EMAGE mouse gene expression spatial database

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EMAGE:21531

Reep2 receptor accessory protein 2 ( MGI:2385070)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000999_01	euxassay_000999_02	euxassay_000999_03	euxassay_000999_04	euxassay_000999_05

euxassay_000999_06	euxassay_000999_07	euxassay_000999_08	euxassay_000999_09	euxassay_000999_10

euxassay_000999_11	euxassay_000999_12	euxassay_000999_13	euxassay_000999_14	euxassay_000999_15

euxassay_000999_16	euxassay_000999_17	euxassay_000999_18	euxassay_000999_19	euxassay_000999_20

euxassay_000999_21	euxassay_000999_22	euxassay_000999_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21531_wholemount_strong.wlz

21531_wholemount_moderate.wlz

21531_wholemount_weak.wlz

21531_wholemount_possible.wlz

21531_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21531_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 4 5 6 7 0 1

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 7 8 9

trigeminal v ganglion

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 0 8 9 0 1 2

vagus x ganglion

moderate

regional

moderate expression: see section 9 8

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 6 weak expression: see section 7 8 9 8 9

cervico-thoracic ganglion

weak

regional

weak expression: see section 1 7

cervical ganglion

weak

regional

weak expression: see section 0 7

thoracic ganglion

weak

regional

weak expression: see section 3 4 5 6 7

dorsal root ganglion

moderate

regional

moderate expression: see section 8 9 0 1 2 7 8 9

neural retina

moderate

regional

moderate expression: see section 1 2 3 3

lower jaw incisor

weak

regional

weak expression: see section 2 3 7

upper jaw incisor

weak

regional

weak expression: see section 2 3 7

testis

moderate

regional

moderate expression: see section 6 7 8 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4285
Entity Detected:	Reep2, receptor accessory protein 2 ( MGI:2385070)
Sequence:	sense strand is shown >T4285 CCCCAAAGCCGCTGCCGCTGCTAACAGCGGCTCCTGCTGCCTCAGCCGCCGGCCTCGGGCAGCTGCATCC ATACCCCGGGCTGGGTCCCCGCCCCGCCATGGTGTCCTGGATCATCTCTCGCCTGGTGGTGCTCATCTTT GGCACCCTGTACCCAGCCTATTCTTCCTACAAGGCCGTGAAGACCAAAAACGTGAAGGAATACGTAAAAT GGATGATGTATTGGATAGTCTTCGCCTTCTTCACCACAGCTGAGACACTTACAGATATAATACTGTCCTG GTTCCCCTTCTACTTTGAGCTCAAGATTGCCTTTGTGATATGGCTGTTGTCCCCTTACACCAAGGGCTCC AGTGTCCTCTACCGCAAGTTCGTGCACCCAACACTGTCCAACAAGGAAAAGGAGATCGACGAATACATCA CACAAGCTCGAGACAAGAGCTATGAGACGATGATGAGGGTGGGCAAGAGGGGCCTGAACCTGGCTGCCAA TGCTGCAGTCACAGCTGCTGCCAAGGGCCAGGGGGTGCTGTCGGAAAAGCTGCGGAGCTTCAGCATGCAG GACCTGACTCTCATTCGAGATGAGGATGCGTTACCGCTGCAGGGGCCAGATGGCCGCCTCCAACCCGGCC CCGTGGGTCTCCTGGACACTATTGAGGACTTAGGAGATGAGCCTGCCCTAAGTCTAAGGTCTAGCACAAG CCAGCCAGATCCCCGGACAGAGACCTCAGAAGATGACCTGGGAGACAAGGCACCCAAGAGGACCAAACCT ATCAAAAAAGTACCCAGAGCTGAGCCGCCGGCTTCCAAGACACTGAAGACCCGGCCCAAGAAGAAGAGTT CTGGAGGGGGCGACTCAGCATGAGAGGCTCGGCTCCCCGCACCCCTCCATCTCTAGGACAAGCACGGAGA AGAGCTGCAGCCCTGGCCCCTCCAACAGTGAGCCACTAGCCCCAAGTGTTTCAGACCCGCAAACCCCCCA GGTGGCTGTCCCCAGCACCCCCTTCCCATGAACATGCCTCCGGAGGGGGCTTGGCAAAGAGGAGGTTGAG GACAGAGGTGATGACTAAGCCCTTGAGGAGGTGGAGGCTCCGTGGCCAGCCAGCGCTGCTCTTTTTGCTG CTCCAGTCCCACGCTGCCCATCCAGTGCCTTGCTGACCATGGCCGTCCCCTCTCAGAGGCCTAACCTGCT TCCCCCACCCCCAGGGAGGGGACCTTGAGAAAGGGAAGAAACTGGGCAGGCCTGGGAGGAGGGTGTTCCT CAGGCGCAGCTATGCATGGAGACAAGGGCTTTGAATAAGCAGCACGGCCAGGCCAGCATTGCAGCTGGTA GGGCTGTGTATTCTACCAAAAGAGAGGTGAGATGCTAAGGGTGTGCGTGTTAGGGAGGTTGGGTGAGAGG GGTCACCTCCTCCCTACATCAGCTCTGGTCTTTCTTCCTCTTTATCCACCCCTGCTGGTCTCTGAACCTC AGAACTCAAGCCCTTGACAGAACGAGGCTCCCAGGGACTCCTCCCTACCCTTCCATCCTTGATCCCTTTT ACACATTCATGCCCACGTAAGCTGGACTCCTCTGCTCCTAGGCTCCATCACAGAGGGCCTGCCAAGATGG CTCCTTTCCTCTCCTGTAAAGGGGATGCATCCCACCTTGCCATGGGGTCCCCTTCCTGTGCCAAGCAAAC CCTGGTCAGAACTAACCTGGACCCCACCCCTGGCGCTGGTCCTGCCAGCCTCAATGGGGCCAAGTGGTAC CTGTGCAGGGAAGGAGATGCAGGAGCTGGGAGGCTCCCAACTCCACGGTGCCCACACTGGACCTTGGCAC TGGATGAGCCAATAAACGAAACTCTGACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4505679 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4505679 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000999 same experiment
	EMAGE:21530 same embryo
	EMAGE:21534 same embryo
	EMAGE:21529 same embryo
	EMAGE:21533 same embryo
	EMAGE:21532 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source