EMAGE mouse gene expression spatial database

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EMAGE:21386

Slc4a1 solute carrier family 4 (anion exchanger), member 1 ( MGI:109393)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_018966_06	euxassay_018966_07	euxassay_018966_08	euxassay_018966_09	euxassay_018966_10

euxassay_018966_11	euxassay_018966_12	euxassay_018966_13	euxassay_018966_14	euxassay_018966_15

euxassay_018966_16	euxassay_018966_17	euxassay_018966_18	euxassay_018966_19	euxassay_018966_20

euxassay_018966_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21386_wholemount_strong.wlz

21386_wholemount_moderate.wlz

21386_wholemount_weak.wlz

21386_wholemount_possible.wlz

21386_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21386_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1

liver

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T51039
Entity Detected:	Slc4a1, solute carrier family 4 (anion exchanger), member 1 ( MGI:109393)
Sequence:	sense strand is shown >T51039 GGGCACCAGTGGCGGCAGGCAGGACTTCCCGAAGGACCCCCGAGGCTCCTGAGCAGGGACCTGAGGTGCA GGTTATGCTGGCGGCCCAGAGAGCAGAGAACGACTGGACACTGAGGACGGCATCATGGGGGACATGCGGG ACCACGAGGAAGTGCTGGAGATCCCAGATCGAGACAGCGAAGAAGAACTGGAGAACATAATAGGACAGAT AGCATATAGAGACCTAACCATCCCTGTGACCGAGATGCAGGACCCAGAAGCTCTTCCCACAGAGCAAACA GCCACAGACTACGTCCCCAGCAGTACCTCCACACCGCACCCAAGCTCCGGTCAGGTCTATGTGGAGCTTC AGGAACTGATGATGGACCAGAGGAACCAGGAACTACAATGGGTGGAGGCAGCGCACTGGATAGGGCTGGA GGAAAACCTTCGAGAGGATGGTGTATGGGGTCGCCCACATCTATCTTACCTGACCTTCTGGAGCCTTCTA GAACTGCAGAAGGTCTTCTCAAAAGGCACCTTCCTTCTGGGTCTGGCAGAGACATCCCTGGCTGGGGTGG CCAATCACCTTCTAGACTGCTTCATCTACGAGGATCAGATTCGGCCTCAGGACCGAGAAGAACTGCTCCG GGCTCTGCTACTCAAACGCAGCCATGCTGAGGACCTAGGGAATCTGGAGGGGGTGAAGCCCGCCGTCCTG ACCCGCTCTGGGGGCGCCTCTGAGCCTCTGCTTCCTCACCAGCCATCGCTGGAGACCCAGCTGTACTGCG GACAGGCAGAGGGGGGCTCAGAAGGACCCTCAACATCTGGGACTCTGAAGATCCCCCCAGATTCAGAAAC CACACTGGTGCTAGTGGGCCGGGCTAATTTTCTGGAGAAGCCTGTACTGGGCTTCGTGAGACTGAAGGAG GCCGTGCCTCTGGAGGACCTGGTGTTGCCAGAGCCTGTGGGCTTCCTTCTTGTTCTGCTGGGACCTGAGG CTCCGCATGTCGACTATACCCAGCTGGGCAGGGCTGCAGCAACTCTCATGACAGAAAGAGTGTTCCGCAT TACCGCCTCCATGGCGCATAACCGAGAGGAGCTGCTGCGCTCTCTGGAGAGCTTTCTGGACTGTAGCCTG GTGCTGCCGCCCACAGACGCCCCTTCAGAAAAAGCTCTGCTTAACCTGGTACCGGTGCAGAAGGAGCTGC TTAGGAGGCGGTACCTGCCCAGCCCCGCCAAGCCAGACCCCAACTTGTACAACACTCTAGACTTGAACGG GGGAAAAGGGGGCCCTGGTGACGAAGACGACCCTCTACGGCGCACAGGCCGGATCTTTGGAGGCCTGATC CGTGATATCCGGCGCCGTTATCCTTACTACCTGAGTGACATCACCGATGCGCTCAGTCCCCAGGTGTTGG CTGCTGTCATCTTCATCTACTTTGCTGCCTTGTCACCTGCCGTCACCTTCGGCGGCCTCCTGGGAGAAAA GACCCGGAACTTGATGGGGGTGTCGGAGCTGCTCATCTCCACAGCAGTGCAAGGCATTCTCTTTGCTCTC CTGGGGGCACAGCCCCTGCTTGTGCTAGGCTTCTCAGGACCCCTGCTGGTGTTTGAAGAAGCCTTCTTCT CGTTCTGTGAAAGCAATAACCTGGAGTATATCGTGGGCCGCGCGTGGATCGGCTTCTGGCTCATCCTGCT CGTGATGTTGGTGGTGGCCTTTGAAGGCAGCTTCCTCGTCCAATACATCTCCCGATACACCCAGGAGATC TTCTCCTTCCTCATCTCCCTCATCTTCATCTATGAGACTTTCTCCAAGCTGATCAAGATTTTCCAGGACT ACCCACTACAACAGACTTATGCCCCTGTTGTGATGAAGCCCAAACCTCAGGGCCCTGTGCCCAACACAGC CCTCTTCTCTCTTGTGCTCATGGCTGGCACCTTCCTACTTGCCATGACGCTACGAAAGTTCAAGAACAGC ACCTACTTCCCTGGCAAGCTGCGTAGAGTCATTGGGGACT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_018966 same experiment
	EMAGE:21384 same embryo
	EMAGE:21388 same embryo
	EMAGE:21387 same embryo
	EMAGE:21385 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source