EMAGE mouse gene expression spatial database

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EMAGE:21042

Cnst consortin, connexin sorting protein ( MGI:2445141)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008341. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008341_01	euxassay_008341_02	euxassay_008341_03	euxassay_008341_04

euxassay_008341_05	euxassay_008341_06	euxassay_008341_07	euxassay_008341_08	euxassay_008341_09

euxassay_008341_10	euxassay_008341_11	euxassay_008341_12	euxassay_008341_13	euxassay_008341_14

euxassay_008341_15	euxassay_008341_16	euxassay_008341_17	euxassay_008341_18	euxassay_008341_19

euxassay_008341_20	euxassay_008341_21	euxassay_008341_22	euxassay_008341_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21042_wholemount_strong.wlz

21042_wholemount_moderate.wlz

21042_wholemount_weak.wlz

21042_wholemount_possible.wlz

21042_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21042_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9264
Entity Detected:	Cnst, consortin, connexin sorting protein ( MGI:2445141)
Sequence:	sense strand is shown >T9264 GACAGCTCGTCGGCCGCCATGTCAGCGCGCGGGGCTGGAAACAGACCCGGCGCCCGGCGGTAGCCCTTGC ATTGCCTCGGATGCCCAGGCATGGAAGGTCTTTGATGAGACGTTAAAACAGCTCCACAAAAGATGCGCTA ATGGATGACAGCGACCCTCCTACATACAGTCTGCAGATAGAGCCACAAGATGGATGTCATCCGGGGGACA GCGTGGAAAGGAGAGTGACACGCTTGCCTTCTGTCTCAGACGAAAATGAAAATCAGCTTGCCGGAGACGG GCCTGCGGGTCTGACCACCAGTGAAGGCGCCATGGGGAGAGCCACAGTGTCTGAACAGGACAGTCTCAAC AATAACGAAAGCTTCCCGTCCAGCTGCGAGGCAGCCCCCACGGAGAACGCAGAAAACACTCCAAGCGAAG GTCCCAAAGATGACCCACCTTCCTTGGGACAGGACCAAAAACTACCTGCAAAAAGAAGTCCAAGGGCCAA AAAAAGCTCTCCTAAGAGTGCACCTCCAGGTGATGCCGTACCTGTAATGCAGACGCAGAATGCAACGTCG CAGGCTGCTGGCGAGGAGGAGGCTGCGGGAGTGAACGCGAATGACCCCCCCAAAGCGCCAGCGCTGCAGC CACTCTTCTCGCTGATCCGAGGCGAGGTGGCACAGATGGACTCCAGGGCACTTCCCCTCTTCCTCCACCA GGTAGCTGAGACCTATTTCCAGGAAGAGGACTATGAGAAAGCAATGAAATTTATTCAGCTTGAAAGGCTG TATCATGAGCAATTGCTTGCAAATCTTTCTGCCATTCAGGAACAATGGGAAACAAAATGGAAAGCTGTCC AGCCACGTACAGTGACGCCTCTGAGAAATTCAGAAAAGGGATTTAATGGTGAAGATTTTGAACAGCTTGC TAAAATTTGCACAACTCACCAAGACCCTCTCTTGTCCAAGCTTAAGACAGCGCCTGTGGAACCGTCACCA GAGAGGAAAAGCCTTGCAAGGGCGATAATGTCTGAAGAGGCAGTGGGGACAGAAGCAGCAGCCAAAGAGC CAGAGATTGAAACCTGTCCCAGCACGGATCCAAGTGGAGACCGGCATGAGGAGGAGCCCCAGGAGAGCAG CCCCGGCTGTCATCAGATGGAGTGGCAGACAGCTTCCCCCGAGCTTCCAGGGACTGCAGGAAAGGACCAC ACGGAGGAGCTGCCCAGCAGCACCAATGCCACACTGGACCTCCACACCCAGTCCCTGGAGACAGCCGGGA GCAGGTCAGGGCCAGCTGCTGCCTCGAATGCCTGCAAAGACAGCAGCTGCGTGCCGGCGCCTCCCACAGA GGACCACTGCGGTGTGGCCAGAGACCCTAAGGTGGCTCCTCCTAGTGAATCAGTGGCAGAGCAGAAGCTC TCCACCGGCGACGACGGTGCACTGCCTGGGTTGATTTCAGAGGGTAAATATTCACAGGCACACCGAAAAG AACTCTGCCTTCCTCTGCAGGACGCATTTGAAGCGCTGCCCAGGGACCAGCCTCACAGCAGTGAGGTAGC TGAGCCGCGGCAGCCTGATGTCACGGCCAGTGATGGGAAATCAGCACAGAGTCAGGCTGGCTTGGAGACT GGTCCTGAGAGTGCACTCTGTGGTGACAGGAAAGCCTGTGACGTAAGCACACTGTGTCTGGAAGTGTGTA TGGCCCCAGAAGAAAGGCGGGACAGTGAAGACCGAGTGAGTAAGGAAACGGAAGACTATCTGCACAGCCT TTTGGAGAGATGCTTGAAGGATGCTGAAGATTCCCTGTCCTACGAGGACATCCAGGACGACGACTCTGAC CTCCTCCAAGACCTCTCTCCTGAAGAAGCGTCCTACAGTCTCCAGGAGGATCTGCCTCCTGACGAGAGCA CGCTCTCCCTTGATGATCTGGCCAAGAAGATAGAGATCGCAGAGGCCATTCCTGCAGAAGGGCTGGTGTC CATACTGAAGAAGAGGAATGACACAGTAGGCAGCCATCCTGCACAGATGCAGCAGAAGCCGGCTAAGCGC AGAGTGCGATTCCAGGAAATAGACGACAACCTGGAGCAAGATGAGGTTGGTGGTGGTTCCTGCATCCTGC TCATCTTGCTGTGCATAGCCACTGTCTTCCTCAGTGTTGGAGGAACTGCACTCTACTGCACCCTGGGCAA CATTGAGTCACCTGTTTGCACGGACTTTGCCGACAACGTGGACTTCTATTACACTAAGCTGCTGCAGGGG GTGGCAGGGCTGAAACACTGGGTCTACCTCTCCTAGCAGTAAGCGTGGCAGGCGTGACAGACGGCTGACA GACAAAGAAGATGCAGACTTCCCCGACAGCGTTTATCTCAGAAGTCATGAGACATCCCTGCTCCCTGGCA TCAAGGACCCAAGGATGAACGTTAGAAACAGCAACATTAAACAGCAGCCCAGGGAACACAGATGTAGGCA CCGAGGACAGAGAGAGGGAGCTATGGCGAGCAGGCCCCTCTCCTTGCTGCTTCCCCGTGTCATGTGGGGC TCACACTGAGGGCTAAAGGGCACTCCTGGTTTACAGTCTTGCTCCACAGCCATTGCTTCCCACCATCATG CAATAAGTTTCTTTGTCATCAGAGAGCATTATCTTATGGCTTCAGTGCCATTTTTTAGTTAATCTCGGGA ACTGTGTGATTTAAGCCTTGGGCATTACTCTAGATTCTGGTCTCCATTTGCAAAATGGTTGTGGGGGGCG GAGGCAGGGGTGGTCTGAAGCAGTGATCACAAGTGGATAGCCAGCATAGAATACAAAGATGACCTTTATT CTTTTAACTTGACGACAATGTGCAGGCACAACTCTTTGGAGAAGGCTTACCTGTGAACTAGATAAACTGT AGAGATGTTGGCCCCGAGTTGAGAACTGACAGACCTGTGTCGATGAACTTGACAGGCCCAAAGGCACAGG GACAGAACTCCCAGGTCCCAGGTGAGAGTTCACTCTGGCTTCTATGCTGCCTCAAGAGATGAGTGCCGGG CTCTGTGCGCAGCTCAAGAGAAACTTACTGAGTGTCTGTTATGTCCTGAGAGCCTCAGGAGAGCATCCTG AGTAAGGAGTGGTTGCCACTGTGCAGGTGTTTAGGGCAGGTAAACCCATACTCTGACGAATGAAGCAGAC TACCACGTGTATGCGTACTCTCAGGCCGTGCGCTTAGGTGTATGGAATGTGTTCCTCTCGTGGGCTCATT CTAGAATGCTTCATGGGCTCATGAAGCAGAAATCCAGTCATACCCTAAAGAGACAAATGTCTAGTGTTGT TTAATTCTGTCACCAGGTGGCCATTCCTCATTTGCGGTACAGTGATCTAATGTTTAGGAGGCCTTCCTTT GACTTTTTATATTCGAGGCAACATCTTTTTTTAAAAATCATGAATTATAGTTTTTTAGCAAGTGACCGTT ATGTTCTGTTTTCCTGTCGGGATCGTTTGGAAGGAAGGAGAAAACTGGCTTTGCAGGTGTTACTGGCTGG CACAGTACCTTCCTTTGTGTCTCTGCTTATTTATTTGGAGTGTGAACTTAAGGAGCTTTTTTATGAATTG AGCGTGCGCTGCTGAGCCTGCATTCCTTCATCCCACCGCTGCCTATTTAATTAGATGCTTAGTGTTTGGA TTTTATACCATTTCAGCTCCGTGTATTTTTAGACTTATTTTGAAATTGACTTTTTATTTTCACTTATAAT ACTGCGTGGCTTATCTCTTATGTCACCATAAACTGTAATATTTTCGATGGGTATAGATCAGAGTTATTTA TTTAGAAGTATACAGAATGCTGAAATTTAGATTCGTTATACCTAAGGCTGATTTTATTAGAAGATTATTT TAATGTCCTACTATAAACTTTAAGACTTTGTATTCATATCATATGTAACATAGGTCCCATCATCCTGGAA CAGTGGTTCTATAGGAGCCACGAGCGAGGTCATCTGTGCAGGCTCCTCTCGATACACAGACATTTCACCC TCATGATTTCTAGCTGCGGGGATTTGATGATCTTTGTTTCTGCCAATTTTATGTCAAGAAAAGTTCAAAT CTCCCTCCTGTTCACCTCTTTTCGGTCACTCAGAGTCCCGTGTGATCTCTGATGTTGTATTTGCCTCTCA GCCACCTTGGCCACATTTCCAAAACCCTGGGTTTCCTGGGAGAATCCAGTAAAAGAAAGGGCTGAAATGA TACCTGGCTAACCAGTGACCCAGTTGTGTATCCAAGGAGCCGGTGCTCACCACTCGGGGAACCAGAGTGA TCGGGAGCAGACTGACCTCGTGACCTAAAGTGGGTGTGAACTCTCACCTAGAAAACCTAGGGTACCTTTG AAACCTGTAATGGTCATTTATTGCATGAATTGGAGTATCCCCCTGCAGGTGAGAGGATGCTATCCTACGT ACAAGCATCAGACAACTATGCTGAAATGTCACTCCTCCTCTCATTTGAAAGTACTGACAACTATTTATAC CTAGAGTTCTGAAGAACTGTATTTGAGGGTGTCAAATCAAGCTTGTAATTTAAAATCTACACCAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6837803 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6837803 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008341 same experiment
	EMAGE:21043 same embryo
	EMAGE:21044 same embryo
	EMAGE:21045 same embryo
	EMAGE:21046 same embryo
	EMAGE:21047 same embryo
	EMAGE:6090 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source