EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:20411

Ndufab1 NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1 ( MGI:1917566)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012668. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012668_01	euxassay_012668_02	euxassay_012668_03	euxassay_012668_04

euxassay_012668_05	euxassay_012668_06	euxassay_012668_07	euxassay_012668_08	euxassay_012668_09

euxassay_012668_10	euxassay_012668_11	euxassay_012668_12	euxassay_012668_13	euxassay_012668_14

euxassay_012668_15	euxassay_012668_16	euxassay_012668_17	euxassay_012668_18	euxassay_012668_19

euxassay_012668_20	euxassay_012668_21	euxassay_012668_22	euxassay_012668_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20411_wholemount_strong.wlz

20411_wholemount_moderate.wlz

20411_wholemount_weak.wlz

20411_wholemount_possible.wlz

20411_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20411_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 16 18 19

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 13 14 15 17 18 19 20 21 22

midbrain ventricular layer

weak

regional

weak expression: see section 07 08 09 10 11 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31946
Entity Detected:	Ndufab1, NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1 ( MGI:1917566)
Sequence:	sense strand is shown >T31946 GTAGCCATGGCGTCTCGTGTCCTCTGCGCCTGTGTCCGCCGCCTGCCCGCGGCCTTCGCGCCGCTGCCCC GGCTTCCCACGTTGGCCTTGGCGCGGCCGCTCAGCACCACCCTATGCCCTGAGGGAATCCGGAGGAGACC CGGGGCTCTGCAGTCCGCCTTGGCGCTCGCGCAGGTGCCTGGAACAGTCACACATTTGTGCCGCCAGTAC AGTGACGCACCCCCACTGACGTTAGACGGAATCAAGGACCGAGTTCTGTATGTCTTGAAACTCTATGATA AGATTGATCCAGAAAAGCTCTCCGTAAATTCTCATTTTATGAAGGACCTGGGCTTAGACAGTTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6774795), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61388. Forward Primer - name:061388_F_IRAW6_f02_Ndufab1, sequence:GTAGCCATGGCGTCTCGT; Reverse Primer - name:061388_R_SP6_IRAW6_f02_Ndufab1, sequence:CCAAACTGTCTAAGCCCAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20406 same embryo
	EMAGE:20410 same embryo
	EMAGE:20409 same embryo
	EMAGE:20407 same embryo
	EMAGE:20408 same embryo
	EurExpress:euxassay_012668 same experiment
	MGI:4826640 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source