EMAGE mouse gene expression spatial database

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EMAGE:21046

F2rl2 coagulation factor II (thrombin) receptor-like 2 ( MGI:1298208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008365. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008365_01	euxassay_008365_02	euxassay_008365_03	euxassay_008365_04

euxassay_008365_05	euxassay_008365_06	euxassay_008365_07	euxassay_008365_08	euxassay_008365_09

euxassay_008365_10	euxassay_008365_11	euxassay_008365_12	euxassay_008365_13	euxassay_008365_14

euxassay_008365_15	euxassay_008365_16	euxassay_008365_17	euxassay_008365_18	euxassay_008365_19

euxassay_008365_20	euxassay_008365_21	euxassay_008365_22	euxassay_008365_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21046_wholemount_strong.wlz

21046_wholemount_moderate.wlz

21046_wholemount_weak.wlz

21046_wholemount_possible.wlz

21046_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21046_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

spotted

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2

liver right lobe

strong

spotted

strong expression: see section 3 4 5 6 7 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35158
Entity Detected:	F2rl2, coagulation factor II (thrombin) receptor-like 2 ( MGI:1298208)
Sequence:	sense strand is shown >T35158 CCCGAGGACAGTATTTCAACTCTCCACGTGAATAATGCTACCATAGGATACCTGAGAAGTTCCTTAAGTA CCCAAGTGATACCTGCCATCTACATCCTGCTGTTTGTGGTTGGTGTACCAGCCAACATCGTGACCCTGTG GAAACTCTCCTTAAGGACCAAATCCATCAGTCTGGTCATCTTTCACACCAACCTGGCCATCGCAGATCTC CTTTTCTGTGTCACACTGCCATTTAAGATCGCCTACCATCTCAATGGCAACAACTGGGTATTTGGCGAGG TCACGTGCCGGATCACCACGGTTGTTTTCTACGGCAACATGTACTGCGCTATCCTGATCCTCACCTGCAT GGGCATCAACCGCTACCTGGCCACGGCTCACCCTTTCACATACCAGAAGCTGCCCAAACGCAGCTTCTCC ATGCTCATGTGTGGCATGGTGTGGGTCATGGTTTTCTTATACATGCTGCCCTTTGTCATCCTGAAGCAGG AGTACCACCTCGTCCACTCCGAGATCACCACCTGCCACGATGTCGTTGACGCGTGCGAGTCCCCATCATC CTTCCGATTCTACTACTTCGTCTCCTTAGCATTCTTTGGGTTCCTCATCCCGTTTGTGATCATCATCTTC TGTTACACGACTCTCATCCACAAACTTAAATCAAAGGATCGGATATGGCTGGGCTACATCAAGGCCGTCC TCCTCATCCTTGTGATTTTCACAATTTGCTTTGCCCCCACCAACATCATACTCGTAATCCACCATGCCAA CTACTACTACCACAATACCAACAGCTTGTACTTTATGTATCTTATTGCTCTGTGCCTGGGGAGCCTGAAT AGCTGCCTAGATCCATTCCTTTACTTTGTCATGTCGAAAGTTGTAGATCAGCTTAATCCTTAGTCAGCAA TGGCAAGACCACCTTAGAGACCAAGGAGAGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101359. Forward Primer - name:101359_F_cDNA_F2rl2, sequence:CCCGAGGACAGTATTTCAACTC; Reverse Primer - name:101359_N_SP6_cDNA_F2rl2, sequence:TCTCTCCTTGGTCTCTAAGGTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008365 same experiment
	EMAGE:21042 same embryo
	EMAGE:21043 same embryo
	EMAGE:21044 same embryo
	EMAGE:21045 same embryo
	EMAGE:21047 same embryo
	EMAGE:6090 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source