EMAGE mouse gene expression spatial database

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EMAGE:21260

Prep prolyl endopeptidase ( MGI:1270863)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004891. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004891_01	euxassay_004891_02	euxassay_004891_03	euxassay_004891_04

euxassay_004891_05	euxassay_004891_06	euxassay_004891_07	euxassay_004891_08	euxassay_004891_09

euxassay_004891_10	euxassay_004891_11	euxassay_004891_12	euxassay_004891_13	euxassay_004891_14

euxassay_004891_15	euxassay_004891_16	euxassay_004891_17	euxassay_004891_18	euxassay_004891_19

euxassay_004891_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21260_wholemount_strong.wlz

21260_wholemount_moderate.wlz

21260_wholemount_weak.wlz

21260_wholemount_possible.wlz

21260_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21260_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 6 7 8 9

vibrissa

moderate

regional

moderate expression: see section 4 5 6 7 8 9 weak expression: see section 3

facial vii ganglion

strong

regional

strong expression: see section 3 4 7

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 4 5 2 3

trigeminal v ganglion

strong

regional

strong expression: see section 2 3 4 5 6 3 4 5 6 7 8

vagus x ganglion

strong

regional

strong expression: see section 5 moderate expression: see section 1

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 4 5 2 3 4

dorsal root ganglion

strong

regional

strong expression: see section 3 4 5 6 7 8 9 0 1

heart ventricle

moderate

regional

moderate expression: see section 5 6 7 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6884
Entity Detected:	Prep, prolyl endopeptidase ( MGI:1270863)
Sequence:	sense strand is shown >T6884 GTGAGTCCGCCCGTGCACGCCGGCTGTCCGCTCGCCCGCTCGCACGCCGCCCTCCGCCGCAGTCCGCGCC CAGCCTCGCCCGCACTTCCCCCCTTCAGCCCGCGCCCGGATCCGGCCATGCTGTCCTTCCAGTACCCCGA CGTGTACCGCGACGAGACCTCCGTGCAGGAGTATCATGGACATAAAATTTGTGACCCGTATTCTTGGCTT GAAGACCCAGACAGCGAGCAGACTAAGGCTTTCGTGGAGGCACAAAACAAGATTACTGTGCCATTTCTTG AGCAATGTCCAATCAGAGGTTTATACAAGGAGAGAATGACTGAGCTGTACGACTACCCCAAGTACAGCTG CCACTTCAAGAAAGGAAAGCGGTACTTTTATTTTTACAATACAGGTTTGCAGAACCAACGCGTGTTATAT GTGCAAGACTCCTTAGAGGGGGAGGCCAGAGTGTTCCTGGACCCCAACACACTGTCGGATGATGGTACAG TGGCACTCCGAGGTTATGCCTTCAGTGAAGATGGCGAATACTTTGCCTATGGTCTGAGCGCCAGCGGCTC CGACTGGGTGACGATCAAGTTCATGAAGGTCGATGGTGCCAAAGAGCTCCCGGATGTGCTGGAGAGAGTC AAGTTCACCTGCATGGCCTGGACGCATGACGGGAAGGGAATGTTCTATAACTCATACCCGCAGCAGGACG GGAAGAGCGATGGCACAGAGACATCCACCAATCTCCACCAAAAGCTCTGCTACCACGTCTTGGGGACGGA TCAGTCAGAAGATATTTTGTGTGCTGAGTTCCCCGATGAGCCTAAATGGATGGGAGGAGCTGAGCTATCC GACGATGGCCGGTACGTCTTGTTATCCATCTGGGAGGGATGTGATCCAGTAAACCGACTGTGGTACTGTG ACCTCCAGCAAGAACCCAATGGTATCACCGGAATCCTGAAGTGGGTAAAACTGATAGACAACTTTGAAGG AGAATACGACTACGTAACCAACGAGGGCACAGTGTTCACATTCAAGACGAACCGCAATTCTCCCAATTAT CGGCTGATCAATATCGACTTCACGGACCCTGACGAGTCCAAGTGGAAAGTGCTCGTACCGGAGCACGAGA AGGATGTCTTAGAATGGGTGGCTTGTGTCAGGTCCAACTTCCTGGTCTTGTGCTACCTCCATGACGTGAA GAACATTCTACAGCTTCACGACCTGACGACTGGTGCTCTCCTCAAGACCTTCCCCCTGGATGTGGGCAGC GTGGTGGGCTACAGTGGGCGCAAGAAGGACTCTGAGATCTTCTACCAGTTCACATCCTTCCTATCTCCAG GTGTCATTTACCACTGCGATCTTACCAAAGAGGAACTGGAGCCCATGGTTTTCCGAGAGGTGACGGTGAA GGGAATCGATGCTGCTGATTACCAGACAATCCAGATTTTCTACCCTAGCAAGGATGGCACCAAGATTCCC ATGTTTATTGTGCATAAAAAAGGCATAAAGCTGGATGGCTCACACCCTGCTTTCTTATATGGCTACGGGG GCTTCAACATCTCCATTACACCCAACTACAGCGTGTCCAGGCTCATTTTTGTGAGACATATGGGCGGCGT CCTCGCGGTGGCTAACATCAGAGGCGGCGGTGAATACGGAGAGACGTGGCACAAAGGTGGTATCTTGGCC AACAAACAGAACTGTTTTGATGACTTCCAGTGTGCTGCTGAGTACCTCATCAAGGAAGGTTACACATCTC CCAAGAGGCTCACGATCAACGGAGGCTCCAACGGCGGCCTCTTAGTAGCTGCTTGCGCAAATCAGCGTCC AGACCTCTTCGGCTGTGTTATTGCCCAGGTTGGAGTGATGGACATGTTGAAGTTCCACAAATTTACTATC GGCCATGCATGGACCACTGATTACGGGTGCTCCGACACTAAACAACACTTTGAATGGCTTCTCAAATACT CGCCACTACACAATGTGAAGCTTCCGGAGGCAGACGACATCCAGTACCCGTCCATGCTTCTCCTCACGGC TGACCACGACGACCGCGTGGTCCCGCTGCACTCACTGAAGTTCATTGCCACCCTTCAGTACATCGTGGGC CGCAGCCGGAAGCAGAGCAACCCCCTGCTCATCCACGTGGACACCAAGGCCGGCCACGGGGCCGGGAAAC CCACCGCCAAAGTGATAGAAGAAGTCTCGGATATGTTTGCGTTCATAGCACGGTGCCTGAACATCGAGTG GATCCAGTAAATGGGATTTCCAGTTCCTCCCACCAAACACAGAAAACCTCAAGGGCTTTCACACCATCCT GACCAAGAAACCACTGGGCAGAGCGCTGCCCTACGTGAGCACTGTGCCTCCGCTCACAGACAGCAAGCCG CTGAGCAGAACTGCCACGGGGATTTTATCTTTTCTAGGCTTCATCTTTCTAGCAAGCCCTTGATGTTTTG GGTTGGTTTTTTTTTTCCCACTCTCTCCAGGCACATATCTTTAAAAGGGGGAAAGGGGGGCATGTTTGTA TAAGAAACTAAAAGGCAGTGAACATGTTGTGAATATTAGACTCCTGAATTCGTAAGGGATCTAATAAGGT CATAGTTGGGCATACCCATATATAATAGCTCAATGAAGTTACTTCTCTAACCATAGCATATAAACTTAAA TAAATGGGAGACCTCAAGGGAGTCTCCACTGCAATACTAATAAATGCTATTTAAGAGTGAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3967219 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3967219 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004891 same experiment
	EMAGE:21257 same embryo
	EMAGE:21258 same embryo
	EMAGE:21259 same embryo
	EMAGE:21261 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source