EMAGE mouse gene expression spatial database

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EMAGE:20705

Birc2 baculoviral IAP repeat-containing 2 ( MGI:1197009)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013954. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013954_01	euxassay_013954_02	euxassay_013954_03	euxassay_013954_04

euxassay_013954_05	euxassay_013954_06	euxassay_013954_07	euxassay_013954_08	euxassay_013954_09

euxassay_013954_10	euxassay_013954_11	euxassay_013954_12	euxassay_013954_13	euxassay_013954_14

euxassay_013954_15	euxassay_013954_16	euxassay_013954_17	euxassay_013954_18	euxassay_013954_19

euxassay_013954_20	euxassay_013954_21	euxassay_013954_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20705_wholemount_strong.wlz

20705_wholemount_moderate.wlz

20705_wholemount_weak.wlz

20705_wholemount_possible.wlz

20705_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20705_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 13 14

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 13 14 15

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 13 14 15

pons ventricular layer

moderate

regional

moderate expression: see section 13 14 15

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39385
Entity Detected:	Birc2, baculoviral IAP repeat-containing 2 ( MGI:1197009)
Sequence:	sense strand is shown >T39385 ATGGTTTCCAAGGTGTGAGTTCTTGATACGGATGAAGGGTCAGGAGTTTGTTGATGAGATTCAAGCTAGA TATCCTCATCTTCTTGAGCAGCTGTTGTCCACTTCAGACACCCCAGGAGAAGAAAATGCTGACCCTACAG AGACAGTGGTGCATTTTGGCCCTGGAGAAAGTTCGAAAGATGTCGTCATGATGAGCACGCCTGTGGTTAA AGCAGCCTTGGAAATGGGCTTCAGTAGGAGCCTGGTGAGACAGACGGTTCAGCGGCAGATCCTGGCCACT GGTGAGAACTACAGGACCGTCAATGATATTGTCTCAGTACTTTTGAATGCTGAAGATGAGAGAAGAGAAG AGGAGAAGGAAAGACAGACTGAAGAGATGGCATCAGGTGACTTATCACTGATTCGGAAGAATAGAATGGC CCTCTTTCAACAGTTGACACATGTCCTTCCTATCCTGGATAATCTTCTTGAGGCCAGTGTAATTACAAAA CAGGAACATGATATTATTAGACAGAAAACACAGATACCCTTACAAGCAAGAGAGCTTATTGACACCGTTT TAGTCAAGGGAAATGCTGCAGCCAACATCTTCAAAAACTCTCTGAAGGAAATTGACTCCACGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89481. Forward Primer - name:089481_F_cDNA_Birc2, sequence:ATGGTTTCCAAGGTGTGAGTTC; Reverse Primer - name:089481_N_SP6_cDNA_Birc2, sequence:ACGTGGAGTCAATTTCCTTCAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20704 same embryo
	EMAGE:20706 same embryo
	EMAGE:20703 same embryo
	EMAGE:20702 same embryo
	EurExpress:euxassay_013954 same experiment
	MGI:4823477 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source