EMAGE mouse gene expression spatial database

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EMAGE:21065

Lhcgr luteinizing hormone/choriogonadotropin receptor ( MGI:96783)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008388. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008388_01	euxassay_008388_02	euxassay_008388_03	euxassay_008388_04

euxassay_008388_05	euxassay_008388_06	euxassay_008388_07	euxassay_008388_08	euxassay_008388_09

euxassay_008388_10	euxassay_008388_11	euxassay_008388_12	euxassay_008388_13	euxassay_008388_14

euxassay_008388_15	euxassay_008388_16	euxassay_008388_17	euxassay_008388_18	euxassay_008388_19

euxassay_008388_20	euxassay_008388_21	euxassay_008388_22	euxassay_008388_23	euxassay_008388_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21065_wholemount_strong.wlz

21065_wholemount_moderate.wlz

21065_wholemount_weak.wlz

21065_wholemount_possible.wlz

21065_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21065_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 3 4 moderate expression: see section 0 1 weak expression: see section 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35259
Entity Detected:	Lhcgr, luteinizing hormone/choriogonadotropin receptor ( MGI:96783)
Sequence:	sense strand is shown >T35259 CTATGCCTTCCTTAGGGTGTTGATTTGGCTAATTAATATACTAGCCATCTTTGGCAACTTGACAGTCCTC TTTGTTCTCCTGACCAGTCGTTATAAACTGACGGTGCCCCGCTTCCTCATGTGTAATCTCTCCTTTGCAG ACTTTTGCATGGGGCTCTACCTGCTGCTCATTGCCTCAGTGGACTCCCAAACAAAAGGCCAGTACTATAA CCATGCCATAGACTGGCAGACAGGGAGTGGCTGCAGTGCAGCTGGCTTCTTTACTGTGTTCGCCAGTGAA CTTTCTGTCTATACCCTTACAGTCATCACTCTGGAAAGGTGGCACACCATCACCTATGCTGTTCAGCTGG ACCAAAAGCTGAGGCTGAGACATGCCATCCCAATTATGCTCGGAGGATGGATTTTTTCTACCCTGATGGC CACATTGCCCCTTGTGGGTGTCAGCAGTTACATGAAAGTCAGCATCTGCCTCCCCATGGATGTGGAATCC ACTCTGTCACAAGTCTACATATTATCCATCTTGCTCCTCAATGCAGTGGCCTTTGTCGTCATCTGTGCTT GCTACGTTAGGATATACTTTGCAGTTCAAAATCCAGAGCTGACGGCTCCTAACAAGGACACAAAAATTGC TAAGAAGATGGCCATCCTCATCTTCACAGACTTCACATGCATGGCACCCATCTCATTCTTTGCCATCTCA GCTGCCTTCAAAGTACCCCTTATCACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99809. Forward Primer - name:099809_F_cDNA_Lhcgr, sequence:CTATGCCTTCCTTAGGGTGTTG; Reverse Primer - name:099809_N_SP6_cDNA_Lhcgr, sequence:AGTGATAAGGGGTACTTTGAAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008388 same experiment
	EMAGE:21060 same embryo
	EMAGE:21061 same embryo
	EMAGE:21062 same embryo
	EMAGE:21063 same embryo
	EMAGE:21064 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source