EMAGE mouse gene expression spatial database

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EMAGE:21259

Msto1 misato homolog 1 (Drosophila) ( MGI:2385175)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004884. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004884_01	euxassay_004884_02	euxassay_004884_03	euxassay_004884_04

euxassay_004884_05	euxassay_004884_06	euxassay_004884_07	euxassay_004884_08	euxassay_004884_09

euxassay_004884_10	euxassay_004884_11	euxassay_004884_12	euxassay_004884_13	euxassay_004884_14

euxassay_004884_15	euxassay_004884_16	euxassay_004884_17	euxassay_004884_18	euxassay_004884_19

euxassay_004884_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21259_wholemount_strong.wlz

21259_wholemount_moderate.wlz

21259_wholemount_weak.wlz

21259_wholemount_possible.wlz

21259_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21259_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

weak

regional

weak expression: see section 1 2 3 4 5 2 3 4 5 6 7 8 9 0

vertebral axis musculature

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6881
Entity Detected:	Msto1, misato homolog 1 (Drosophila) ( MGI:2385175)
Sequence:	sense strand is shown >T6881 GGAGTGCCCAAGAGCAGCGGCGGTATGGCGGGCGGGGCCCGCGAGGTGCTCACGCTGCAGTTGGGACATT TCGCGGGTTTCGTGGGGGCGCACTGGTGGAACCAGCAGGATGCTGCGCTGGGACGCATGGCCGAGGACGA AGAGTCGCCAGGCGAGCTGTGCCCCGACGTCTTGTACAGAACAGGCCGGACACTGCATGGCCAGGAAACC TATACGCCTCGACTCATCCTAATGGATCTGAAAGGTAGTCTGAACACTCTAAAAGAAGAAGGTAACCTCT ACAGAGACCGACAGCTAGAGGCTGCAGTAGCGTGGCAGGGGAAGCTCAGCACACACAGAGAAGACGCCCA ACCAAAGAACCCAAACCTCCAGGGCCTTCTGAGTGCAGAGGGAGTGCGGAGTAGTGACGGTGCCTGGAGG GCCAAACTGATTCAGAATATCCAAAATGGCAAAGAGAACAGCATCAAAGTCTGGTCAGACTTCCTCAGAG TCCATCTCCATCCACGGAGCATCTGTGTGATCCACAAGTACCACCATGATGGGGAGACAGGCCGGCTGGA GGCTTTTGGCCAAGGGGAGAGTGTCCTGAAGGAGCCCAGGTACTTGGAAGAGCTGGAGGACAGACTACAC TTCTATGTGGAGGAATGTGACTACTTGCAGGGCTTCCAGCTCCTGTGTGACCTGCACGATGGCTTCTCTG GAGTAGGTGCTAAAACTGCAGAGCTACTACAAGACGAGTATGCAGGGCGGGGAGTCCTAACCTGGGGCCT GCTCCCGGGCCCCTACAGTCTTGGGGAGCCTCAGAAAAACATCTATCGTCTCTTAAACACGGCATTTGGT CTGGTGCACCTGACTGGATACAGCTCCTTCGTCTGTCCCTTATCCCTTGGAGGGAACCTGGGCCTGCGAC CCAAGCCGCCTGTCAACTTCCCTTCCCTACATTATGATGCCACTCTGCCCTTCCACTGTAGCGCCATCCT GGCGACAGCGCTGGACACGGTGACTGTTCCCTATCGCCTGCGCTCCTCAATGGTTACCATGGCTCACTTG GCTGATGTGCTAAGTTTCTCTGGGAAAAAGGTGGTGACAGCAGAAGCAATCATCCCCTTCCCACTGGTTC GAGGCCAGTCCCTTCCTGACATCCTGACGCAGCTTGGAGAAGCCACCCCATGGACCTCCCTGTCGGCATG CGGGGATTCTGCTGGACATCGGTGCTTTGCCCAGTCTGTGGTGCTCAGAGGCATAGACAGAGCTAGCCAC ACCAGTAAGCTCAACCCAGGGACACCTCTGCCCTCTGCTCTCCACGCATGCGCCTCTGGAGAAGAAGTCT TGGCCCAGTACTTACAGCAGCAGCATCCTAGAGTCTTGAGTTCGTCACATCTGCTGCTGACTCCCTGCAA AGTGGCTCCTCCATACCCACACTTCTTCTCAAGCTTCAGTCAGAAAGGGCTGGCCATGGACAGTACCCCC AAGGGCGCAGCAGTACAGAGCATCCCTGTGTTTGGGGCCTTGCGTTCAACTTCATCCTTGCACCGTACGC TGGGAGACTTGGCTGAAGAGCTCAGCAGACTTGACCTGCGGCGCTGGGCTAGCTTCATGGACGCTGGCGT GGAGCAGGATGACATGGAAGAGATGCTGCACGAGTTACACAGGTTGGCCCAGTGTTACCAGGAGGGTGAC AGTCTCTCGAACTGAAGTCCAGGGGAGGGTAGTGGGAGAGAATTTATAATAAAAGCTGCCGTGCAGGAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3966815 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3966815 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004884 same experiment
	EMAGE:21257 same embryo
	EMAGE:21258 same embryo
	EMAGE:21260 same embryo
	EMAGE:21261 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source