EMAGE mouse gene expression spatial database

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EMAGE:21045

Rnf135 ring finger protein 135 ( MGI:1919206)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008359. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008359_01	euxassay_008359_02	euxassay_008359_03	euxassay_008359_04

euxassay_008359_05	euxassay_008359_06	euxassay_008359_07	euxassay_008359_08	euxassay_008359_09

euxassay_008359_10	euxassay_008359_11	euxassay_008359_12	euxassay_008359_13	euxassay_008359_14

euxassay_008359_15	euxassay_008359_16	euxassay_008359_17	euxassay_008359_18	euxassay_008359_19

euxassay_008359_20	euxassay_008359_21	euxassay_008359_22	euxassay_008359_23	euxassay_008359_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21045_wholemount_strong.wlz

21045_wholemount_moderate.wlz

21045_wholemount_weak.wlz

21045_wholemount_possible.wlz

21045_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21045_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7677
Entity Detected:	Rnf135, ring finger protein 135 ( MGI:1919206)
Sequence:	sense strand is shown >T7677 AAGAGGTAATCCGGGGTGCTTCCTGGCTGTCGCCTAATTATGGCGGCCGTTTGTTCTGGCAATGCAGTCC CTGTGTGGCTGAGCGAGGACGACCTGAGCTGCATTATCTGCCAAGGGCTACTAGACCAGCCCACCACGCT GCCCTGTGGCCACAGCTTCTGTCTCCGGTGCCTCCATGACCTGTGGGTCTCGAAGCGTGGGGCCGTGGAT GGCTGCCCTTGGGCTTGCCCCATATGTCGGAAGGGTCCCTTGACAAAGCCCAAGCTGCACAAGAACCCGC TGCTGCAAGACCTGGTGGACAAGTACCTCCAAGCGGCGCGGGAGGTCGAGGCTGGCTCGGAGCCTGAACC CGCCCCCGCGCCGCGTTCTGCGCCCCAGGTGACAGTACAGAAAAGTACCACAAACGTTATCCAGGAGCTG ACTGACATGGTGAGACAGCTTGTAGACGATGTCAAGAGCCTTCAAACGCAAAGACCCAACTTGGGATCTG GACAGGACAATGCACAAGGCACCCCACCCACGGATTCTTCCTCTGAGGGGGAACATTCCTTGGATTCTCC AAAGCTGGTGACCTTCAGTATATCTCAGAAGAAAATTCAGGAGATTCTCCATAACCTGGAAGAAATTCAG GAGAAACTGCAAGGGAGTGTCCCAGGGAGAGCGCCCCCTAGAGAGCGAGTTCAGGAAATGACATCTTCCT TGTGCCTGCTGCCTGACCAGAGGCGTCCTGCACCCAGGAAAGCTTCTCACTTGTCCCTGTGGGCCATCAG TCCAACCTTTGACCTGAGGACCCTCTCCTATAACCTGGAGGTTTCCAACAATAGCCGGAGAGTGACTGTG TCTCGTGGTGATCTGCACACCTATCACTGGAGTCCCCAGAGATTTTCAATTAGCCAGGTCTTTTGTTCTC AGGCCCTCTCTTCTGGACAGAAGTACTGGGAAGTGGACACTAGGAACTGTAGCCACTGGGCCATCGGGGT AGCTTCTTGGGGCATGAAGCGGGACGGGATGCTGGGAAGGACTATGGACTCCTGGTGTATAGAGTGGAGA GGGCCTGGCCAGTTCTCTGCTTGGGCCAAGATGAAGAAAACAGACTTGCAGTCGGACCTCCCTGAGGTTG TGGGCGTGTGGCTGGACCTGGAGTCGGGGGAGCTTGCCTTCTACGCAGTGGCCGATCACGAGAGGCTCCT GTATGAGTGTGAGGTCTCTAGCTCCTCTCCTCTGCATCCCGCCTTCTGGCTGTATGGCTTAAGTCCTGGA AACTACCTGGAAATAAAGCAGCTAAACACATGAGCTTCCCTCGGGGTTAGGACAGTGGCTTCTTAGCCTC TTTGTTTAGGGGCCACTGGAGAATTCCACTTAGGGAGACTGGATATAGTAGTGCATACCTATAACTCCAG CACTTGGGACGTGGAGGTAGGTGGATCACTGCAAGTTTGAGGCTAGCATGGTCAATAAAGATAGTTCAAG GCCAGCTTGGACTATATGATATGACTGCCCAATTTTAAAGTTATTCTTTTTGGGAGTGAGACAGGATTAA CTAAACAAGTTTGTGTTTTTTAAACCTTGGGCATTTCAGAGAAAGAGCTGGTCTGGAGTGTCTCCTCACA GTGACCTCTGAGGCCTCAAATAGCCTGCCTAATAAGAAGGACTTCGAACCATTCCCTATGGCCTCATCTG GGACTGTTGGTTTAGTAAGCCTGTGGTCAACAAGTCACACCTACATGACTGACCCCCCACCCCCAGTTAA AAGTCTAGCCCAACAGTATAGCTGGGTGAACATCTCTAGCTAGCATATGCTATATATGTCCTCACATACC TCTGCTGGAAGGACAGAGCGCTGTCTTATGACTGGTAAGAGGGAGTGCTCCTGGACCCTGCTCGTGTGCC TTTTTCTACTGTTCATTTTATTCTGTGGCCTTTCAGTGTAATAAACTGAAATCATGACTTTCAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30744704 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30744704 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008359 same experiment
	EMAGE:21042 same embryo
	EMAGE:21043 same embryo
	EMAGE:21044 same embryo
	EMAGE:21046 same embryo
	EMAGE:21047 same embryo
	EMAGE:6090 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source