EMAGE mouse gene expression spatial database

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EMAGE:21132

Dnajc9 DnaJ (Hsp40) homolog, subfamily C, member 9 ( MGI:1915326)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001729. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001729_01	euxassay_001729_02	euxassay_001729_03	euxassay_001729_04

euxassay_001729_05	euxassay_001729_06	euxassay_001729_07	euxassay_001729_08	euxassay_001729_09

euxassay_001729_10	euxassay_001729_11	euxassay_001729_12	euxassay_001729_13	euxassay_001729_14

euxassay_001729_15	euxassay_001729_16	euxassay_001729_17	euxassay_001729_18	euxassay_001729_19

euxassay_001729_20	euxassay_001729_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21132_wholemount_strong.wlz

21132_wholemount_moderate.wlz

21132_wholemount_weak.wlz

21132_wholemount_possible.wlz

21132_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21132_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

midbrain ventricular layer

moderate

regional

moderate expression: see section 5 6 7 8 9 0 1 2 3 4 5

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4138
Entity Detected:	Dnajc9, DnaJ (Hsp40) homolog, subfamily C, member 9 ( MGI:1915326)
Sequence:	sense strand is shown >T4138 GGCGGGAAAAGGCGGCCGGCGGTCCGCTGTCCAGCAGAGCGGCTGTGGCTACAGCTTCCCCGGGTTCGCG GAGCAGCCTCTTCCCTGGCATGGGGCTGCTGGAGCTGTGCGAGCAGGTGTTTGGCACCGCCGACCTCTAC CAAGTGCTGGGCGTGCGGCGCGAGGCTTCGGACGGCGAGGTCCGACGCGGCTACCACAAAGTGTCCCTGC AAGTGCACCCCGACCGAGTAGAGGAGGACCAGAAAGAGGACGCCACCCGCCGCTTCCAGATCTTGGGAAG AGTCTACGCGGTTCTGAGTGACAAGGAACAGAAAGCAGTGTACGATGAACAGGGGACAGTGGACGAAGAC TCTGCTGGCCTCAACCAAGACCGGGACTGGGATGCATATTGGAGATTACTCTTTAAAAAGATATCGCTAG AGGATATCCAAGCTTTTGAAAAGACATACAAAGGCTCCGAAGAAGAGCTAAACGATATTAAGCAGGCCTA TCTGGACTTCAAGGGCGACATGGACCAGATCATGGAGTCTGTACTTTGTGTACAGTACACAGATGAACCC AGGATAAGAAACATCATTCAAAAAGCTATTGAATCCAAAGAGATTCCAGCCTACAGCGCCTTCGTCAAAG AGTCTAAACAAAAGATGAATGCAAGGAAAAGGAGGGCTCAGGAAGAGGCTAAAGAAGCAGAGTTGAGCAG AAAGGAGCTGGGACTGGAAGAAGGAGTGGATAACTTGAAAGCACTCATCCAGAGCAGACAAAAGGATCGG CAAAAGGAAATGGACAGTTTTCTGGCTCAAATGGAAGCAAAATACTGCAAACCTTCCAAAGGAGGGAAAA GAACAGCACTCAAGAAGGAAAAGAAATGATGGAGTTTTTCTCTTCCTGAGTCCTTATGTGTCAGTGGTAC AGTGTAGACAAAATGATGACAGCCATTAATGTAACCTTTGAGAGGAGTTGTCTGTCCCGCCTAAGCCGAG CAGGGTTCATCGACCTGATCTTAGTAGTTTCTAGAATTCGCCTAACACTGTGTGAGGGTTTCATGTGAAG GATGTGTAGTGGTGATAGTTGGGAGGGTGGGTCTGACATTTGCTTTTGTGGGCCCCTATTTAGAATATTA GTTTCTCTTGCTGCTGTGCATGCACTGACGAGAAACACTCCACCTATGGAGAACATCCTCAGAACACTTG CTGAACCGCTGACTTCTAAATAAATGCTACCGTGCTTTTGGGTCTTAATCCTACACGGATCTTCTTTTCT TGGTCTACTGTTAGGTTTTGTCTTCTGTCCTTGCAGTAGGAGACGTCAGTGTTCATATTATTGCAAACTT TTCATAAGACTGTATATGCTAAATAAAGAAGAAATGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4481661 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481661 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001729 same experiment
	EMAGE:21133 same embryo
	EMAGE:21134 same embryo
	EMAGE:21135 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source