EMAGE mouse gene expression spatial database

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EMAGE:21069

Mynn myoneurin ( MGI:1931415)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007036. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007036_01	euxassay_007036_02	euxassay_007036_03	euxassay_007036_04

euxassay_007036_05	euxassay_007036_06	euxassay_007036_07	euxassay_007036_08	euxassay_007036_09

euxassay_007036_10	euxassay_007036_11	euxassay_007036_12	euxassay_007036_13	euxassay_007036_14

euxassay_007036_15	euxassay_007036_16	euxassay_007036_17	euxassay_007036_18	euxassay_007036_19

euxassay_007036_20	euxassay_007036_21	euxassay_007036_22	euxassay_007036_23	euxassay_007036_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21069_wholemount_strong.wlz

21069_wholemount_moderate.wlz

21069_wholemount_weak.wlz

21069_wholemount_possible.wlz

21069_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21069_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9323
Entity Detected:	Mynn, myoneurin ( MGI:1931415)
Sequence:	sense strand is shown >T9323 TGCTTTCGAACATCTCATCTCTGTTGCCAGGTAAGGGCATTCTGGTAGATGAGCTTTGGTGAGCCGGTTC AAACAGAATTCTGGGGGTCCCAAGAGCTTTCGGATATGCAAAAGAGGAAGGAGTGATTGGTCTCGACTTA AGCATATCCCTCCCACTCGCCCCGGTCAAGAACCAGGGTGCACAACACAGTCATCTCCACTGGAGGGGCG CTAATGAAGACAGGATGACAGGCGAGGAAAAGAACTACAACTCCCAGCATGCAGCGCGCAGCGCGGTTCC CGGAGAGGCCGGCTCTGCACTCAAGCCCGGTCTCCATCCAGAATCTGGGCCGACAGCTGCGGGCTGACAA GGTGAGCAGCTCGCACCGGATGCGAGTGAGCAAGGAGGTGTGATCCGGAGACCTGGGCATGCCGGGAAGT CAGGGCTCTGAGACCCAGCTCGCCGGCCCTAGTAGACTTTTAGCGTCCTATCTCCGGTGTCCCCAATGGA CCACACACCCGGACGTGACGCTATCGCAGAGGAGGAAGGGAGAGGCGGAGCGTCGCAGTGACGTCCTCCC AAGATGGCGGTGAGAGAGTGAAGAAACTGTTTCCCTTGGGCGGCTACGGATCAAGGGTGAAATCCCATTC TGCTAGCAAAATGCAGTATTCACACCACTGTGAGCACCTTTTAGAGAGACTGAATAAGCAACGGGAAGCG GGATTTCTTTGCGACTGTACCGTAGTGATTGGAGAATTCCAGTTTAAGGCGCATAGGAATGTGCTTGCCT CCTTTAGTGAGTATTTTGGGGCGATCTACAGAAGCACTTCTGAGAACAATGTTTTTCTTGATCAGAGTCA GGTGAAGGCTGACGGATTTCAGAAACTGTTGGAGTTTATATATACAGGAACTTTAAATCTCGACAGTTGG AATGTTAAAGAAATCCATCAGGCTGCTGACTATCTCAAAGTAGAAGAGGTAGTAACTAAATGTAAAATAA AGATGGAGGATTTCGCTTTCATTGCTAGTCCCTCTTCTACCGAAATATCTAGTATTACTGGAAACATCGA ATTGAATCAACAGGCCTGTCTTCTTACCCTGCGAGATTATAACAATCGGGAGAAATCAGAAGTCTCCACG GACTCAGTTCAGGCAAACCCTAAACCACGGGCATTGACAAAGAAATCGTCTCAAAGTAAAAAGAAGAAGA AGGCCTTCAGTTCTCAGAAACCAGGGCAGAGCAAAGCAGTGCAGTACCCCAGTGACGTTCTAGAGAGCGC GTCTGTGGAATTATTCCTAGAGACGAGCAAGTTGTCCTCACCTGTAGTAGAGCAAATCATTCAAGGAAAT GACAGTTCAGAACTTGAGTTGACCTCGGTTGTGGAAAATACTTTTCCAACCCAAGATATTGTGCAAACTG TTACAGTCAAACGGAAACGTCGGAAATCACAGTCACATTGTGCTCTGAAAGAACACTCCATGTCTAACAT AGCCAGTGTAAAGAGTCCATATGAGCTGGAGAACGCCGGGGAAGAGCTGGATGCAAGGTTTTCCAAGGCC AAGCCAATGTGCAACACTTGCGGGAAGGTGTTTTCGGAAGCCAGCAGCCTGCGAAGGCACATGAGAATAC ACAAAGGAGTCAAGCCCTACGTCTGCCACTTGTGTGGAAAGGCATTTACCCAGTGCAACCAACTAAAGAC GCATGTGAGGACTCACACAGGGGAAAGGCCATATAAATGTGAACTGTGTGATAAAGGATTTGCTCAGAAA TGCCAGCTGGTCTTCCATAGTCGCATGCATCATGGTGAGGAAAAACCCTATAAATGTGATGTGTGCAATT TACAGTTTGCAACTTCTAGCAACCTCAAGATTCATGCAAGGAAACACAGTGGAGAAAAGCCATATGTTTG TGATAGATGTGGGCAGAGATTTGCCCAGGCCAGCACGCTGACCTATCATGTCCGCAGACACACTGGAGAA AAGCCTTATGTGTGCGACACCTGCGGAAAGGCATTTGCTGTCTCTAGCTCTCTTATCACTCATTCTCGGA AACACACAGGTGAAAAACCATACATATGTGGTATTTGTGGGAAAAGTTTTATTTCCTCAGGAGAGCTCAA CAAACACTTTCGATCCCATACAGGAGAACGACCATTTATCTGTGAATTATGTGGAAACTCATACACAGAC ATTAAAAACTTAAAGAAGCATAAAACAAAGGTCCATTCTGGTACTGATAAAAATCCAGATTGCAGTGTAG ATGACCATGCTGTAAGCGAACAAGACTCCGTACAGAGAAGTCCCTTGTCAGAAACACTAGATGTGAAGCC TTCTGATATGACTCTACCCTTAGCGCTTCCACTTGGGACTGAAGACCACCAGATGCTCCTGCCTGTCACG GACAGTCAGTCTCCTGCATCAGACACGCTGCTGAGGTCCACGGTGAATGGGTACTCGGAACCGCAGTTGA TTTTTTTACAACAGTTGTACTGACTTTGAGGGCATGGACTTGCTAGGCCGTCCTCGGCAGAGACATAACT ATGTCTGGTAAGGTGCAGAGTCGTGTTAACTAACCCCCACTCATTTGAGCCGTGAGCCCATTAGTCTTTG TTCACGGAAGTGAAAGCACCTGACCCTGCATCAGGCAGTGCTGGCTTGGGTTTTTGGCTTTTATTGTAAA CAGCCAGCCTGTGAAGGAGCGCTTCCCCCGCACCGCACCGCACTGTTGTGGGTGGGTCGGTTTTAGACAC TGTTAATGCTGCCTTAATTCCATCTTCACTTTGCATTGTAGACTTGTTTGTTCCTTAGGTCGAATCTGAC CTGGGGTTTTTTGTTGTTCTCTCCTTGCTGTTTAAACAAAATCTACAGTAATATCAACTTCGAAGTGTTT CTGTTTGTAAGCCCTGTGGACCAGTATGGAAACTGTCGGAGGGATTCAAGTTCATGTTTATGTCTATGAA AATTTAGATACTGAGGAAGAATTGTGCTATTTATTAATATATTCCTTTAGAGGTAACTTTTCTAATGAAG CCAAACAAAGTATTTCTCTTAAAACCCAGAAAACGTGTGTAAATTTGTGTTCATTTAGAGTGAATTCAGT ATTCTGGAGAAAACCAGTGCTCAGATCCATTTTTGTTTGAGTTGAAACTATATTTTTTTAAATAGATATT TGGTGTTTTTAAGTGGTCAGAAGACAGTTTTGGAATTTGATTCATTTTATATTTTATTAGATTTGTAGAT TATTGGAAGTGAATATATAACAAGTTTTGAAAATTTTTGATAAGCCTATCTTGTATCAGTTATGCACTTT TTAAAATAAATGTTCTAAAAGGAATTTTCTAGATATAAAATACATACTAGAAATCACTTGGCTTCTAAAT TTGATGTATTACTTAGAAAAATCAAGAAACCTGAGTTGCATGCCTAAGGCAACACACATACACACAAATA AATATCCTTAATATCCTAACTTCAATTATAAGCTATACTTTTGGGTTATCAGTTTGTTTACCATTTTATA TTTGAGTTTTCTATAGACATGTGTTTTTCAAATTTATAAATTAAAACTGCAACGTTTGGTTGTTGGCTAT AGCTTTATAGTGTCCTTTCCGTTCCCCTGGGTGTAGCGAGTTCAGCATCGCCCGTCCTCCAGCCTGCTCC CTCTTCCCCTCTCACTGTCTGCATATCAGTTGATTTCACTTTGCATTTTAAGTTCAGTTTCCCAGTCAGT TCCATTGCTGATAAGTCCTGGTGACTGTTTCTCATCTCTGCCATCACTTAGTTAGGTCTCACGCGTGTTC AGGACTGCACCAGAACAGGCTCGCAGTGTTCACTGGGACTATTCTTCCATCATAATCCTCCCTCTTTGGT TCCAAGTCACAGAACTAAGAAGACATACTAGGACCCTCAGTTCTTGCAAACTGCAGGTGTCTTCCCATTT CTTAAAGCTATAGCATTTTCACGGTAAATTATGACCAGCGAGACTAACTTAACTGGAGAGAGTATGAAAT ACCACCTTTGTACAATAAACTTATTTTATTATTTAGCTAAGAAATCTGTGTGTATGTTAAATGAACATAC TGAGTTTGAATAAAATTTGTAAGGTATAAAATGTTCAGCTAATCTTTTTCTGGTTAAAATCAATAAAAAT TTACTGGAATATAACAAAACTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6848601 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6848601 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007036 same experiment
	EMAGE:21066 same embryo
	EMAGE:21067 same embryo
	EMAGE:21068 same embryo
	EMAGE:21070 same embryo
	EMAGE:21071 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source