EMAGE mouse gene expression spatial database

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EMAGE:20576

Sec14l1 SEC14-like 1 (S. cerevisiae) ( MGI:1921386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013817. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013817_01	euxassay_013817_02	euxassay_013817_03	euxassay_013817_04

euxassay_013817_05	euxassay_013817_06	euxassay_013817_07	euxassay_013817_08	euxassay_013817_09

euxassay_013817_10	euxassay_013817_11	euxassay_013817_12	euxassay_013817_13	euxassay_013817_14

euxassay_013817_15	euxassay_013817_16	euxassay_013817_17	euxassay_013817_18	euxassay_013817_19

euxassay_013817_20	euxassay_013817_21	euxassay_013817_22	euxassay_013817_23	euxassay_013817_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20576_wholemount_strong.wlz

20576_wholemount_moderate.wlz

20576_wholemount_weak.wlz

20576_wholemount_possible.wlz

20576_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20576_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

weak

regional

weak expression: see section 03 04 08 09 10 11 12 14 15 16 17 18 19 20

lung

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38212
Entity Detected:	Sec14l1, SEC14-like 1 (S. cerevisiae) ( MGI:1921386)
Sequence:	sense strand is shown >T38212 CAAAGGGGACATCGTCTTTAACATTTATCACTCCAAGAGGTCTCCCCAGCCACCCAAGAAGGACTCGCTA GGGGCTCACAGCATCACTTCCCCAGGAGGCAACAACGTGCAGCTGATAGACAAAGTCTGGCAGCTGGGCC GGGACTATAGCATGGTGGAGTCTCCTCTAATCTGCAAAGAAGGAGAGAGCGTGCAGGGGTCCCATGTGAC CAGGTGGCCAGGCTTCTACATCCTACAGTGGAAATTCCACACCATGCCAGCATGTGCTGCCACCAACCTA CCCCGAGTGGACGACGTGCTGGCCTCCCTGCAGGTCTCCTCCCACAAGTGCAAAGTCATGTACTACACAG AAGTGATCGGCTCTGAAGATTTCAGAGGCTCCATGACCAGCCTGGAATCTAGCCACAGCGGGTTCTCACA GCTCAGTGCCGCCACCACCTCCTCCAGCCAGTCGCAATCCAGCTCCATGATTTCCAGATGGCGATTTTGC TGACATCCTCCAAGAAAGCGCTTCACTCGACAGCCCACATGTGCCCAGAGATGTTTGTAGACCTATCTGA GTCGCAGCCATGTCCCAGGCTGAAGAGCTGTTCCCCTTAAGTCCATGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 142150. Forward Primer - name:142150_F_cDNA_Sec14l1, sequence:CAAAGGGGACATCGTCTTTAAC; Reverse Primer - name:142150_N_SP6_cDNA_Sec14l1, sequence:GCATGGACTTAAGGGGAACAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20573 same embryo
	EMAGE:20572 same embryo
	EMAGE:20574 same embryo
	EMAGE:20575 same embryo
	EMAGE:20571 same embryo
	EurExpress:euxassay_013817 same experiment
	MGI:4827932 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source