EMAGE mouse gene expression spatial database

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EMAGE:21064

Ogt O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) ( MGI:1339639)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008358_01	euxassay_008358_02	euxassay_008358_03	euxassay_008358_04	euxassay_008358_05

euxassay_008358_06	euxassay_008358_07	euxassay_008358_08	euxassay_008358_09	euxassay_008358_10

euxassay_008358_11	euxassay_008358_12	euxassay_008358_13	euxassay_008358_14	euxassay_008358_15

euxassay_008358_16	euxassay_008358_17	euxassay_008358_18	euxassay_008358_19	euxassay_008358_20

euxassay_008358_21	euxassay_008358_22	euxassay_008358_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21064_wholemount_strong.wlz

21064_wholemount_moderate.wlz

21064_wholemount_weak.wlz

21064_wholemount_possible.wlz

21064_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21064_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9202
Entity Detected:	Ogt, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) ( MGI:1339639)
Sequence:	sense strand is shown >T9202 GTTCCTAAGATTCGAGTTCAGTATTCTGTGCCGCCCCCTGAGCCCTTCAAAACACTGATACGGCTGCTGC CTTTTTTCTGCTACTTCTAGAACCATTCTTGTCAGTAGTGGCGGCAGTAGAAGTCAATTACCCCTTGTTC TATCTCAAGAAGCTCCAGATGGCGTCTTCCGTGGGCAACGTGGCCGACAGTACAGAACCAACGAAACGTA TGCTTTCCTTCCAAGGGTTAGCTGAGTTGGCACATCGAGAATATCAGGCAGGAGATTTTGAGGCAGCTGA GAGACACTGCATGCAGCTCTGGAGACAAGAGCCTGACAATACTGGTGTTCTTTTATTACTTTCATCTATA CACTTCCAGTGTCGAAGGCTGGACAGATCTGCTCATTTTAGCACCTTGGCAATTAAACAGAATCCCCTTC TAGCAGAAGCCTATTCGAATTTGGGAAATGTGTACAAGGAAAGAGGGCAGTTGCAGGAAGCAATCGAGCA TTATCGACATGCCTTGCGGCTGAAGCCTGATTTCATTGATGGTTATATTAACCTGGCAGCAGCCTTGGTA GCAGCAGGTGACATGGAAGGAGCAGTACAAGCCTATGTCTCTGCTCTTCAGTACAATCCTGATTTGTACT GTGTTCGCAGTGACCTGGGGAACCTGCTCAAAGCCCTGGGTCGCTTGGAAGAAGCCAAGGCATGTTATTT GAAAGCAATTGAGACGCAACCAAACTTTGCAGTAGCCTGGAGTAATCTCGGCTGTGTTTTCAATGCACAA GGGGAGATTTGGCTGGCTATTCATCACTTTGAAAAGGCTGTCACCCTTGACCCAAATTTTCTGGATGCTT ATATCAATTTAGGAAATGTCTTGAAAGAGGCACGCATTTTTGACAGAGCTGTCGCAGCTTATCTTCGTGC CTTAAGTTTGAGCCCAAATCATGCGGTGGTGCACGGCAACCTGGCTTGTGTGTACTACGAGCAAGGCCTA ATAGACCTGGCCATTGATACCTACAGGAGAGCTATCGAACTGCAACCCCATTTCCCCGATGCTTACTGCA ACCTAGCAAATGCTCTCAAAGAGAAGGGCAGTGTTGCTGAAGCAGAAGATTGTTATAACACAGCTCTTCG TCTGTGTCCTACTCATGCAGACTCTTTGAATAACCTTGCCAACATCAAACGGGAACAGGGCAACATTGAA GAGGCAGTTCGCCTGTATCGCAAAGCATTAGAAGTCTTCCCAGAGTTTGCTGCTGCACATTCCAATTTAG CAAGTGTACTGCAACAGCAGGGCAAGCTGCAGGAAGCACTGATGCACTATAAAGAAGCCATACGAATTAG TCCTACATTTGCTGATGCTTATTCCAATATGGGAAACACTCTAAAGGAGATGCAGGATGTGCAGGGCGCT TTGCAGTGTTATACTCGTGCCATCCAGATTAATCCTGCCTTTGCTGATGCACACAGCAATCTGGCCTCCA TTCACAAGGATTCAGGGAATATCCCAGAAGCAATAGCTTCTTACCGCACAGCTCTGAAACTTAAGCCTGA CTTTCCTGATGCTTATTGTAACTTGGCTCATTGCCTACAGATTGTCTGTGATTGGACAGACTATGATGAG CGGATGAAGAAATTGGTTAGTATTGTAGCTGAGCAGCTAGAGAAGAATAGACTGCCTTCTGTCCATCCTC ACCATAGCATGCTGTACCCTCTTTCCCATGGCTTCAGGAAGGCTATTGCAGAGAGGCATGGGAATCTCTG CTTGGATAAGATTAATGTCCTTCATAAACCACCATATGAACATCCAAAAGACTTGAAGCTCAGTGATGGC CGATTGCGTGTAGGCTATGTGAGTTCTGACTTCGGGAATCACCCTACTTCACACCTTATGCAGTCTATTC CAGGCATGCATAATCCTGATAAGTTTGAGGTATTCTGCTATGCCTTGAGCCCGGATGATGGTACAAACTT TCGAGTGAAGGTGATGGCGGAAGCCAATCATTTCATTGATCTTTCTCAGATTCCTTGTAATGGAAAAGCA GCCGACCGCATCCACCAAGATGGAATTCACATCCTTGTGAATATGAATGGGTATACCAAGGGTGCTCGGA ATGAGCTCTTTGCTCTTAGGCCAGCTCCTATTCAGGCCATGTGGCTGGGCTACCCTGGGACTAGTGGTGC ACTGTTCATGGATTACATCATCACTGATCAGGAAACTTCCCCAGCTGAAGTTGCAGAGCAGTATTCTGAG AAACTGGCTTATATGCCCCATACTTTCTTTATTGGTGATCATGCTAATATGTTCCCTCACCTGAAGAAAA AAGCAGTCATCGATTTTAAATCCAATGGGCACATTTATGATAATCGGATAGTTCTGAATGGCATCGATCT CAAAGCATTTCTCGATAGCCTACCCGATGTGAAGATTGTCAAGATGAAATGTCCTGATGGAGGTGACAAT CCAGACAGCAGTAACACAGCTCTTAATATGCCCGTTATTCCCATGAATACGATTGCAGAAGCAGTAATTG AAATGATTAACAGAGGGCAGATTCAGATAACAATTAACGGATTCAGTATTAGCAATGGACTGGCGACTAC ACAGATTAATAATAAGGCTGCAACCGGAGAGGAAGTTCCCCGTACCATTATTGTAACCACCCGTTCCCAG TATGGGCTACCAGAAGATGCCATTGTGTACTGTAACTTTAATCAGTTATATAAAATTGACCCATCTACCC TGCAGATGTGGGCAAATATTCTGAAACGTGTGCCTAACAGCGTGCTTTGGCTGTTGCGTTTTCCAGCAGT AGGAGAACCCAATATTCAACAATATGCACAAAATATGGGCCTTCCCCAGAACCGTATCATTTTCTCACCT GTGGCTCCTAAAGAGGAGCATGTCAGGAGAGGTCAGCTGGCTGATGTCTGCCTGGATACTCCTTTGTGTA ATGGACACACCACAGGGATGGATGTTCTCTGGGCAGGAACACCCATGGTGACTATGCCAGGAGAGACTCT TGCCTCTCGAGTTGCAGCTTCTCAGCTTACTTGTCTAGGATGTCTCGAGCTCATTGCTAAAAGCAGACAG GAATATGAAGACATAGCTGTGAAACTGGGAACCGATCTAGAATACCTGAAGAAAATTCGTGGCAAAGTCT GGAAACAGAGAATATCTAGCCCTCTGTTCAACACCAAACAATACACAATGGAATTAGAGCGACTTTATCT GCAGATGTGGGAGCATTATGCAGCTGGCAACAAACCTGACCACATGATTAAGCCTGTTGAAGTCACCGAG TCAGCCTGAATAAAGACTGCGCACAGGAGAATTGCCCTATACCTGAGCCTCAACCTTCTGGGGGAAGGGA ACTAGATAACATGCTTTGTGTGTATCTGTGTAGTTCTGTGTTGCAGACGGATGATATATAATGATAATAG AATAGCACATTCAGACTTACTTCCTGCATGATAGGGAGAGACAAGAAAGAAGAAATGCTATTCCACAAGG AATCTCTTAGAGTTTTGCAGCAAACAGGTGGTGCACAGGTCTGGAAGGTCTGGTCTCCCTTGGTCTTCCA TGGGATGCTTAATGTGGAGGGGAGATAGAGATTAACCAGCCGTTTTGTGATGCCGTGGATTGATCAAGTC TTCTGATCCTTTTTTTTTCTTTATATTTTGGGTTTTGGAGCTTTTAAAAATGTTTGCTTTCAGGTATTTT TACTCATGTGAAGTGATCTTGATTCTTCTGAGGTTTTAAACTAAAATGTTGCTTCCTGTTTTAGTGTCTG AACTCTGACAGGGTGACAGGGACCTTGCTGGTGTAGTCTTTTTATAGGTTTTATAAACCACATGAGCCTA TATCAGTCATTTTAGTGTCTGACCTAATGCTTGGCACTGTCAGTGCTTTGTTTAGATGATGACTTAAGAC TTTTCAGCCTGTTTTACACACCCCAGCTCCCGTTAAATTCTCCTGTGACTGCTAACAAACCCATGTCTGC TTTCAGACATCCCAGGGTGTTTCTCTCGAACATGCCATCTGGTGCCAAATGAAAATTCTTAGGAGTGAAT ATTAATCATGAAGGTCACAGTTGTGGTACTGTTATTGATAATAATATAGGGTTTTTTTCTGCCTAAGTTT TACCTGTTTCACCAGTGTTTCAGCCCTTGACTGCCCCTCCTATGCTGCTTCCAAAAGTAATAGTGTGATA AGATTTTTACCTTCCTTTCTAAAGTTTGTTTTTTTTTTAAAGTGAGTCCTGTTCTTCCCATTTCTTTCAG CAGAAATGAAATCCCAGGTAAGTATATAAGTATTCAAATGTTTGGTTAGTAAATTACAGTTCTCTCCAGT ACCTTAAATGGTGTTCACCGCTCTGAAGAAGCATCTCTATACAGGCAGTTATTTTATTTTTAGACTGTGT TAGAATGTCTGGACTTAGCTTCAAACTCTATGGATTCGGCCTTGGTAGGGAATTGTACCACCACTTGGTT TGGACTACAAATTAAACATTGGCCCTCGCGGAGCTTGAGTTCTTCATGGTAGAAAACAGTTGCATTTCCG TAAAGGGTGGAGGGCTTGAGGCTTAAATATATGAGTATGTTTATACTGCTCTACTGCACTTAGACTCTCC ACTCACAGATAAGTCTTGTCTGTTAGTAGTGCTGTAGATTAAGTCTTTCCAAAGCACCTAGGCAGTGCAC CTATTCTGTAGTTGCAGCTATTGCCTGAATGTACCTTAGCTGACAAATTATTGATAATTAAGAACTTGAA TTTGTGATTCTTACTGCTAATTAAAACCTAAGCAAGAAGTCTCATGTAATTCTGATAGCAGAATTGCATT GTGTGAACTGTGTAACTTTAGTAATGTAAATCAAGGAACATCAGTTAAGAGATTTCCTAATTTATTTTTT AACTGAATAAATATCAGTTAAAAGATTGCCAGCATGGTTACAGGTAAACTGATTTTCAGAATTCACTGAA CTACTAAATGTGGGATTGGATAATAGACTTGGAGTGCCGCTGAGGCTGAGATCTGTGGCCATTTACATAG CACATCATTCCTCCTATAGGGATGAACTTTTTTTCCGACACAAAAAGTAGCCACTGCGGTTAAAGCTTTG CTTATTGTAACAGGCTTTTATTTCCAGGTAACCTATCTGTGGAAGACCTAATTCTGAATAGAGTAATAGA TATTACTGGAAACTGATTTGTTTTTTTTTTCCTATTATACTCTGCTTTATTGAAAAAGTAAAGCATTTAA AATTGTGCTCCAGAAATTCAGATGTTGTCTTGCGATCTTTAAACAATAAAATGGATGATTTCCCCTTAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6830845 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6830845 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008358 same experiment
	EMAGE:21060 same embryo
	EMAGE:21061 same embryo
	EMAGE:21062 same embryo
	EMAGE:21063 same embryo
	EMAGE:21065 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source