EMAGE mouse gene expression spatial database

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EMAGE:21300

Agps alkylglycerone phosphate synthase ( MGI:2443065)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012289. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012289_01	euxassay_012289_02	euxassay_012289_03	euxassay_012289_04

euxassay_012289_05	euxassay_012289_06	euxassay_012289_07	euxassay_012289_08	euxassay_012289_09

euxassay_012289_10	euxassay_012289_11	euxassay_012289_12	euxassay_012289_13	euxassay_012289_14

euxassay_012289_15	euxassay_012289_16	euxassay_012289_17	euxassay_012289_18	euxassay_012289_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21300_wholemount_strong.wlz

21300_wholemount_moderate.wlz

21300_wholemount_weak.wlz

21300_wholemount_possible.wlz

21300_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21300_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 8 9 weak expression: see section 0 1

sublingual gland primordium

moderate

regional

moderate expression: see section 6 7 2 3

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 6 1 2

facial vii ganglion

weak

regional

weak expression: see section 1 6 7

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 4 5 3 4

trigeminal v ganglion

weak

regional

weak expression: see section 1 2 3 4 5 6 3 4 5 6 7 8

spinal cord meninges

moderate

regional

moderate expression: see section 7 8 9 2 3 weak expression: see section 6 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30417
Entity Detected:	Agps, alkylglycerone phosphate synthase ( MGI:2443065)
Sequence:	sense strand is shown >T30417 GGTCTGGCTGCTGGAGAAGATAACGGTCAGAGGGGTTATTTGCTGACTTATGTCATTGCATATATTCGTG ATTTGGGTTTGGAATACTATGTAATAGGAGAATCTTTTGAGACTTCTGCTCCTTGGGACAGGGTTATAGA TCTGTGTAGAAATGTAAAGGAGAGGATAAGAAGGGAATGCAAAGAAAGGGGAGTTCAGTTCGCACCTCTT TCTACGTGCAGGGTGACACAGACTTATGATGCAGGTGCATGTATCTACTTCTATTTTGCCTTTAACTACA GGGGAATTAGTGACCCACTGACCGTGTTTGAACACACTGAGGCAGCTGCTAGAGAGGAAATCCTTGCCAA TGGAGGGAGCCTGTCACATCACCATGGAGTGGGCAAGATACGAAAGCAGTGGCTAAAGGAGAGCATCTCT GATGTCGGCTTTGGAATGCTAAAGTCGGTCAAGGAGTATGTGGACCCAAGTAACATCTTTGGAAACAGAA ACCTTTTATAACTCCATTAATATCATTATGAAAAATGTTACCTTTTAAAAGTCATCAGCTATGGTTATAC CAGTAATTGAATATATCATGGGACTGTATTTCAGCTACATTTGTTTGTTGGTTTAAAATGAGTTGGTTTT TGTTCTGTGCTTTGTTTCTACATCTATAGATTGACAGATGGCGCTCCTGGGCCTCATTGTAGGTGTACCA GTTTATAGCCAACTAACTGGTTGTCGTTTCAGATTTTAGTCAGGCAGCACAAGGCTGCCCATTATCTGAG GAATATGCTGCCAGCTGTTTTACATGCTCCTCCTCTCGGTGAGCTGAGGCAGAGGGTGTCATCCGTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6332574), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60417. Forward Primer - name:060417_F_IRAV104_d05_Agps, sequence:GGTCTGGCTGCTGGAGAA; Reverse Primer - name:060417_R_SP6_IRAV104_d05_Agps, sequence:GCAACGGATGACACCCTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012289 same experiment
	EMAGE:21297 same embryo
	EMAGE:21298 same embryo
	EMAGE:21299 same embryo
	EMAGE:21301 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source