EMAGE mouse gene expression spatial database

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EMAGE:21067

Fam19a4 family with sequence similarity 19, member A4 ( MGI:2444563)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007030. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007030_01	euxassay_007030_02	euxassay_007030_03	euxassay_007030_04

euxassay_007030_05	euxassay_007030_06	euxassay_007030_07	euxassay_007030_08	euxassay_007030_09

euxassay_007030_10	euxassay_007030_11	euxassay_007030_12	euxassay_007030_13	euxassay_007030_14

euxassay_007030_15	euxassay_007030_16	euxassay_007030_17	euxassay_007030_18	euxassay_007030_19

euxassay_007030_20	euxassay_007030_21	euxassay_007030_22	euxassay_007030_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21067_wholemount_strong.wlz

21067_wholemount_moderate.wlz

21067_wholemount_weak.wlz

21067_wholemount_possible.wlz

21067_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21067_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 3 moderate expression: see section 7 8 9 4 5 6

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 9 0 2 3 4 5 6

rest of cerebellum mantle layer

strong

regional

strong expression: see section 7 8 9 7 8

pons mantle layer

strong

regional

strong expression: see section 8 9 0 1 2 3 4 5 6

midbrain mantle layer

strong

regional

strong expression: see section 7 8 9 0 1 2 3 4 5 6 7

glossopharyngeal ix ganglion

strong

spotted

strong expression: see section 9 moderate expression: see section 8

trigeminal v ganglion

strong

spotted

strong expression: see section 3 4 5 6 7 6 7 8 9 0 1 2 moderate expression: see section 8

vagus x ganglion

strong

spotted

strong expression: see section 9 moderate expression: see section 8

basal columns

strong

regional

strong expression: see section 2 3 6 moderate expression: see section 7

dorsal root ganglion

strong

spotted

strong expression: see section 9 0 1 2 5 6 7 8 9

inner ear

strong

spotted

strong expression: see section 2 3 4 5 6 7 8 9 6 7 8 9 0 1 2 3

lower jaw incisor

strong

regional

strong expression: see section 9 0 1 4 5

lower jaw molar

strong

regional

strong expression: see section 7 7 8

upper jaw incisor

strong

regional

strong expression: see section 9 0 4

upper jaw molar

strong

regional

strong expression: see section 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9987
Entity Detected:	Fam19a4, family with sequence similarity 19, member A4 ( MGI:2444563)
Sequence:	sense strand is shown >T9987 GCGCGCAGGGCTGTCTGCACCCTCTGGGATCTGGCTGGGACAATGTTTCTTCTTTAAGACACGGCGCTTC CCACGGTAGCTTTGGCTTCTCTTTGCAGCAGCACCGGAACTTTCCCCACTTTCGGCCGTACTAAGAGGGT GATGGGCGCAAGTCGCCAGCTGAGCATCCTTTGGTTTCAGTGCTGTGATCGGCGCTTCACTCTGGACTCC CGGATCTGACTCGGCTGCTCTTTCTAGTCTAACAGGTCCTCCAAGCCCAAGAAGTTTTGGGGGGCTGCAG AGATGATTGGTCCCAGTTACAAAGGGACATTCACGGTCACTCTCCAGGACCTTCTTGAGTAACAGTGTTT GACTGGAACCATATACTGTGAACTCTCCAAGGATGAGAGCCTGTGCTAAGTGGGTGCTGCTGTCACGCTG GCTGGTTCTGACCTATGTGTTAATGGTCTGCTGTAAGCTGATGTCCGCCTCGAGCCAGCACCTCCGGGGA CATGCAGGTCACCACCTGATCAAGCCAGGTACCTGCGAGGTGGTCGCCGTGCACCGCTGCTGCAATAAGA ACCGCATAGAGGAGCGCTCTCAGACGGTCAAATGTTCCTGCTTCCCAGGCCAGGTGGCTGGCACAACTCG GGCACAGCCTTCTTGTGTTGAAGCTGCCATTGTGATTGAGAAGTGGTGGTGCCACATGAATCCTTGCTTG GAAGGAGAGGACTGTAAAGTGCTTCCAGACTCTTCGGGTTGGTCCTGTAGCAGTGGCAATAAAGTCAAAA CCACCAAGGTGACCCGGTAGCAGACACAAAAGTGTTTTGACCCCGAGGGGCATCGGGACGCAGAGCTGTG CTGTGTGGATTACCAGACTAGTGAGAACTCACTCACCTTGGAACTTGCTCAGAAGTTCATAGCCAAAATA TGTGCAGTGTGGAAGGTGGCATTTACGTGGCTGTTCCCGTTTGTCTGCTTTGTGATCTCATCACACTGAA CTACAGCTTAGGTGGCCACTCAACTGAGGTGTTACCTGGATCTCAAAAGGAGCCTGCCTAGCACAAGAAA TGCTGGAGAGGACATCGGAGGTGCTAATGTGGATGGAGCAGCGCTGGGAGAACCTGGATCTTCTTGTTCT CCTTTTCCCAAGGAACATGGACAGAAGTACAAAAGGACCCAGGCCGATCCTCTTTTAAATGTGTCCCTTA GCCATTATACAGAAGGCATGAAAGCAGTCTAAGTGACAGAAGATTTGGCTCATATACGTGTGTTCTAGAT TAAAGAATTTGAATTATCTGGTGCAAGAAATTAACTTTATGAACCTTGGAGAATGGAAGGACTCATTGGG TTACTATGTGCACATTTGTATTTTTTACTTTTGATAATTTGACATGTTTATCTTCTTAGTTCCATTCCTA CGTTAGCAGAGATGTAGCAGAAGCTTTGTAATCCTGGTACACTGGTGGCTGAGTTCTATGGAAAGCAGAG TAGCACCAGAGGTTTACAACCAAAGCTTTCTCATGGCATATTCACAAAAGATCATGTACCTGTTGGGACT TTTATGGCTTGGGGTTAATGAGATAGGAGAATCATTTAAGACAGAGGACAGTCTCTGTTTAGAGGAAGGG TGATTTCTTAGATCTACTTTATGGGAATATTTTTATTATCTCCGAGAAGAAGTCTTTAAATGCTAACTAT TCACATGACAGTTGACGCAAGCCTAACAGAGTCCCTTCTGTACCCGTTTGTTCGGTTTCTTACTTCTTTA AATGTTAAGTAAAACCTCAGTCATCTTGCCTCCAATTTCAGTGATTTCACAGACACTGCCATGCAGAAAC ATAAGAAACAAAGAGACACTGGCCCAGGTTTGAAATGATGTTATTCATGTACACACATCTGTAATGTGGG ATTGATTGTATGTATTAAGAAATGTTGACAGTATACTTGCTGCGCCAACTGTCTGTCCTTTTGCAATTAC AAAGAAACATAGGTTCTATTATGTTGTCTACAGTGCTATCAATTATTTGATTTTCTTGCAGAAGAAGTGG GAATGGGTGCTACCTGTTTATAGGTTAAACTTTGGTTTGAACTTGGCTGACTGGGTGTCTACTGGAATAG TTATTCTCATATGACTAAGTAAAATATCACTCTGTTGATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:30504398 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30504398 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007030 same experiment
	EMAGE:21066 same embryo
	EMAGE:21068 same embryo
	EMAGE:21069 same embryo
	EMAGE:21070 same embryo
	EMAGE:21071 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source