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EMAGE:20480

Igfbp4 insulin-like growth factor binding protein 4 ( MGI:96439)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20480 EMAGE:20480 EMAGE:20480 EMAGE:20480 EMAGE:20480
"Pseudo-wholemount" of euxassay_012744. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012744_01 euxassay_012744_02 euxassay_012744_03 euxassay_012744_04
EMAGE:20480 EMAGE:20480 EMAGE:20480 EMAGE:20480 EMAGE:20480
euxassay_012744_05 euxassay_012744_06 euxassay_012744_07 euxassay_012744_08 euxassay_012744_09
EMAGE:20480 EMAGE:20480 EMAGE:20480 EMAGE:20480 EMAGE:20480
euxassay_012744_10 euxassay_012744_11 euxassay_012744_12 euxassay_012744_13 euxassay_012744_14
EMAGE:20480 EMAGE:20480 EMAGE:20480 EMAGE:20480 EMAGE:20480
euxassay_012744_15 euxassay_012744_16 euxassay_012744_17 euxassay_012744_18 euxassay_012744_19
EMAGE:20480 EMAGE:20480
euxassay_012744_20 euxassay_012744_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20480Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20480_wholemount_strong.wlz
20480_wholemount_moderate.wlz
20480_wholemount_weak.wlz
20480_wholemount_possible.wlz
20480_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20480_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38225
Entity Detected:Igfbp4, insulin-like growth factor binding protein 4 ( MGI:96439)
Sequence:sense strand is shown

>T38225
GAGATCGGAGCAAGATGAAGATCGTGGGGACACCTCGGGAGGAACCCCGTCCTGTGCCCCAGGGTTCCTG
CCAGAGCGAGCTGCACCGGGCCCTGGAGCGGCTGGCCGCCTCACAGAGCCGTACCCACGAAGACCTCTTC
ATCATCCCCATTCCAAACTGTGACCGCAACGGCAACTTCCACCCCAAACAGTGTCACCCCGCCCTGGACG
GACAGCGTGGCAAGTGCTGGTGTGTGGATCGGAAGACAGGGGTGAAGCTTCCTGGGGGTTTGGAACCCAA
GGGGGAGCTGGACTGCCACCAGCTGGCTGACAGCTTCCAAGAGTGAGACCTGCCAGCAGGTGGGGGACTC
AGTGCCCCCTACTAGGCCCCTATGCTCTGGGGTTGTAGAGTTGACCTGGCTTGGAGTCTGAGTCCTGGCT
GTCTGCCTCTGGCCCTGCCCAAAATTTCCCTTTGTGTGTGTGTGCACACGCGTGCACATGCTCGTGTGTG
TGTGTTTATAGGATGTGCCCTACAGGATAAGCCTGAGCCTTCTCGTGCCCATGATGGGCTTAGATACCCC
CAAGAGGTCTGAGTGTAGCAATAGGAATTCCCAGTGTGCTTTCAAATCAATATGGAATTCATTCATTCAT
TCATTCATTCATCCACCTGAAAACACGTATTGAAAACATACTATGTGCCAGCTTTAGTTTTCAGCGCCGG
GAAGCCTCGTTGTGATTTCCTCCGATTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 146151. Forward Primer - name:146151_F_cDNA_Igfbp4, sequence:GAGATCGGAGCAAGATGAAGAT; Reverse Primer - name:146151_N_SP6_cDNA_Igfbp4, sequence:ACAATCGGAGGAAATCACAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20478 same embryo
 EMAGE:20481 same embryo
 EMAGE:20479 same embryo
 EMAGE:20477 same embryo
 EMAGE:20476 same embryo
 EurExpress:euxassay_012744 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS