EMAGE mouse gene expression spatial database

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EMAGE:21298

Gjc1 gap junction protein, gamma 1 ( MGI:95718)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012257_01	euxassay_012257_02	euxassay_012257_03	euxassay_012257_04	euxassay_012257_05

euxassay_012257_06	euxassay_012257_07	euxassay_012257_08	euxassay_012257_09	euxassay_012257_10

euxassay_012257_11	euxassay_012257_12	euxassay_012257_13	euxassay_012257_14	euxassay_012257_15

euxassay_012257_16	euxassay_012257_17	euxassay_012257_18	euxassay_012257_19	euxassay_012257_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21298_wholemount_strong.wlz

21298_wholemount_moderate.wlz

21298_wholemount_weak.wlz

21298_wholemount_possible.wlz

21298_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21298_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

spotted

strong expression: see section 7 8 9 0 1 4 5

telencephalon mantle layer

strong

spotted

strong expression: see section 1 2 3 4 7 8 9 0 4 5 6 7 8 9

olfactory cortex ventricular layer

weak

regional

weak expression: see section 9 4 5

telencephalon ventricular layer

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 2 3 4 5 6 7 8 9 0

medulla oblongata basal plate mantle layer

strong

spotted

strong expression: see section 4 7 8 9 0 1 4 5 6

rest of cerebellum mantle layer

strong

spotted

strong expression: see section 1 2 3 4 7 8 9 0 1 4 5 6 7 8

rest of cerebellum ventricular layer

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8

pons mantle layer

strong

spotted

strong expression: see section 4 7 8 9 0 1 4 5 6

midbrain mantle layer

strong

spotted

strong expression: see section 3 4 5 6 7 8 9 0 1 4 5 6

midbrain ventricular layer

weak

regional

weak expression: see section 4 5 6 7 8 9 0 1 2 3 4

spinal cord mantle layer

strong

spotted

strong expression: see section 7 8 9 0 1

lower jaw incisor

weak

regional

weak expression: see section 9 0

upper jaw incisor

weak

regional

weak expression: see section 9 0 4

renal cortex

weak

regional

weak expression: see section 7 8 9 5 6 7 8

trachea

weak

regional

weak expression: see section 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30389
Entity Detected:	Gjc1, gap junction protein, gamma 1 ( MGI:95718)
Sequence:	sense strand is shown >T30389 GCAAACCCTATGCCATGCGTTGGAAACAGCACCGGGCTCTGGAAGAAACGGAAGAGGACCATGAAGAGGA TCCTATGATGTATCCAGAGATGGAGTTAGAAAGCGAAAAAGAAAATAAAGAGCAGAGCCAACCAAAACCT AAGCATGATGGCCGACGACGAATTCGAGAGGATGGGCTCATGAAAATCTATGTGTTGCAGCTGCTGGCCA GGACTGTGTTTGAGGTGGGCTTTCTAATAGGGCAGTATTTCCTGTATGGCTTCCAAGTCCACCCATTTTA TGTGTGCAGCAGACTTCCTTGCCCTCATAAGATAGACTGCTTTATTTCTAGACCCACTGAAAAGACCATC TTCCTTCTGATAATGTATGGTGTCACAGGCCTCTGCCTATTGCTTAACATTTGGGAGATGCTTCACTTAG GGTTTGGGACAATTCGAGACTCACTAAACAGTAAAAGGAGGGAACTTGATGATCCGGGTGCTTATAATTA TCCTTTCACTTGGAACACACCCTCTGCTCCCCCTGGCTATAACATTGCTGTCAAACCAGATCAGATCCAG TACACTGAGCTGTCCAATGCTAAGATTGCCTACAAGCAAAACAAAGCCAATATTGCCCAGGAACAGCAGT ACGGCAGCCACGAGGAACACCTCCCGGCTGATCTGGAGACTCTGCAGCGGGAGATCAGAATGGCTCAGGA ACGCTTGGACCTAGCAATCCAGGCCTACCATCACCAAAACAACCCCCATGGTCCTCGGGAAAAGAAGGCC AAAGTGGGGTCCAAATCTGGGTCCAACAAAAGCAGTATTAGTAGCAAATCAGGGGATGGGAAGACCTCCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6330453), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58560. Forward Primer - name:058560_F_IRAV102_h01_Gja7, sequence:GCAAACCCTATGCCATGC; Reverse Primer - name:058560_R_SP6_IRAV102_h01_Gja7, sequence:ACGGAGGTCTTCCCATCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012257 same experiment
	EMAGE:21297 same embryo
	EMAGE:21299 same embryo
	EMAGE:21300 same embryo
	EMAGE:21301 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source