EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21261

Pvrl4 poliovirus receptor-related 4 ( MGI:1918990)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004893. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004893_01	euxassay_004893_02	euxassay_004893_03	euxassay_004893_04

euxassay_004893_05	euxassay_004893_06	euxassay_004893_07	euxassay_004893_08	euxassay_004893_09

euxassay_004893_10	euxassay_004893_11	euxassay_004893_12	euxassay_004893_13	euxassay_004893_14

euxassay_004893_15	euxassay_004893_16	euxassay_004893_17	euxassay_004893_18	euxassay_004893_19

euxassay_004893_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21261_wholemount_strong.wlz

21261_wholemount_moderate.wlz

21261_wholemount_weak.wlz

21261_wholemount_possible.wlz

21261_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21261_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

sublingual gland primordium

moderate

regional

moderate expression: see section 5 6 1 2

adenohypophysis

moderate

regional

moderate expression: see section 7 8 9 0 1

epidermis

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

vibrissa

strong

regional

strong expression: see section 3 4 5 7 8 9

pharyngo-tympanic tube

strong

regional

strong expression: see section 1 6 7 8 9 0 moderate expression: see section 2

naris

strong

regional

strong expression: see section 8 1 2 moderate expression: see section 0 3

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 6 7 8 9 2 3 4 5 6

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 9 moderate expression: see section 8 2 3

stomach

strong

regional

strong expression: see section 1 2 3 4 5 moderate expression: see section 6

rectum

moderate

regional

moderate expression: see section 9 0

midgut

moderate

regional

moderate expression: see section 8

lower jaw incisor

strong

regional

strong expression: see section 7 8 0 1

lower jaw molar

strong

regional

strong expression: see section 5

oral epithelium

strong

regional

strong expression: see section 4 5 6 7 8 9 0 1 2 3 4 5 6 7

upper jaw incisor

strong

regional

strong expression: see section 7 8 1

upper jaw molar

strong

regional

strong expression: see section 5

bladder

strong

regional

strong expression: see section 8 9 0

urethra of male

strong

regional

strong expression: see section 9 0 1

trachea

weak

regional

weak expression: see section 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6885
Entity Detected:	Pvrl4, poliovirus receptor-related 4 ( MGI:1918990)
Sequence:	sense strand is shown >T6885 GTGGGAAGCAGCTCTGGGGGAGCTCAGGGCTCCCGAGCACGGCTTCTTGGGGGTAGCCACGGCTGGGTGG GTAGAACTGGGGCCTGGGTCCCCTAGTGGAGACCCCAAGTGGCATAAGCAGGTACGGGCCGTGCCTGGGG ACTCGCTGACTTCCTGACTTCTGGGTCAGAGCCTTACCCAGCTCCAGAACCGGCTCCCAGGGAGAACTCC GTGGAACTCCAGAGTCACTGGGCAGGTCTACCTTTCAGCCAATGCCCCTGTCCCTGGGAGCCGAGATGTG GGGCCCTGAAGCCTGGCTGCGGCTGTTGTTCCTGGCATCGTTTACAGGCCAATATTCCGCGGGTGAGCTG GAGACCTCTGACGTAGTGACAGTGGTGCTGGGTCAGGATGCAAAACTGCCCTGCTTCTACCGAGGGGATC CGGATGAGCAGGTGGGGCAGGTGGCGTGGGCTCGAGTGGACCCAAACGAGGGTATCCGGGAGCTGGCCCT ACTGCACTCCAAATACGGGCTTCATGTGAACCCTGCTTATGAGGACCGCGTGGAGCAACCACCGCCCCCA CGAGACCCTTTAGACGGCTCGGTCCTCCTGCGCAACGCTGTGCAAGCTGATGAGGGCGAGTACGAATGCA GAGTCAGCACCTTCCCAGCCGGCAGCTTTCAGGCACGGATGCGTCTTCGTGTTCTGGTGCCTCCTCTGCC TTCACTGAATCCTGGTCCACCACTAGAAGAGGGCCAGGGCCTGACGTTGGCAGCCTCTTGCACTGCTGAG GGCAGCCCAGCTCCCAGCGTGACCTGGGACACAGAGGTCAAGGGCACACAGTCGAGCCGCTCTTTCACAC ACCCCCGCTCAGCTGCCGTCACTTCAGAGTTCCATCTGGTGCCCAGCCGAAGTATGAATGGGCAGCCGCT GACCTGTGTGGTGTCTCACCCTGGCCTGCTCCAGGACCGAAGAATCACCCACACCCTCCAAGTGGCCTTC CTTGCGGAGGCCTCTGTGAGGGGCCTTGAGGACCAAAACTTGTGGCAAGTCGGCCGAGAAGGAGCCACAC TCAAATGCCTGAGTGAAGGACAGCCCCCTCCCAAATACAACTGGACACGGTTGGATGGACCTCTGCCTAG TGGGGTACGAGTGAAAGGAGACACTCTGGGCTTTCCCCCACTGACTACTGAGCACAGTGGCGTCTATGTC TGTCACGTCAGCAATGAGCTTTCTTCGAGGGATTCTCAGGTCACTGTGGAAGTTCTTGACCCCGAAGATC CAGGAAAGCAGGTGGACCTGGTGTCCGCCTCAGTGATCATAGTGGGCGTGATCGCTGCTCTCCTGTTCTG CCTCCTGGTGGTGGTGGTGGTGCTCATGTCCCGATATCATCGCCGCAAAGCCCAGCAGATGACCCAGAAA TATGAGGAGGAGCTGACTTTGACCAGGGAGAACTCCATCAGGAGGCTGCACTCTCACCACTCGGACCCTA GGAGCCAGCCGGAGGAGAGTGTAGGGCTGAGAGCGGAGGGCCACCCTGATAGTCTCAAGGACAACAGTAG CTGCTCTGTGATGAGTGAAGAGCCAGAAGGCCGCAGCTACTCCACGTTAACCACAGTGAGAGAGATCGAG ACACAGACCGAACTGCTATCTCCAGGCTCTGGGCGGACAGAGGAGGACGACGATCAGGATGAAGGCATCA AACAGGCCATGAACCATTTTGTGCAGGAAAATGGGACCCTGAGGGCCAAACCTACAGGCAATGGTATCTA CATCAATGGGCGAGGGCACCTGGTCTGACCCAGGCCTGCCTCCCTCCCCGAGGCCTGGCTTCTTCTGCTT CCATGGGGGAACTTCGGCTCTTCTTAGGGGGAAACCCCCTTAAATGCCTGCATTTCTTTAGGAAGATACT TCCCACCCTACTGACTGCTCTACTTTGACCTCCAATCCTTCTGTTCATCAGGAGAGCTCTGCTGGTTGAG TGCCTCCCACCATTGCGTGTAGGTCATCCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT CCGTGCTGTCACGTTAAGAGTCCAAGTGAATGATCTGTGTGCTACGGGATTTGTGGCTGTGTGCAGAGCA TTCCTAGGGTGTGGTGTGTGAGTAGAGTGCCTTATGTGTGACTTCTGCCTGCAGAGGCAGGGATTTTCCT CAGACCCCAGAGCAGTATTAGCGATGCAGAGGTTAGAAGCGAGAGGCGGAGACTGTGGCCAGATCCAGGT GTGCGGGCACAGCTGGAGCTAGAATCTGACCTCCAAAGTGGGGGAGCTGTGTTCCCACCCCTTGGGAGCA AGTGAGAAGCAGACACCGCGGGGGTCTGCCAGAGGCCAGAACTGTTACAGAAGCCCTCTGCCCTCTGGTG GCCTGTGGGCCTGCTGCATGTACGTACCTTCTGTAAATTAAACTCCAAGGGAGCTAGCTTCCTGAGTTCT GCTTAGACTCCCTTAATAAGAGAAAAAGAAACTTTAAAAGTAGAGAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3966993 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3966993 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004893 same experiment
	EMAGE:21257 same embryo
	EMAGE:21258 same embryo
	EMAGE:21259 same embryo
	EMAGE:21260 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source