Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21426

Tspan15 tetraspanin 15 ( MGI:1917673)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426
euxassay_000759_01 euxassay_000759_02 euxassay_000759_03 euxassay_000759_04 euxassay_000759_05
EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426
euxassay_000759_06 euxassay_000759_07 euxassay_000759_08 euxassay_000759_09 euxassay_000759_10
EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426
euxassay_000759_11 euxassay_000759_12 euxassay_000759_13 euxassay_000759_14 euxassay_000759_15
EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426
euxassay_000759_16 euxassay_000759_17 euxassay_000759_18 euxassay_000759_19 euxassay_000759_20
EMAGE:21426 EMAGE:21426 EMAGE:21426 EMAGE:21426
euxassay_000759_21 euxassay_000759_22 euxassay_000759_23 euxassay_000759_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21426Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21426_wholemount_strong.wlz
21426_wholemount_moderate.wlz
21426_wholemount_weak.wlz
21426_wholemount_possible.wlz
21426_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21426_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2288
Entity Detected:Tspan15, tetraspanin 15 ( MGI:1917673)
Sequence:sense strand is shown

>T2288
GNCCTCGAGGCCAGAATTCGGATCCTTCGGTGCCAAGTCCAGAACGGACACAGCAGGCACTGGATGCTGC
CTGTGCTATCCCGATTAGCTATGCTGATTGAGCTATCCTGGCCCGGCACAGCAGCTCCCAGCCGGACTGT
ACTGCAAAGTGCATCTAAGACTACACAAGCTGGACAGGACCAGCTGCAGCTCCTCTGCCCACCCACGGCG
CTGACCAAAGCCCAGGGTGTATGTACCTGCGTATAGTGTCTGATGGCCACTCCTCCTAGGGGAAAGCTGA
ACCCTGTGGGATCCCGGGAACAGGGATAGCCCAGCTCCGGTTCTGAGTCCTGGAGAAGGCAGCTCAGGGC
TCCGTGTGGGCTCTTTTTCTTTCTGGCAGTGCCTTGGCCAGTGGTCATTATGCCCCTTCAAGGGCAGTTT
TGCAGTGATTATTTTTAAAGGCAAGAAGGGAGTGTATCTGTTCTATAGGGAAGTCCTGGGTGCAGCCCTG
GTACACTACTCTAGATGTGACGTTGGACTGTGTCTCAAATTCCCAGGTGCCTTGAGTCCTCTGTAA
Notes:The probe template was PCR amplified from IMAGE:1106451 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1106451 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000759 same experiment
 EMAGE:21423 same embryo
 EMAGE:21425 same embryo
 EMAGE:21422 same embryo
 EMAGE:21421 same embryo
 EMAGE:21424 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS