EMAGE mouse gene expression spatial database

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EMAGE:21061

Kit kit oncogene ( MGI:96677)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008355. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008355_01	euxassay_008355_02	euxassay_008355_03	euxassay_008355_04

euxassay_008355_05	euxassay_008355_06	euxassay_008355_07	euxassay_008355_08	euxassay_008355_09

euxassay_008355_10	euxassay_008355_11	euxassay_008355_12	euxassay_008355_13	euxassay_008355_14

euxassay_008355_15	euxassay_008355_16	euxassay_008355_17	euxassay_008355_18	euxassay_008355_19

euxassay_008355_20	euxassay_008355_21	euxassay_008355_22	euxassay_008355_23	euxassay_008355_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21061_wholemount_strong.wlz

21061_wholemount_moderate.wlz

21061_wholemount_weak.wlz

21061_wholemount_possible.wlz

21061_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21061_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9204
Entity Detected:	Kit, kit oncogene ( MGI:96677)
Sequence:	sense strand is shown >T9204 AGAGAGAGGAGCTCAGAGTCTAGCGCAGCCACCGCGATGAGAGGCGCTCGCGGCGCCTGGGATCTGCTCT GCGTCCTGTTGGTCCTGCTCCGTGGCCAGACAGCCACGTCTCAGCCATCTGCAAGTCCAGGGGAGCCGTC TCCGCCATCCATCCATCCAGCACAATCAGAGTTAATAGTTGAAGCTGGCGACACCCTCAGCCTGACGTGC ATTGATCCCGACTTTGTCAGATGGACTTTCAAGACCTATTTCAATGAAATGGTTGAGAATAAAAAAAATG AATGGATCCAGGAAAAAGCCGAGGCCACTCGCACGGGCACATACACGTGCAGCAACAGCAATGGCCTCAC GAGTTCTATTTACGTGTTTGTTAGAGATCCTGCCAAACTTTTCCTGGTTGGCCTTCCCTTGTTTGGCAAA GAAGACAGCGACGCGCTGGTCCGCTGCCCTCTGACAGACCCACAGGTGTCCAATTATTCCCTCATCGAGT GTGATGGGAAATCTCTCCCCACGGACCTGACGTTTGTCCCAAACCCCAAGGCTGGCATCACCATCAAAAA CGTGAAGCGCGCCTACCACCGGCTCTGTGTCCGCTGTGCTGCTCAGCGTGACGGTACATGGCTGCATTCT GACAAATTCACCCTCAAAGTGCGGGCAGCCATCAAGGCTATCCCTGTTGTGTCTGTGCCTGAAACAAGTC ACCTCCTTAAGAAAGGGGACACATTTACGGTGGTGTGCACCATAAAAGATGTGTCTACATCCGTGAACTC CATGTGGCTAAAGATGAACCCTCAGCCTCAGCACATAGCCCAGGTAAAGCACAATAGCTGGCACCGGGGT GACTTCAATTATGAACGCCAGGAGACGCTGACTATCAGCTCGGCAAGAGTTGACGATTCTGGAGTGTTCA TGTGTTATGCCAATAATACTTTTGGATCAGCAAATGTCACAACAACCTTGAAAGTAGTAGAAAAAGGATT CATCAACATCTCCCCTGTGAAGAACACTACAGTATTTGTAACCGATGGAGAAAACGTAGATTTGGTTGTT GAATACGAGGCCTACCCCAAACCCGAGCACCAGCAGTGGATATATATGAACAGGACCTCGGCTAACAAAG GGAAGGATTATGTCAAATCTGATAACAAAAGCAACATCAGATATGTGAACCAACTTCGCCTGACCAGATT AAAAGGCACAGAAGGAGGCACTTATACCTTTCTGGTGTCCAACTCTGATGCCAGTGCTTCCGTGACATTC AACGTTTACGTGAACACAAAACCAGAAATCCTGACGTACGACAGGCTCATAAATGGCATGCTCCAGTGTG TGGCAGAGGGATTCCCGGAGCCCACAATAGATTGGTATTTTTGTACAGGAGCAGAGCAAAGGTGTACCAC TCCTGTCTCACCAGTGGACGTACAGGTCCAGAATGTATCTGTGTCACCATTTGGAAAACTGGTGGTTCAG AGTTCCATAGACTCCAGCGTCTTCCGGCACAACGGCACGGTGGAGTGTAAGGCCTCCAACGATGTGGGCA AGAGTTCCGCCTTCTTTAACTTTGCATTTAAAGAGCAAATCCAGGCCCACACTCTGTTCACGCCGCTGCT CATTGGCTTTGTGGTTGCAGCTGGCGCGATGGGGATCATTGTGATGGTGCTCACCTACAAATATTTTCAG AAACCCATGTATGAAGTACAATGGAAGGTTGTCGAGGAGATAAATGGAAACAATTATGTTTACATAGACC CGACGCAACTTCCTTATGATCACAAATGGGAGTTTCCCAGAAACAGGCTGAGTTTTGGAAAGACATTGGG AGCTGGTGCCTTCGGGAAGGTCGTTGAGGCCACTGCATATGGCTTGATTAAGTCGGATGCTGCCATGACA GTTGCCGTGAAGATGCTCAAACCAAGTGCCCATTTAACAGAAAGAGAGGCCCTAATGTCGGAACTGAAGG TCCTGAGCTACCTGGGCAATCACATGAATATTGTGAACCTGCTTGGCGCATGCACGGTGGGAGGGCCCAC CCTGGTCATTACAGAATATTGTTGCTATGGTGATCTTTTGAATTTTTTGAGAAGGAAGCGTGACTCGTTT ATTTTCTCAAAGCAAGAAGAGCAGGCAGAAGCGGCACTTTATAAGAACCTTCTGCACTCAACGGAGCCTT CCTGTGACAGTTCAAATGAATATATGGACATGAAGCCTGGCGTTTCCTACGTGGTGCCAACCAAGACAGA CAAGAGGAGATCCGCAAGAATAGACTCGTACATAGAAAGAGACGTGACTCCTGCCATCATGGAAGATGAC GAGCTGGCTCTGGACCTGGATGATTTGCTGAGCTTCTCCTACCAGGTGGCCAAGGGCATGGCGTTCCTCG CCTCCAAGAATTGTATTCACAGAGATTTGGCAGCCAGGAATATCCTCCTCACTCACGGGCGGATCACAAA GATTTGCGATTTCGGGCTAGCCAGAGACATCAGGAATGATTCGAATTACGTGGTCAAAGGAAATGCACGA CTGCCCGTGAAGTGGATGGCACCAGAGAGCATTTTCAGCTGCGTGTACACATTTGAAAGTGATGTCTGGT CCTATGGGATTTTCCTCTGGGAGCTCTTCTCCTTAGGAAGCAGCCCCTACCCAGGGATGCCGGTCGACTC CAAGTTCTACAAGATGATCAAGGAAGGCTTCCGGATGGTCAGCCCGGAGCACGCGCCTGCCGAAATGTAT GACGTCATGAAGACTTGCTGGGACGCTGACCCCTTGAAAAGGCCAACATTCAAGCAGGTTGTCCAACTTA TTGAGAAGCAGATCTCGGACAGCACCAAGCACATTTACTCCAACTTGGCAAACTGCAACCCCAACCCAGA GAACCCCGTGGTGGTGGACCATTCCGTGAGGGTCAACTCGGTGGGCAGCAGCGCCTCTTCTACGCAGCCC CTGCTCGTGCACGAAGATGCCTGAGCAGAAACCCAAGTCCAACAGGCTTTGCTGCTGTCTCCGACCCCGT CCTTCTGGCTTCTGTGATGGTTACTTGGTTTCCCTTTGACTTGCATCCTATTCCAGGGTAGCGAGTTCCC CACCCCACCTCCAACCCCACTGTGATTCCGCCTTTACGAGCACACACTTTAGTGGCCGATGGCCTTTTCT TTTCTGCCATCAGCCACCGTCCCGCTGCGAAGGTCCGAACTGTATGTATATATTTTCCCAATAGCAAAGT AGCTCCTACTGTAAACAGAAGGACTCCTCCTGCTTTAGAGGAGAAGGGAAGGGCGGGGTGAAACTGGATG CCCAGAGTTCTTCCCCCAGTGCTCCCCTGAGTGTATTTGAAAAGTATGGCCAGTAGTTCACTTGAAGAAT AGATGTAGTCCCATTTGGCCCTGAGAGCCATCCTTAATGATGGGAGATATATGTAGCAAGACTAGAAAAG GAAAGCCAAGCCCTTTGTGTAGAAAGCAGACCATTCTTAGAACAGAGGGCAACGGGGCATCGGAAGTCTG GTCACGCTAAGAAGACCGAGGCTGAGAAGGAACAAGCCAGGGGAAGCGTGAACAATGATGCTCTGCTCTG GGCTGCCGCTCGGGCTTCTGTACAACTGACCTGGTTTCTCAGTACTTTGCTGTCTGGGAGTAGCATTGGA ATCAAGGCCTCCTCCCTAGTCAGCCTTTGTATATACTCATCTATACGTTGTATGCGTTCATACTTTGGAG GAGGGATTTCCCACAAGCTTTCGTTTCTGTGTACAGCCCTGGCATTAGACCTACTGTGTGTAAGAATAGA TTAAGAGCCATACATATTTGAAGGAAACAGTTAAATGTTTTTTGGTTGTGGTTGTTGTTGTTGTTGTTTT AAAGAAAAAAATGTATATGCTAAGCACAATCTTTATAAGACCTCTTAGCCAACATACTTGCTCTGTCTAC ACTTCGGAACAAGCCTTCCATGTCAGAGTGGCTTTGCAGGCAGGAGAACTGAGGCTGTTTGAAAAGGTTA CCACAGGATGGAGAAAACAGTGCAGTCCTGGTTTGGATTCTCACATAGCAGGGAGCACAAGTTAAACTCA GCCTTTTATAGGCACGTCCCGGACATCGGGCCAGTATCTATTCAAGTGTGTATGTGTGTGCATGCGTGTG TCTATGCGTGTGGGTGAGTTGTGTTGGGAAACTTGCCCTGCATCCCTGAGGGTCCTCCTTCAGGACCCAA GACGTAACAGCTTCTGTCACCGCTCCTGTCTCTCCAGTTTCCCTGCATGTCGCTCACTGTCTAGAATTTA CTCAAAGCCGCCACAGAGGCTTAGCGGAGTGAAGTGCCGAAGGACCTCTTTATTTGGAGTCCTCCTGTAT TTAACAACACTCTTATCGTAGACCCATTCATTAGACCTTATGTAATGCTGCCAATCCAGGGAAACAGATT TAAAGTGTACCCCGTAGACAGGGCCCAGAGGTTCCCTTGTCCTTGCCCTCCCCCACACCACCCATGATCA CTGTCCAACATAAAGGGTTCAGTGTGTACGTGGTCATGTGTTGTCCTTACAGGATTCAGGTATGTTGCCT TCACGGTTTTCCCCACCCCCTCCTGCCCTTTATCCTTTAGGCCGTGTGGCCATGAACCTGGAAGAAGTGA TCGTTTGCACTTGAGTGCTACACTCTTGCACCTTTCCAAAGTAAGCTGGTTTGGAGGTCCTGTTGTCATG TACGAGACTGTCACCAGTTACCGCGCTCTGTTTGAAACATGTCTTTGTATTCCTAATGACTTCAGTTAGA GTAAGGAGAATAGCTGTTAATATGGATGTCAGGTACTTAAGGGGCCACACCATTGAGAATTTTGTCTTGG ATATTCTTGAAAGTTTATATTTTTATAATTTTTTTTACATCAGATGTCAGATGTTTCTTTCAGTTGCTTG ATGTTTGGAATTATTATGTGGCTTTTTTTGTAAATATTGAAATGTAGCAATAATGTCTTTTGAATATTCC TGAGCCCATGAGTCCCTGAAAATATTTTTTATATATACAGTAACTTTATGTGTAAATAATACGCTGTGCA AGTTTAAACATGTCACGTTACATGTGGGTTTTTTCTGATATGTTGTCCAACTGTTGACAGTTCTGAAGAA TTCTAATAAAAATGTAAATATATAAATCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6830637 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6830637 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008355 same experiment
	EMAGE:21060 same embryo
	EMAGE:21062 same embryo
	EMAGE:21063 same embryo
	EMAGE:21064 same embryo
	EMAGE:21065 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source