EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21135

Sgip1 SH3-domain GRB2-like (endophilin) interacting protein 1 ( MGI:1920344)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001827. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001827_01	euxassay_001827_02	euxassay_001827_03	euxassay_001827_04

euxassay_001827_05	euxassay_001827_06	euxassay_001827_07	euxassay_001827_08	euxassay_001827_09

euxassay_001827_10	euxassay_001827_11	euxassay_001827_12	euxassay_001827_13	euxassay_001827_14

euxassay_001827_15	euxassay_001827_16	euxassay_001827_17	euxassay_001827_18	euxassay_001827_19

euxassay_001827_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21135_wholemount_strong.wlz

21135_wholemount_moderate.wlz

21135_wholemount_weak.wlz

21135_wholemount_possible.wlz

21135_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21135_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

homogeneous

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

trigeminal v ganglion

weak

regional

weak expression: see section 2 3 4 5 6 7 7 8 9

spinal cord

weak

homogeneous

weak expression: see section 4 5 6 7 8 9 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4141
Entity Detected:	Sgip1, SH3-domain GRB2-like (endophilin) interacting protein 1 ( MGI:1920344)
Sequence:	sense strand is shown >T4141 GGGAGACTGTAGGTGAAGCAGCACTAGCTGCATCCATGGCCGGTCTTTCGGGTTAACACTTGTCTCCTTT GGCTTGGCAGCAAGCAGAGCAGACCTCAGCGGCAGCTTTGTGAGGACTTAGCTGGGACCTGGAAGCGTAT CCTCCTGTGTTTTTTCTGACTTCTTGGAAAATAAGGAATGCAATTCTGCCACCATGATGGAAGGACTGAA AAAACGTACAAGGAAGGCCTTTGGAATACGGAAGAAAGAAAAAGACACTGACTCTACAGGCTCGCCAGAC CGAGATGGAATGCAGCCCAGCCCACACGAGCCACCCTACCATAGCAAAGCAGAGTGTGCGCGGGAAGGAG GGAAGAAAGCTTCGAAGAAAAGCAATGGGGCACCAAATGGATTTTATGCAGAAATCGATTGGGAAAGATA TAACTCACCTGAGCTAGATGAAGAAGGTTACAGCATCAGACCTGAGGAACCAGGCTCTACCAAAGGAAAG CACTTTTATTCCTCAAGTGAATCTGAAGAGGAAGAAGAGTCGCACAAGAAATTCAACATCAAGATTAAAC CCTTGCAATCTAAGGACGTCCTGAAGAATGCAGCGACGGTAGATGAGCTGAAGGCTTCAATAGGCAACAT CGCACTTTCCCCTTCGCCTGTGAGGAAAAGTCCGAGGCGCAGCCCGGGTGCAATTAAAAGGAACTTATCC AGTGAAGAAGTTGCAAGACCCAGGCGTTCCACCCCAACTCCAGAACTTACAAGCAAGAAGCCTCTGGATG ACACTCTGGCCCTTGCTCCCCTCTTTGGTCCACCATTAGAGTCAGCTTTTGATGAGCAGAAGACGGAAGT TCTTTTAGATCAGCCTGAGATATGGGGTTCAGGCCAACCGATGAACCCAAGTACGGAGTCGCCAGAGCTA GCAAGACCTTTCCCCACTGGAACACCTCCACCTCTGCCTCCAAAAACTGTACCAGCCACTCCGCCTCGAA CAGGCTCCCCCTTAACAGTTGCAACAGGAAATGACCAGGCAGCCACAGAGGCCAAAATTGAGAAACTACC ATCCATCAGTGACCTGGACAGCATTTTTGGGCCAGTGTTGTCGCCCAAGTCTGTTGCTGTTAATACTGAA GAGAAGTGGGTCCATTTTTCTGATGCATCCCCGGAACATGTTACTCCAGAGTTGACTCCAAGGGAACAGG TGGTGACCCCACCAGCTGCATCAGACATCCCAGCTGACTCCCCAGCTCCAGCCCCACCTGGCCCCACAGG CTCCGCAGGTCCCCCAGGGCCTCCTGGTCCTCGCCATGTACCATCTCCGCTCAATTTAGAAGAAGTCCAG AAGAAAGTTGCTGAGCAGACCTTCATTAAAGATGATTACTTAGAAACACTCTCATCTCCTAAAGAGTGTG GGTTGGGACAGAGAGCAACTCCACCTCCCCCACCACCACCCACCTACAGGACTGTGGTCTCGTCCCCCGG ACCTGGCTCGGGCAGTGGTACGGGGACCACCAGTGGTGCGTCTTCTCCTGCTCGACCAGCCACCCCCTTA GTTCCTTGCAGCACCACTCCGCCTCCACCTCCTCCCCGGCCTCCATCCCGGCCAAAGCTACCTCCAGGAA AGCCTGGAGTTGGAGATGTGTCCAGACCTTTTAGCCCACCCATACACTCCTCCAGCCCTCCTCCGATAGC ACCCTTAGCCCGGGCTGAAAGCACTTCTTCAATATCGTCAACTAACTCACTGAGCGCAGCCACCACTCCC ACAGTTGGTTCCTCCCGGGGGCCCAGTCCCCTAACTATGGGGGCCCAGGACACCCTCCCGGTCGCAGCAG CTTTCACAGAAACTGTCAACGCCTACTTCAAAGGAGCAGATCCAAGCAAGTGCATTGTTAAGATCACGGG AGAAATGGTGTTGTCCTTCCCTGCTGGTATCACCAGACACTTTGCCAACAACCCATCCCCAGCAGCTCTG ACTTTCCGGGTGGTAAATTCCAGCAGGTTAGAGCACGTCCTGCCGAACCCCCAACTTCTCTGCTGTGATA ATACACAAAATGATGCCAATACCAAGGAATTCTGGGTAAACATGCCAAATTTGATGACTCACCTAAAGAA AGTCTCTGAACAAAAACCCCAGGCTACATACTATAATGTGGACATGCTCAAATATCAGGTGTCTGCCCAG GGCATTCAGTCCACACCTCTGAACTTGGCGGTGAACTGGCGCTGTGAGCCTGCGAGCACTGACCTGCGCA TAGACTATAAGTACAACACGGATGCCATGTCCACTGCAGTGGCCCTCAACAACGTGCAGTTCCTGGTCCC CATAGACGGAGGAGTGACCAAGCTTCAGGCTGTGCTTCCTCCAGCAGTCTGGAATGCTGAACAACAAAGA ATATTATGGAAGATTCCCGATATCTCCCAGAAGTCAGAAAATGGAGGCGTAGGTTCTTTACTGGCGAGAT TTCAGTTATCCGAAGGCCCGAGCAAACCTTCCCCGCTGGTTGTGCAGTTCACGAGTGAAGGAAGTACTCT CTCTGGCTGTGACATTGAACTTGTTGGAGCAGGCTACCGGTTTTCACTCATTAAGAAGAGGTTTGCTGCA GGAAAGTACTTGGCAGATAACTAATAAAAGGTCATGCAAGGATTTGGAAGATCCATGCCCTGGAGAACTG TTGTTTGAGAGACATGTTTTAATCTGGCTTGATGAACACAAACCGATGTCCGTACTTGGGGTCTGTCAGC TGGAAAGTCCCGGCATTCAGCAGTGACTTCCGGCACCCAGTACTGTGCTGCTCAGTTCTGCAGTATTTAC TTATAGGTGTAATATTTGTTAATGGTTTTAAAATGTAATTATTGTATTTGTAAACTGTACCTTCATTCCA GTAAGGCAGTTAGACACTTGGGTTTTAGCATTTTCCATTCCTGAAACGGATGTAATAATTTAATAGTAGT ACTGTTGAACGGCACAATGCTTACAGAGATACAGCGCATTTTGTCAATATATAAAATTTAAATATAATGT TGATAGTTATCATAAAGGGGGTGCCACACATCGAGAACCTTAAACAGAACCAGAAACAAAACACCCAGCT AACTTTCCTTCACTCTTTACTATGTTTCCCTCTAGTGCTAAAGAAAACTTCTAGCTTCAGTTTAGTGGGT TAAATTCACAAACTATTTCAGAGGAAAAAAAATTCTAAAGTTACAGCCTATTCAAAGAGAAACATTAATT ACCACTTTTTAAAGAGCTTTTTTTTCAAACTGCAAATTTCATAAAAATGCAAACTGTGTAAACAGGGCCT CTTATTTTTATAACTTGTGTAAAAAGGGAAAAGCAATTCATATTTAAAGTTTAAGTATATTAAATTATAT CCAAGAGGGCAGAGGATGCTGAAGTCTGACCTGACCCCAGCCCCTTCTGTGCCGTTTAGCAAATGTGGAG ATTGCTAAGTCATCGCATTAAAGCCAACATGATTTTTAAAGTTTGAGACTTTATGTAGCTGAACTGGTTA AAACTTTTGCACAATTGCTCGGGACACAGGGGCCTCTCTCGTCCAGCATCCAGGGGTGGGTACCTTGTTT CTTCTTTACTCATGAGAATCAAAACACAGAGAAGGATGGAGGAAAACAAATAGTCCCCTGGTCCATCCAA AAGGAGAATTCTAAACTTATCATTTACATCTTTGGGGGGAAGGAAGAAATAAACTTTATAAATGAAATTC TAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4482298 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4482298 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001827 same experiment
	EMAGE:21132 same embryo
	EMAGE:21133 same embryo
	EMAGE:21134 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source