTGAAGAATGTTTACATGCTAGCCACCACTGTCTCTTCTAAAATTTTAAAGGCAATTGCACTTAAAGAAG GATTTCATTTTGAAGAAACATTACCAGGCTTTAAATGGATTGGAAGTAGGATAAAAGACCTCCTAGGAAA TGGAAAAGAAGTCCTTTTTGCATTTGAAGAGTCTATTGGCTTTCTGTGTGGAACCTCCGTGTTGGACAAA GATGGAGTGAGTGCAGCTGCTGTTGTTGCTGAGATGGCCTCTTTCCTGGATACCAGGAAGGTAACACTGA TGGAGCAACTGACAAAAGTGTATGAAATATACGGTTACCATATGTCCAAAACTTCATATTTCTTGTGTTA TGATCCACCTACCATCAAAACCATATTTGAAAGGATTCGAAATTTTGAGTCTCCAAAGGAATATCCAAAA TTTTGTGGGGCTTTTGCTATTTTGCATGTACGGGACATAACCACTGGATATGATAGCAGCCAGCCAAATA AGAAATCAGTACTGCCTGTGAGTAAAAACAGCCAAATGATTACATTTACCTTCCAAAACGGCTGTGTCGC TACTCTCCGAACAAGTGGGACAGAACCGAAGATAAAGTATTATGCAGAGATGTGTGCATCGCCTGGCCAG AGTGACACTACCTTCCTGGAAGAAGAATTAAAGAAACTCATTGACGCGCTGATAGAGAATTTTCTTGAGC CCAGTAAGAACGCACTGGTCTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5004308), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 24637. Forward Primer - name:024637_F_IRAV55-58_H23_Pgm2l1, sequence:CAGATCCTGATGCCGACC; Reverse Primer - name:024637_R_SP6_IRAV55-58_H23_Pgm2l1, sequence:GCCAGACCAGTGCGTTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20322 same embryo
	EMAGE:20321 same embryo
	EMAGE:20319 same embryo
	EurExpress:euxassay_012530 same experiment
	MGI:4827176 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source