EMAGE mouse gene expression spatial database

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EMAGE:21533

Atp6v1g2 ATPase, H+ transporting, lysosomal V1 subunit G2 ( MGI:1913487)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001060_01	euxassay_001060_02	euxassay_001060_03	euxassay_001060_04	euxassay_001060_05

euxassay_001060_06	euxassay_001060_07	euxassay_001060_08	euxassay_001060_09	euxassay_001060_10

euxassay_001060_11	euxassay_001060_12	euxassay_001060_13	euxassay_001060_14	euxassay_001060_15

euxassay_001060_16	euxassay_001060_17	euxassay_001060_18	euxassay_001060_19	euxassay_001060_20

euxassay_001060_21	euxassay_001060_22	euxassay_001060_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21533_wholemount_strong.wlz

21533_wholemount_moderate.wlz

21533_wholemount_weak.wlz

21533_wholemount_possible.wlz

21533_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21533_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate

moderate

homogeneous

moderate expression: see section 9 0 1 4 6 7 weak expression: see section 7 8 8

midbrain lateral wall

moderate

homogeneous

moderate expression: see section 1 2 4

facial vii ganglion

weak

homogeneous

weak expression: see section 4 5 6 7 1 2

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 6 7 0

trigeminal v ganglion

weak

regional

weak expression: see section 4 5 6 7 8 9 8 9 0 1 2 3

vagus x ganglion

weak

regional

weak expression: see section 8 9 8 9

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 5 6 7 8 0 1

ventral grey horn

weak

regional

weak expression: see section 1 2 3 4 5 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4286
Entity Detected:	Atp6v1g2, ATPase, H+ transporting, lysosomal V1 subunit G2 ( MGI:1913487)
Sequence:	sense strand is shown >T4286 ATGACAGACACCCACCCGGTGCAGGAGACTGAGTGAAAACGGGAGCGCTAAGACCCAGTGGGAGTGGAGG ACCGCATCAAGGGCCCGGAGGAGTAGGCGCTGGAGGGTGGGAGCCGTCTGGTACCTCGGTCGCCTTCGGA ACAGCATCCTCTCTAGTGATAAAAATGGCCAGTCAGACCCAGGGTATCCAGCAGCTCCTCCAGGCTGAGA AGCGGGCAGCGGAGAAGGTGGCCGATGCCAGGAAGAGGAAGGCCCGGCGACTGAAGCAGGCGAAGGAGGA GGCTCAAATGGAGGTGGAGCAATACCGCAGGGAGCGGGAACAGGAGTTTCAGAGCAAGCAGCAGGCGGCC ATGGGCTCTCAGGGGAACCTGTCTGCTGAAGTGGAGCAGGCCACAAGACGGCAGGTTCAGGGCATGCAGA GTTCCCAGCAGAGGAATCGGGAGCGCGTCCTGGCTCAGCTTCTCGGCATGGTCTGTGAAGTCAGGCCCCA GGTCCACCCCAACTATCGGGTTACTGTCTAGAACCATCGCTCAGGGACACATCCCTAGAGTGACTCCTTC TGTCAGCTCCGTCCACAGAGAAATATCCCAACTCAAAACCACTTGTGTCGCATGCGCAGAGCCTTGGGTT CAATCCATAATATCATCCCCCCCACCCACCAAATCGCTTCACATAATAACCTGTTGCTGGAGGGGAGGCT TCTATGTGACAGGATCCAATATTCCCCCCTGAGACTTACAAGTAGCCTGTTCAAACCCGACAAACCCGAA TCTCTCTGTTCCATGACACCCACTGGAAGTTTACTAATGAATGCCCACGTCATCCCCACCGGACCCCTCT GTGAAATATATGTCCTGGTGACAGTTATGAAAGCACCCTGACTTCAGAGCAGGGGAACCTCTGTTCCTCT CCCACCCCTGGTCCTTGCTGGTTACACAACTGGGACAACTATTTAACCTGGAAGCCTCAGTGTCCTCATC TTTGTGACTGGAATGACAGCAACAACGCTCACTTTGTAAGGTGGCTGCTGTGAATTATCTGTGAGAATTC AGCATAGTTCCAGATGACGGAGTGCCTGTATCATGACCGCACTCTGCGTGCCTGCCAGAATTCTAACCCC CTCTCCTGACTTTTTTATTCCTTGTGTTCCAGCTTTGTTCAGCCACTGTGACACTAGTAACCCTCTAAAG AATTTGTTTCTCTGGCTCTCCCTTGGCACATCATGCCCCCGAACAGTTAAATGATAATGTTATTTAGCCC CTCTGTCCGAGTCCCAAATATATATCAAATCTCTTAGCACCTCTCTGTTTCTCCTGAGCTGGTCTTTCTC ATGGTTGGCCCCCCTCTTTCTCTGTTTTCCCAAATCTTGCTGCTTCCGGGAGCTGAAGATATCTATGACA TTCACATGCATGCATTCCTTTGTGTGCCCTCAGCACCTTGCCTCCTTGTGTAACAGCGGAGTGTCAATAA AATACTGGTGGATGAGTCAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4505711 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4505711 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001060 same experiment
	EMAGE:21530 same embryo
	EMAGE:21531 same embryo
	EMAGE:21534 same embryo
	EMAGE:21529 same embryo
	EMAGE:21532 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source