EMAGE mouse gene expression spatial database

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EMAGE:21134

Abhd4 abhydrolase domain containing 4 ( MGI:1915938)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001761. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001761_01	euxassay_001761_02	euxassay_001761_03	euxassay_001761_04

euxassay_001761_05	euxassay_001761_06	euxassay_001761_07	euxassay_001761_08	euxassay_001761_09

euxassay_001761_10	euxassay_001761_11	euxassay_001761_12	euxassay_001761_13	euxassay_001761_14

euxassay_001761_15	euxassay_001761_16	euxassay_001761_17	euxassay_001761_18	euxassay_001761_19

euxassay_001761_20	euxassay_001761_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21134_wholemount_strong.wlz

21134_wholemount_moderate.wlz

21134_wholemount_weak.wlz

21134_wholemount_possible.wlz

21134_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21134_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 0 1 2 weak expression: see section 3

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 0 1 2 weak expression: see section 3

olfactory cortex marginal layer

weak

regional

weak expression: see section 2

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 9 0 1 5 6

telencephalon ventricular layer

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 weak expression: see section 2 4 5 6 7 8

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 6 7 8 9 0 1

pons ventricular layer

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 0 1 2 3 4 5 6

midbrain ventricular layer

moderate

regional

moderate expression: see section 6 7 8 9 0 1 2 weak expression: see section 4

trigeminal v ganglion

moderate

regional

moderate expression: see section 1 2 3 4 5 weak expression: see section 6 7 5 6 7 8 9 0

spinal cord ventricular layer

moderate

regional

moderate expression: see section 6 7 8

dorsal root ganglion

moderate

regional

moderate expression: see section 3 4 weak expression: see section 5 6 7 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4144
Entity Detected:	Abhd4, abhydrolase domain containing 4 ( MGI:1915938)
Sequence:	sense strand is shown >T4144 ATGGCTGATGATCTGGAGCAGCAGCCTCAGGGCTGGCTGAGTAGCTGGCTCCCCACTTGGCGCCCCACTT CCATGTCTCAACTGAAGAATGTGGAAGCCAGGATCCTCCAGTGTCTCCAGAACAAGTTCCTGGCCCGTTA TGTATCCCTCCCAAACCAGAACAAGATCTGGACGGTGACTGTGAGCCCAGAACAAAAGGATCGCACCCCT CTGGTGATGGTACATGGCTTTGGGGGCGGTGTGGGCCTCTGGATCCTCAACATGGATTCACTGAGTGCCC GCCGCACACTTCATACCTTTGATCTGCTTGGTTTTGGGCGAAGCTCAAGGCCAACATTCCCAAGGGACCC AGAAGGAGCTGAAGATGAGTTTGTGGCCTCAATAGAGACATGGCGGGAGACCATGGGAATCCCCACCATG ATCCTCCTGGGGCACAGTTTGGGAGGATTCCTGGCCACTTCTTACTCTATCAAGTACCCTGAAAGAGTTA AACATCTTATCCTGGTGGATCCATGGGGCTTTCCCCTACGACCAACTGACCCTAGTGAGATCCGTGCACC TCCAACCTGGGTCAAGGCTGTGGCATCTGTCCTGGGACGTTCCAATCCACTGGCTGTTCTTCGAGTGGCT GGGCCTTGGGGGCCTGGGCTGGTGCAGAGATTCCGTCCAGACTTCAAGCGCAAGTTTGCAGACTTCTTTG AGGATGACACCATCTCGGAATACATCTACCACTGCAATGCACAGAATCCCAGTGGGGAAACGGCATTCAA AGCCATGATGGAGTCCTTTGGCTGGGCCCGGCGCCCCATGTTGGAGCGAATCCACTTAATTCGAAAAGAT GTGCCCATCACCATGATCTATGGGGCCAACACCTGGATAGATACCAGCACAGGGAAGAAGGTGAAGATGC AAAGGCCGGATTCCTACGTCCGAGACATGGAGATCGAGGGCGCATCCCACCACGTCTATGCTGACCAGCC ACACATCTTCAATGCTGTGGTAGAAGAGATCTGCAACTCAGTTGACTGAGATGCCCTGAGAGGGAGAGGA GGAAGCCAGAGTTGCTGTCATCTGTGTCCCTGTCTTAGTCAGAACCTCTGTCCTTTCCTCACCAACTAAC ATGTGCCAGCCAGGCAGAGTCTCGGGCTGCCCCCAAGATAGGACCACAGTGAACAAGAGCACTTCCAATC CCATGCAGAGGGCAAGAGGCAGAATCCACCAAAGGCCTTGAACTACCCATGCTGGGGAAAAGCATAAAGG GCTGCCTAGTACCGCCCGTTCCCTCCACGCCAGGTGGAGACTACAAGGAGACTGGGCGAACCACACCGTC TCCCTGCATGGCCTTCCCTTCCTCTAGACAGAGGAGGGCTCCCAGCAGCCTTTCTAGTTTGGCAGTGCGA CTGAGAGAAGATTCCACACAGGAACATCTTTCTTCTCCATCACTCCACGCCTCCATCCAAGCTCAGTTTC CACTCGCCCACCAGAAGCAGCTCTAGTCACCTGCACAACCGCTTCCATGACCTTGGAGGTATATCCCAAG CTCTCATTGGCAGATGAGGCAAGAGATAAGGGCAGGATATAGCACATGATGGCTTCCAGCAGAACTGAGT CTATAGCCCAGGTCTGCTGGCTTCCAGCACATGTTGTACACTAAGATTTCATGCAGACACCATTGCTGTG GAGCCGATTGAAATTTAACCCTGCTCCATATTCTAACCTGTTATGCAGGTACACTGTTATCTTGCCCTGT GTCTCAATCCTGGTTGTCTAAGTTGGGACACCTGAGTCTCTTCCTCTTGTTCCTTCCACTATGAGGCTCT GAGCCTCTCTGTGCCTTGGGCCCTAAAGCCCCATGTGTAGTATAGAGCAAAGGTGATTCCTGGTGGAGAA GGGGAAAAGCCCTTCTACCCTAGAGTCATGCCTGGATCCACAACTCCACAGGTATCTGCAGCATTTTCTA CCTGGCCCTATCGCTGAGAGCCCCTCCCCATGGCTCACTGAGGAGCACTCAGCCAGCAGTTCTCTATCCT TATCAGCCACAGAATGCTTCTCACTGCCCGAACCTCTATGCTCTCATCTGTCACCATGGCCTCCAACTCC TTCTGCTTCAAAATTATCCATCAGCTCTCTCCAGTGAATCTCATTCCAGGTCCTGCTCTAAGGCTCTGGG GACTAGGCTGGGCCTGGCCCCAGAAGCCGGGCAGTTCCTGCCTCCTTCTCCACTTTTATAAAGCCAAGCC TTTCCCCAGAATAGTATTAACTCCTGGTGCTTTGTACTACTGGTTCAGCGGCCTCGGGCTTCTTTTCTAT ATTAATAAAGAAGAGTAAAAACTGTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4482138 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4482138 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001761 same experiment
	EMAGE:21132 same embryo
	EMAGE:21133 same embryo
	EMAGE:21135 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source