EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21066

1200016B10Rik RIKEN cDNA 1200016B10 gene ( MGI:1914125)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007027. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007027_01	euxassay_007027_02	euxassay_007027_03	euxassay_007027_04

euxassay_007027_05	euxassay_007027_06	euxassay_007027_07	euxassay_007027_08	euxassay_007027_09

euxassay_007027_10	euxassay_007027_11	euxassay_007027_12	euxassay_007027_13	euxassay_007027_14

euxassay_007027_15	euxassay_007027_16	euxassay_007027_17	euxassay_007027_18	euxassay_007027_19

euxassay_007027_20	euxassay_007027_21	euxassay_007027_22	euxassay_007027_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21066_wholemount_strong.wlz

21066_wholemount_moderate.wlz

21066_wholemount_weak.wlz

21066_wholemount_possible.wlz

21066_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21066_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9389
Entity Detected:	1200016B10Rik, RIKEN cDNA 1200016B10 gene ( MGI:1914125)
Sequence:	sense strand is shown >T9389 CTCTGGGAAGGCGCTTTGGGTGTCCGACTGGTCCGGGGAATCGCCACTGTCGCTGTGGCTGCTGCTGCCG CGGACTCAGACCCTGTGATGTTTCCAGTCTTTGGCTACTGTATCCTAGCACAGGATGCTGCTTTGTACTA CCAGTGCTCACAAGCTTGAGACTGTTTATGACCATAAGTTTCTAAAGATGTCTATCAAAGAATCTTGTGC AAAGGAAGAAAAGTCTCAGAAAAAACAGACTATCTCTTCACCAACTTTTAATGAAGACAAGAAAAAGGGA GAAATCTCAGCAGGTTCTACTAGTTCAGAACATGGCGTTCAGCCTGTTTCAACCAAGAAGAGAAAACTGA AAGCAGATGATACAGACAATGTATACTATAATGCAAACAGAAAAAACAGTAAAAGATTGAATGTAGAAGT TTTCATTCCTAAAAAGAGACTAAAATTCAGTTCTTCGACCCAAGCGGTATCATATTTAAATAATAATCAA ATGACTTCACACAGTTGTTCATCAAATGGAACTAAAGATACTAAAGTTAAAGATTGTAAATTGACAAATA TTGGAAGTAAGCTTAATTATGAAATTAAAAACCATAGCCGCATTAAAATTACCAAAGATATGAAATCTAA ACCTGTAGACCAGACAAAGGAAAAGAATTGGCCTTCTTTGCTTATTCAGAAGAAGATGAAAGAACTCAAG AAAGAAAAAAATAATAAAGACAGTTCTGAAGAATTGGAGAAATGCAAGAAAAATCACCTTCCTCAGAATT ATAATTTTAGCAATATGATCAAGGAATCATTTGAATCAGGAAGAAAGAAGATTAGCTTCAAAATTCCAAA AAAATCCTCTACTACCCTCCAGAAGCTTGTCGAAGAAAAGATCTTTACTATAGATTCTAGCAAGTCAAAG AGTAAGCAGGAGGAGAAGCAACACTTGCAAAGTCACCAGATGTCATTAAATCTGGCCAGGCACAAAACTG AAAACTCATTTTCAGATTCTACACATAAGCAGTCTGTTTGTGAGAGGATAAGAAAGGACCATTGTGAGCA CCAAGGAACGAGTAGTTCCGATTCTAAGGAAAACCATGCCCAGAGTTTTGAAACACCATGTTCTTCAGTG TCATCTGAGGATACCCAAGATGCAGATGAAGAGATGCAGATAGTAGAAGAGCTCCACGCTGCACGTGTCG GGAGAAGCGTGGATCTGCCTGTGGTTCCACCGTCTGGAGAGCTCATGAGCATGGAGATTGACCTGGTGGA TGAGGACGCACATCCCTCTGCTGCACACACTGCCTCAGACACGAAGCTTCTCATTGTTATGGACACAAAT GTTCTCATGAATCATCTCAAATTCGTTAAAATTTTGAAGACGACAGAAATACCAGGATTTGACACACTTG TGTTAATAATTCCTTGGGTGGTTATACAAGAGCTAGATCGTATGAAGGCAGGAAAATTACTAAAACATGT TCAGCATAAAGCTGTACCTGCAATTCATTTTATCAACAACAGCCTCAAAAGTCAAGACAGAAAGCTGTGG GGCCAGTCATTACAGCTGGCATCCCAAAAACTTTATGGACTGAGTGACGAGAACAATGATGACCGAGTAT TAAAGTGCTGTCTTCAGTACCAGCAATTATTCCCGTGTTCTCTTGTTATTCTGTGCACGGATGATAGAAA TTTAAGAACTAAAGGCCTGATAAGTGGTGTGAAGTCCCTCAGTAAAGAAGATCTGGATACAGAGATATTA AATTTAACTCTGAACACAGATTTGTGTCATCAGCCTTGTATTTCTAAGCAGCAGTTGAACGCAGAAGTAC CACCTGCGAAAGAGACTCCTAAGGAAGGAGCTGCAAATTCCGGACTGTCCATTCTACTGGAGCGGATCAT TTGTGATCTTGAAAAATGCCTTGGGACAGCTTTATCTTCAATATTAGAAACAGAAATGAAAATTGCTTTT GGAAATCTTTGGATGGAGGTGCTGTATTTGAAACCACCATGGACTCTAATAAATCTATTACAGTGCTTTA AAAAACATTGGCTGGCTGTATTTGGATTAGTTATGGAAAATAACTTGCTTGTAACTGTTGAGAGCCTATA TGAAAATCTCTGTAAAGCAAATAAGGCAGTGGATTTTACAACAGTGAAATTCTTGCTTCAGGATTCTAAA AGTTTGTTACATGCCTTCAGTACAAGGTCAAATTATGATGGAGTTCTTCCCCAGACCTTTGCTCAAGTAA ACAAACTACTGCAAGTACTCGCAGAGGTCAAGACAAAACTGAAGCCAAGTTCTTCAGAAAGCACAAGGAG TAAAGAGCAGGAAGACACTTCTTTGGTGAAGCTTCATAACCAAGATGTCAACGTTTTCCTGGATTCTAAT CTTCCCCAGGCCACCAGGCATCAGGAAATCTGGTCTGTCCTGGAGAGTGTTTGGATGTCAATATATCAGA ACAGCATCTCTGTCTTCATAAGTGACACAGTAACTTTTAACCTGGTTGCTGTGTCAATATATCAGAACAG CACAGATGTATTTCAAACACTGGACTCAAGTTCAACCCTGACAACTTCGAAAATAGCATCCTTTGAAGAA GCTTTTGTGTATCTTCAAAAGTTAATGGTGGCTGTGAAAGATATTCTTGAAGGAATTCAAAGGATTTTGG CTCCTAATAGTAATTACCAAGATGTTGAGACTCTCTATAACTTCCTAATCAAGTATGAGGTAAACAAAAA TGTCAAATTCACTGCCCGGGAACTTTATGATTGTGTTTCTCAGACTGAGTACCGGGAAAGATTAACCATT GGATGCCGCCAGCTGATTGAGATGGAATGCACCATGCAGCAGTGCAGTGCATCTGTCTATATGGAGGCCA GAAGCAGAGGGTGGTGTGAAGACATGCTCAACAACAGGCTTAAGTTTACAGCAGGAACAGCATCTCTGTC TTCATAAGTGACACAGTAACTTTTAACCTGGTTGCTTTTTTTGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6855442 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6855442 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007027 same experiment
	EMAGE:21067 same embryo
	EMAGE:21068 same embryo
	EMAGE:21069 same embryo
	EMAGE:21070 same embryo
	EMAGE:21071 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source