EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:20466

Smarcd2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 ( MGI:1933621)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012750. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012750_01	euxassay_012750_02	euxassay_012750_03	euxassay_012750_04

euxassay_012750_05	euxassay_012750_06	euxassay_012750_07	euxassay_012750_08	euxassay_012750_09

euxassay_012750_10	euxassay_012750_11	euxassay_012750_12	euxassay_012750_13	euxassay_012750_14

euxassay_012750_15	euxassay_012750_16	euxassay_012750_17	euxassay_012750_18	euxassay_012750_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20466_wholemount_strong.wlz

20466_wholemount_moderate.wlz

20466_wholemount_weak.wlz

20466_wholemount_possible.wlz

20466_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20466_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 13 14 15

vibrissa

strong

regional

strong expression: see section 11 16 moderate expression: see section 01 02 03 17

anterior naris epithelium

strong

regional

strong expression: see section 06 07 13 14

external naris epithelium

strong

regional

strong expression: see section 06 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 04 05 06 07 08 09 10 12 13 15

vomeronasal organ

strong

regional

strong expression: see section 12

bladder

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31630
Entity Detected:	Smarcd2, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 ( MGI:1933621)
Sequence:	sense strand is shown >T31630 AGCCCCAGGACTTCATCCAGGAGTGGCTCCGTTCCCAACGCCGAGACCTCAAGATCATCACAGATGTGAT TGGAAACCCTGAGGAGGAGAGACGAGCTGCTTTCTACCACCAGCCCTGGGCTCAGGAAGCAGTGGGGAGG CACATCTTTGCCAAGGTGCAGCAGCGAAGGCAGGAACTGGAACAGGTGCTGGGAATTCGCCTGACCTAAC TGCTCAGGGATTGCCTCCTTCCTTCCTCCCCTGCCCTGGATGGAACCTGGCAAGAGCCCGTCCTCTGGGT TCTGGCTTGGGCTGCAGACATGTAGGATGGAGTGAGGTGTGTTTCCTGTCACCCTCCACTCCCCAGCTTT AGTTTCATAAATGTAGTTTTAGATCCCTCACTGCTTGGTTCCCAAAGCCTTATTACTGACCTTTTAGCGC TGGCTTTAATTGGGTTTGCAATGAGCGGCCTCAGCCCCCTGCAGGCAGGCAGGCCTGAGTAGGAGGCTGG AGGCTGTGCTTTAACTTTGTACCAGACATTTCCAAACCAAAGGCTGCTGGGTTTGCATGTTTACAGGCTC CACCCTAGGGCCAGTGCCAGAGCTGGCTTTGGGGAGCTGGGCAAGGAAGAGAAGGCCCTAGACTCTTCCT GGCCTTTCTAACCAAAGTTTTTTGCCATTCCTACAAGCCCAGTCTTGCTGCTGGTTTGTCCTTCTTTTTG GGTATTTGCACTATTTGGGGAGCAGGTTTTTCTATGTGGGAGCCACTTTTTTTGTACAGAGGTAATGGGG TTTTTCAGGGAGCCCACTTGGTGCCTCCTTCTTCCTTTCTTTTCTTAATCTATGCAAGCGGCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2647494), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13039. Forward Primer - name:013039_F_IRAV08-11_D05_Smarcd2, sequence:AGCCCCAGGACTTCATCC; Reverse Primer - name:013039_R_SP6_IRAV08-11_D05_Smarcd2, sequence:GCAGCCGCTTGCATAGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20465 same embryo
	EMAGE:20469 same embryo
	EMAGE:20464 same embryo
	EMAGE:20468 same embryo
	EMAGE:20467 same embryo
	EurExpress:euxassay_012750 same experiment
	MGI:4828324 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source