EMAGE mouse gene expression spatial database

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EMAGE:20504

Slc25a15 solute carrier family 25 (mitochondrial carrier ornithine transporter), member 15 ( MGI:1342274)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012785. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012785_01	euxassay_012785_02	euxassay_012785_03	euxassay_012785_04

euxassay_012785_05	euxassay_012785_06	euxassay_012785_07	euxassay_012785_08	euxassay_012785_09

euxassay_012785_10	euxassay_012785_11	euxassay_012785_12	euxassay_012785_13	euxassay_012785_14

euxassay_012785_15	euxassay_012785_16	euxassay_012785_17	euxassay_012785_18	euxassay_012785_19

euxassay_012785_20	euxassay_012785_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20504_wholemount_strong.wlz

20504_wholemount_moderate.wlz

20504_wholemount_weak.wlz

20504_wholemount_possible.wlz

20504_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20504_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midgut

weak

regional

weak expression: see section 11 12 13 14 16

liver

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31930
Entity Detected:	Slc25a15, solute carrier family 25 (mitochondrial carrier ornithine transporter), member 15 ( MGI:1342274)
Sequence:	sense strand is shown >T31930 TCAAGGCAGGCGTTTAGTGACTGGTCAACTCAGGGTTTCAGGGAGTACAAGAACAATGTAGAATTATGTG ATTCACTGGAACTTTGAACTTGTCTTCCCTTCTTCTATTCAAAGTCTTGGATTTTTTGTGGACGGTCCTC CATTCTACACAGTATAATTTCTGCCTGTTATTGAACCCAAACAGAAAAGTCCACCCTTGGTAGAGTGAGT ATACAGGGGAACTGGTGGCCTTATACACTAGCCTTATGCACCAGCCTCGTGCAGACTGCCTTCATTTCAG GACAAATTCAGTGGAAGTCAGTGGTACACATGCAATTATGATGGCCCTGTCACCACAGGAAAACAAGACT TTGTTCTGGGTTTAATTATAAATGGTGACCAATTTTTAGGTAGATGGTTGTAGATGATTGCTTATAAATG ATCCTAGTTAGAAGGCTGTTGCTAAAAGCTGCTGAGCCTGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30276598), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61298. Forward Primer - name:061298_F_IRAW6_b02_Slc25a15, sequence:TCAAGGCAGGCGTTTAGTG; Reverse Primer - name:061298_R_SP6_IRAW6_b02_Slc25a15, sequence:GCACAGGCTCAGCAGCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20508 same embryo
	EMAGE:20505 same embryo
	EMAGE:20506 same embryo
	EMAGE:20507 same embryo
	EMAGE:20503 same embryo
	EMAGE:20509 same embryo
	EurExpress:euxassay_012785 same experiment
	MGI:4828148 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source