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EMAGE:20522

6330578E17Rik RIKEN cDNA 6330578E17 gene ( MGI:1923428)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20522 EMAGE:20522 EMAGE:20522 EMAGE:20522 EMAGE:20522
"Pseudo-wholemount" of euxassay_012758. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012758_01 euxassay_012758_02 euxassay_012758_03 euxassay_012758_04
EMAGE:20522 EMAGE:20522 EMAGE:20522 EMAGE:20522 EMAGE:20522
euxassay_012758_05 euxassay_012758_06 euxassay_012758_07 euxassay_012758_08 euxassay_012758_09
EMAGE:20522 EMAGE:20522 EMAGE:20522 EMAGE:20522 EMAGE:20522
euxassay_012758_10 euxassay_012758_11 euxassay_012758_12 euxassay_012758_13 euxassay_012758_14
EMAGE:20522 EMAGE:20522 EMAGE:20522 EMAGE:20522 EMAGE:20522
euxassay_012758_15 euxassay_012758_16 euxassay_012758_17 euxassay_012758_18 euxassay_012758_19
EMAGE:20522 EMAGE:20522
euxassay_012758_20 euxassay_012758_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20522Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20522_wholemount_strong.wlz
20522_wholemount_moderate.wlz
20522_wholemount_weak.wlz
20522_wholemount_possible.wlz
20522_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20522_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 17 18
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 06 07 16
trigeminal v ganglion
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 15 16 17 18 19 20
vagus x ganglion
strong strong
regionalstrong expression: see section 07
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 15 16
trigeminal v nerve
strong strong
regionalstrong expression: see section 08
spinal cord
strong strong
regionalstrong expression: see section 09 10 11 12 13 14
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 09 10 moderate expression: see section 15
cervical ganglion
strong strong
regionalstrong expression: see section 08 15
thoracic ganglion
strong strong
regionalstrong expression: see section 12 13
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 09 10 13 14 15 16
retina
strong strong
regionalstrong expression: see section 01 02 20 21
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 13 15 16 weak expression: see section 14
heart ventricle
strong strong
spottedstrong expression: see section 07 08 09 10 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38241
Entity Detected:6330578E17Rik, RIKEN cDNA 6330578E17 gene ( MGI:1923428)
Sequence:sense strand is shown

>T38241
CTTACCTGTAGGCCTGTGCTCTCAAAATTATTTTACACACCTTTATTCATGACCATATTTGAAATTAGTT
AAATAGAAATAATACATTTTAGTATGGCTAAGTATGTAGCTCCGTATATATTGTGTTCAGCACATTCACA
GTGCAGTACAGTACAGTCTGCCTGCCGCACTGTTCTGCATCCACCTCAGGAAATGCAAGTGTGCCCATCT
GAGGACTCCCAGCCACTGCCAGGTCGCCCGCCCTGCCCTGTCCTTGGCATACTCTCTCCTCATGGTAACC
ACCATTCTCTTTCCTGTTTCTGAATTTGAGTACCCTAAGTGCCTGGTATATGTGGACTCTTTGTTGTGTG
CATGCACTTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGTGTGTGTGTGTGTGTGTG
TGCCTCCAGCGCGGAATACCATGAAGGTTCATATCATATATCGGTTTCCTTCCTTTGTAAGGACAAATGG
TATTGTAGTATATGTTGTATGCATATTGTATGTATCTTGTTACCTACTCATCCAGCCGTGGGTACCTCGG
TTGTTCCCACCCTGGAGCTACTGACTATGCTGTGAACGTGTATATACAAATACCCCTAGGACCCGCTTTC
AGTCTTAACATACATGTAGCAGATTGTATGTCATAGGGTACTTCTGTTTTTATTAGTAGGCATTGCCATA
GTTTCCATATCAGCCACACTATTCTATGTTCTGTCCGCAGTGCACAGAGATTAGAACTGCCTATGTCCTT
GTGACTTGTATGCCATTCTAGAAGTCGTTAAGATCCACCAGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 147743. Forward Primer - name:147743_F_cDNA_6330578E17Rik, sequence:CTTACCTGTAGGCCTGTGCTCT; Reverse Primer - name:147743_N_SP6_cDNA_6330578E17Rik, sequence:ACTGGTGGATCTTAACGACTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20521 same embryo
 EMAGE:20525 same embryo
 EMAGE:20524 same embryo
 EMAGE:20523 same embryo
 EMAGE:20520 same embryo
 EurExpress:euxassay_012758 same experiment
 MGI:4822782 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS