EMAGE mouse gene expression spatial database

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EMAGE:21047

Gabra2 gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2 ( MGI:95614)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008366. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008366_01	euxassay_008366_02	euxassay_008366_03	euxassay_008366_04

euxassay_008366_05	euxassay_008366_06	euxassay_008366_07	euxassay_008366_08	euxassay_008366_09

euxassay_008366_10	euxassay_008366_11	euxassay_008366_12	euxassay_008366_13	euxassay_008366_14

euxassay_008366_15	euxassay_008366_16	euxassay_008366_17	euxassay_008366_18	euxassay_008366_19

euxassay_008366_20	euxassay_008366_21	euxassay_008366_22	euxassay_008366_23	euxassay_008366_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21047_wholemount_strong.wlz

21047_wholemount_moderate.wlz

21047_wholemount_weak.wlz

21047_wholemount_possible.wlz

21047_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21047_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 9 weak expression: see section 7 8 0 5 6 7 8

diencephalon lateral wall marginal layer

moderate

regional

moderate expression: see section 9 weak expression: see section 7 8 0 5 6

telencephalon marginal layer

strong

regional

strong expression: see section 4 7 8 9 0 1 moderate expression: see section 3 5 6 7 2 weak expression: see section 1 8 9 0 4 5 6 3 4

olfactory cortex marginal layer

strong

regional

strong expression: see section 7 weak expression: see section 9 0 1 2 4 5 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35163
Entity Detected:	Gabra2, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2 ( MGI:95614)
Sequence:	sense strand is shown >T35163 AGACTTGGTTACTTTTGGGCTGTATAGCAATGTTATACTTGGTTTTGGTTTTGGTTTTTGCTTTGTACAG TCTGACTAATAACTGCTAATTTATGACCCACTATGTATAGTATGTATATATATATAATGGTAGAGCTTAC AGTAGACCTCTAAAGGAGACCTGCATTTGCTAACTCATGGAAATACAGGCAGAAAACATCCCGTGCCAAA AGAGCCATTGCCTTTTTAACACATTTACCTAGGAACTAGTTTAAAGTGGGTTTCAGATCCCATTATTTAT TTCTTAATGTTCTAATTATAATGAAAGTCGGGATCCCATGTCACACTTTGGGAAACCTTTGATTTAGATA TTACATAGAAAAATATTTCTACTGTTGCCTAAGGATGATCAGAGATTAATATTGCCACTCAAATATATGA AACAGCCAAACCTCTGGCCTGGTTGCTTTAGGAGTGTTTAGAACCCCTGCTAGTGTCATTGGAAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94004. Forward Primer - name:094004_F_cDNA_Gabra2, sequence:AGACTTGGTTACTTTTGGGCTG; Reverse Primer - name:094004_N_SP6_cDNA_Gabra2, sequence:GCTTCCAATGACACTAGCAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008366 same experiment
	EMAGE:21042 same embryo
	EMAGE:21043 same embryo
	EMAGE:21044 same embryo
	EMAGE:21045 same embryo
	EMAGE:21046 same embryo
	EMAGE:6090 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source