EMAGE mouse gene expression spatial database

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EMAGE:20689

Dync2h1 dynein cytoplasmic 2 heavy chain 1 ( MGI:107736)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014017. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014017_01	euxassay_014017_02	euxassay_014017_03	euxassay_014017_04

euxassay_014017_05	euxassay_014017_06	euxassay_014017_07	euxassay_014017_08	euxassay_014017_09

euxassay_014017_10	euxassay_014017_11	euxassay_014017_12	euxassay_014017_13	euxassay_014017_14

euxassay_014017_15	euxassay_014017_16	euxassay_014017_17	euxassay_014017_18	euxassay_014017_19

euxassay_014017_20	euxassay_014017_21	euxassay_014017_22	euxassay_014017_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20689_wholemount_strong.wlz

20689_wholemount_moderate.wlz

20689_wholemount_weak.wlz

20689_wholemount_possible.wlz

20689_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20689_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

weak

regional

weak expression: see section 11 12

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 10 11 12

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 09 weak expression: see section 13 14

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20 21 weak expression: see section 10 12 13 14 15 16

midbrain ventricular layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 14 15

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 07 08 09 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39378
Entity Detected:	Dync2h1, dynein cytoplasmic 2 heavy chain 1 ( MGI:107736)
Sequence:	sense strand is shown >T39378 TTACATCTTGTGGTGTCTTGGCTTCCAGTTCTGGAGAAGGAATTGAATACTCTTCAGCCTAAAGACAGCT TTCGTCTGTGGCTCACTGCAGAAGTTCATCCCAACTTTACCCCCATATTACTCCAATCAAGTCTGAAGAT AACATATGAGTCACCTCCAGGTTTGAAGAAGAATTTGATGCGCACTTATGAGTCTTGGACTCCTGAGCAA ATTAGCAAAAGAGATAACATACACCGAGCTCATGCTCTTTTTAGCTTAGCATGGTTTCATGCTGCCTGTC AGGAAAGAAGAAATTATATTCCACAGGGTTGGACCAAGTTTTATGAGTTTTCCTTATCAGATCTTCGGGC CGGGTACCATGTCATTGACAGGCTCTTCGATGGTACCAAAGATGTACAGTGGGAATTTGTACACGGCTTA CTTGAAAATTCTATTTATGGAGGACGTGTAGATAACTATTTTGATCTTAGAGTTCTTCAGTCCTACTTGA AGCAGTTTTTTAATTCTTCAATAATTGATGACTACCGTGCTGTCATCGAGAAGCTCCCCGAGGACGATAA GCCCAGCTTCTTCGGTCTGCCCGCCAACATTGCCCGCTCCTCTCAGCGCATGATCAGCTCTCAGGTCATC TCCCAGCTCAGGATCTTGGGAAGATCGGTCACAGCCGGTTGCAAATTTGACAGAGAAATCTGGTCTAATG AACTTTCTCCAGTTCTGAATCTTTGGAAAAAGCTGAACCAGAATTCAAACCTAATTCATCAAAAAGTATC ACCACCTAATGATCGACAAGGATCTCCAATATTATCGTTCATCATTCTTGAGCAGTTCAATGCCATCCGC TTGGTACAAAGTGTTCATCAGTCGCTTGCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81693. Forward Primer - name:081693_F_cDNA_Dnchc2, sequence:TTACATCTTGTGGTGTCTTGGC; Reverse Primer - name:081693_N_SP6_cDNA_Dnchc2, sequence:CAGCAAGCGACTGATGAACAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20688 same embryo
	EMAGE:20692 same embryo
	EMAGE:20691 same embryo
	EMAGE:20690 same embryo
	EMAGE:20687 same embryo
	EurExpress:euxassay_014017 same experiment
	MGI:4824430 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source