EMAGE mouse gene expression spatial database

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EMAGE:21488

Mapt microtubule-associated protein tau ( MGI:97180)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001132_01	euxassay_001132_02	euxassay_001132_03	euxassay_001132_04	euxassay_001132_05

euxassay_001132_06	euxassay_001132_07	euxassay_001132_08	euxassay_001132_09	euxassay_001132_10

euxassay_001132_11	euxassay_001132_12	euxassay_001132_13	euxassay_001132_14	euxassay_001132_15

euxassay_001132_16	euxassay_001132_17	euxassay_001132_18	euxassay_001132_19	euxassay_001132_20

euxassay_001132_21	euxassay_001132_22	euxassay_001132_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21488_wholemount_strong.wlz

21488_wholemount_moderate.wlz

21488_wholemount_weak.wlz

21488_wholemount_possible.wlz

21488_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21488_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

strong

regional

strong expression: see section 4 5 6 7 8 0 1 2 3

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 7 8 0

trigeminal v ganglion

strong

regional

strong expression: see section 4 5 6 7 8 9 0 9 0 1 2 3

vagus x ganglion

strong

regional

strong expression: see section 9 0 8 9

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 6 7 8 9 9 0

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 1 2 8

cervical ganglion

moderate

regional

moderate expression: see section 0 8

thoracic ganglion

moderate

regional

moderate expression: see section 4 5 6 7

dorsal root ganglion

strong

regional

strong expression: see section 3 4 8 9 moderate expression: see section 8 0 1 2

stomach

weak

regional

weak expression: see section 3 4 5 6 7 8 9 0 1 2 3 4

midgut

weak

regional

weak expression: see section 0 1 2 3 4 5 6 7 8 9 0 1 2

kidney calyx

weak

regional

weak expression: see section 0 1 1 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4281
Entity Detected:	Mapt, microtubule-associated protein tau ( MGI:97180)
Sequence:	sense strand is shown >T4281 CTCCAGCACCAGCAGCAGCGCCGCCGCCACCGCCCACCTTCTGCCGCCGCCGCCACAACCACCTTCTCCT CCGCTGTCCTCTTCTGTCCTCGCCTTCTGTCGATTATCAGGCTTTGAACCAGTATGGCTGACCCTCGCCA GGAGTTTGACACAATGGAAGACCATGCTGGAGATTACACTCTGCTCCAAGACCAAGAAGGAGACATGGAC CATGGCTTAAAAGCCGAAGAAGCAGGCATCGGAGACACCCCGAACCAGGAGGACCAAGCCGCTGGGCATG TGACTCAAGCTCGTGTGGCCAGCAAAGACAGGACAGGAAATGACGAGAAGAAAGCCAAGGGCGCTGATGG CAAAACCGGGGCGAAGATCGCCACACCTCGGGGAGCAGCCTCTCCGGCCCAGAAGGGCACGTCCAACGCC ACCAGGATCCCGGCCAAGACCACGCCCAGCCCTAAGACTCCTCCAGGGTCAGGTGAACCACCAAAATCCG GAGAACGAAGCGGCTACAGCAGCCCCGGCTCTCCCGGAACGCCTGGCAGTCGCTCGCGCACCCCATCCCT ACCAACACCGCCCACCCGGGAGCCCAAGAAGGTGGCAGTGGTCCGCACTCCCCCTAAGTCACCATCAGCT AGTAAGAGCCGCCTGCAGACTGCCCCTGTGCCCATGCCAGACCTAAAGAATGTAGGGTCGAAGATTGGCT CTACTGAGAACCTGAAGCACCAGCCAGGAGGTGGCAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAG CAACGTCCAGTCCAAGTGTGGCTCGAAGGATAATATCAAACACGTCCCGGGTGGAGGCAGTGTGCAAATA GTCTACAAGCCGGTGGACCTGAGCAAAGTGACCTCCAAGTGTGGCTCGTTAGGGAACATCCATCACAAGC CAGGAGGTGGCCAGGTGGAAGTAAAATCAGAGAAGCTGGACTTCAAGGACAGAGTCCAGTCGAAGATTGG CTCCTTGGATAATATCACCCACGTCCCTGGAGGAGGGAATAAGAAGATTGAAACCCACAAGCTGACCTTC AGGGAGAATGCCAAAGCCAAGACAGACCATGGAGCAGAAATTGTGTATAAGTCACCCGTGGTGTCTGGGG ACACATCTCCACGGCACCTCAGCAATGTGTCTTCCACGGGCAGCATCGACATGGTGGACTCACCACAGCT TGCCACACTAGCCGATGAAGTGTCTGCTTCCTTGGCCAAGCAGGGTTTGTGATCAGGCTCCCAGGGCAGT CAATAATCATGGAGAGAAGAGAGAGTGAGAGTGTGGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504850 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504850 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001132 same experiment
	EMAGE:21491 same embryo
	EMAGE:21487 same embryo
	EMAGE:21486 same embryo
	EMAGE:21489 same embryo
	EMAGE:21490 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source