EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21489

Slc31a2 solute carrier family 31, member 2 ( MGI:1333844)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002993_01	euxassay_002993_02	euxassay_002993_03	euxassay_002993_04	euxassay_002993_05

euxassay_002993_06	euxassay_002993_07	euxassay_002993_08	euxassay_002993_09	euxassay_002993_10

euxassay_002993_11	euxassay_002993_12	euxassay_002993_13	euxassay_002993_14	euxassay_002993_15

euxassay_002993_16	euxassay_002993_17	euxassay_002993_18	euxassay_002993_19	euxassay_002993_20

euxassay_002993_21	euxassay_002993_22	euxassay_002993_23	euxassay_002993_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21489_wholemount_strong.wlz

21489_wholemount_moderate.wlz

21489_wholemount_weak.wlz

21489_wholemount_possible.wlz

21489_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21489_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4226
Entity Detected:	Slc31a2, solute carrier family 31, member 2 ( MGI:1333844)
Sequence:	sense strand is shown >T4226 GCGACCCGGGCGGCCGTCTAGTGTGCTCACCATGCCGATGCATTTCATCTTCTCAGATGAGGCCGTGCTT CTCTTTGATTTCTGGAGGGTCCACAGCCCTACAGGCATGGCTCTCTCAGTGCTGGTAGTCTTGCTCCTGG CAGTACTGTATGAGGGCATCAAGGTTGGCAAAGCCAAATTGCTCCACAAGACTCTGGAGAGCCTGCCTGC CACCAACAGCCAGCAGTTCATCTTGGGACCAGACCAGGATTCTACAGGCTCCAGATCAACTTCAGACAAT AGGACCCGCCTCAGGTGGTTTTTGTGTTACTTTGGCCAGTCCCTAGTCCATGTCATTCAGGTGGTAATTG GCTACTTTGTGATGCTGGCTGTCATGTCCTACAACACCTGGATTTTCCTCGGTGTGGTCCTGGGCTCGGC TGTGGGCTACTACCTAGCCTACCCACTTCTCAACATGACTTAGTGGGCCAGAGATGTGCAGTGCGGAGGG CTGGAGAGAGCTGGGGCCTCTGTTCCAGACATTGTACTTCAGCTGCTTTTCCTTATGGTGGCCATTGCTC ACCTCTTTCCCAGTTCCTGGAGACTTTGAGATGAAACCAGCAAATGGTCCCTGGAGCTTAGAAGCCATTG AAGCTACCTCCCTCCCCAGCCTCTCTAGGGCCCAGGCTCCTGTGGTGCCTTAAGCAAGTAGCTGTGACCA AAGGGAACATGGAGAGACCAGAAGCTGGAGGTAGGGTGGCCAGCCTGTGACACAGATCTCTGACTGCAAA TTAAAGTTTTCCAAAGAGCTTCAGTGGTCAAGGCCATGGAACCTGGATACTCTCTTCTGCTTTGTGCCTT AATGTCAGGGTCATCTCCAGCCTTTAGGGGACAGACCACGCCAGCTCCTTGTTCTCACCTTTTTGCCTTG GAACACATGAGGATTGTCATTGACAAAACAAGCTTTTCATTTTCAACTCCTGGTTGGCAGTTTTGCACAT TCATACAAAACAAAATTTCTAATGAACTCATTGTATTCAGGATGGATGGCAGTGTCTGTTGAGACTTGTC TCCCCTTCTCGAGGGAAGACTGATGCTGATTAAAGGCAGAGACCCAGGACTGTTACTGAGGCTGTGGGCT TGTCCTGTGTACAGATGAGCTCTTCCTGTCTGACAGCACTGCCTGCAGCAGGTTAGTGGTCAGAGCCTTG CCCGAGCCTCTTGTGTGCACCAGAGAGGACAGCATCTGATAACAGAGCTGACTGCCCCTTATATTTCCTT ACTGCTAACTCCTAATGATCTGGTAGGAAATGTGAGTCAGTCACTTTAGTACTGACTCAGCAGGGGTGTG CTGGGGCTGGGTACTGACTGAGGGTGCAATAGCAATCAAACCAAAGCTAGGTCATTTTAAGAGTTTGGTG GACTCCAGATATTTGAGCCTTTTCACTTTACACAGAAAAGTTAAGACAGGAATGGGGACATATACCTGTA ATCTTCAGGATGTGGGAGGCTGAGGCAGGAGATTGCGACTTTGAGGCCAGCTTGAGCTACATAGAGAGTT GCAGGCCAGCCTGAGATACACAGTAAAACACACACACACACACACACACACACACACACACAACAATCAC TGCTACATACTTTATAATGTCTGTGTTCAAGTACCTCATGGGCCCAATGAAAAGATGGTTTTAAGAAGTT TTGGGAATTAAAAACAAATACACTTTAATAAATTTCTATTTTTGAACAGTCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502064 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502064 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002993 same experiment
	EMAGE:21491 same embryo
	EMAGE:21488 same embryo
	EMAGE:21487 same embryo
	EMAGE:21486 same embryo
	EMAGE:21490 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source