EMAGE mouse gene expression spatial database

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EMAGE:21534

Camk2a calcium/calmodulin-dependent protein kinase II alpha ( MGI:88256)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001000_01	euxassay_001000_02	euxassay_001000_03	euxassay_001000_04	euxassay_001000_05

euxassay_001000_06	euxassay_001000_07	euxassay_001000_08	euxassay_001000_09	euxassay_001000_10

euxassay_001000_11	euxassay_001000_12	euxassay_001000_13	euxassay_001000_14	euxassay_001000_15

euxassay_001000_16	euxassay_001000_17	euxassay_001000_18	euxassay_001000_19	euxassay_001000_20

euxassay_001000_21	euxassay_001000_22	euxassay_001000_23	euxassay_001000_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21534_wholemount_strong.wlz

21534_wholemount_moderate.wlz

21534_wholemount_weak.wlz

21534_wholemount_possible.wlz

21534_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21534_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 9 0 1 2 3 4

limb

weak

regional

weak expression: see section 1 2 3 4 5 6

vertebral axis musculature

weak

single cell

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 6 7 8 9 0 1 2 3 4

thymus primordium

weak

homogeneous

weak expression: see section 2 3 4 5 6

hypothalamus mantle layer

weak

regional

weak expression: see section 0 1 2 4 5 6 7

hypothalamus ventricular layer

weak

regional

weak expression: see section 2 3

thalamus mantle layer

moderate

regional

moderate expression: see section 7 weak expression: see section 8 9 0 6 7 8 9

cerebral cortex marginal layer

weak

homogeneous

weak expression: see section 1 4 5 6 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

telencephalon mantle layer

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 weak expression: see section 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

olfactory cortex marginal layer

weak

homogeneous

weak expression: see section 0 1 2 5 6 7 8

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 0 1 2 5 6 7 8

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 1 2 3 4 5 6 7 8 9 6 7 8 9 0 1 2 3 not examined expression: see section 5

heart

weak

homogeneous

weak expression: see section 7 8 9 0 1 2 3 4 5 6 7

tongue

weak

regional

weak expression: see section 1 2 3 4 5 6

tail

weak

regional

weak expression: see section 2 3 4 5 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4291
Entity Detected:	Camk2a, calcium/calmodulin-dependent protein kinase II alpha ( MGI:88256)
Sequence:	sense strand is shown >T4291 GTCAAGCCGGTTCTCCGTTTGCACTCAGGAGCACGGGCAGGCGAGTGGCCCCTAGTTCTGGGGGCAGCGC TTCAGCATCCCAGCCCTAGTTCCCAGCCTAAAGCCTCGCCTGCCTGCCCAGTGCCAGGATGGCTACCATC ACCTGCACCCGATTCACAGAAGAGTACCAGCTCTTTGAGGAACTGGGAAAGGGAGCCTTCTCTGTGGTGC GCAGGTGTGTGAAGGTGCTGGCTGGTCAGGAGTATGCTGCCAAGATTATCAACACCAAGAAGCTTTCAGC CAGAGATCACCAGAAGCTGGAGCGTGAGGCCCGCATCTGCCGCTTGTTGAAGCACCCCAATATCGTCCGA CTCCATGACAGCATCTCCGAGGAGGGGCACCACTACCTTATCTTCGATCTGGTTACTGGTGGGGAACTGT TTGAAGACATTGTGGCCCGGGAGTATTACAGTGAAGCTGATGCCAGCCACTGTATCCAGCAGATCTTGGA GGCTGTGCTACACTGTCACCAGATGGGGGTGGTGCATCGTGACCTGAAGCCTGAGAATCTGTTGCTGGCC TCGAAGCTCAAGGGCGCTGCTGTGAAGCTGGCAGACTTTGGCCTGGCCATAGAGGTGGAGGGGGAGCAGC AGGCATGGTTTGGGTTCGCAGGGACACCTGGATACCTCTCCCCAGAAGTGCTGAGGAAGGACCCGTACGG GAAGCCCGTGGACCTGTGGGCCTGTGGCGTCATCCTGTATATCTTGCTGGTTGGGTATCCCCCGTTCTGG GATGAAGACCAGCATCGCCTGTACCAGCAGATCAAAGCTGGTGCCTATGATTTCCCATCACCAGAATGGG ACACCGTCACCCCAGAAGCCAAGGATCTGATCAATAAGATGCTGACCATCAACCCGTCCAAACGCATCAC GGCCGCTGAGGCTCTCAAGCACCCATGGATCTCGCACCGCTCCACCGTGGCCTCCTGCATGCACAGACAG GAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAGGGAGCCATCCTCACCACTATGC TGGCCACCAGGAACTTCTCCGGAGGGAAGAGCGGAGGAAACAAGAAGAACGATGGTGTGAAGGAATCTTC TGAGAGCACCAACACCACCATTGAGGACGAAGACACCAAAGTGCGCAAACAGGAAATTATCAAAGTGACA GAGCAGCTGATCGAAGCCATAAGCAATGGAGACTTTGAGTCCTACACGAAGATGTGCGACCCTGGAATGA CAGCCTTTGAACCAGAGGCCCTGGGGAACCTGGTGGAGGGCCTGGACTTTCATCGATTCTATTTTGAAAA CCTGTGGTCCCGGAACAGCAAGCCCGTGCACACCACCATCCTGAACCCTCACATCCACCTGATGGGTGAC GAGTCAGCCTGCATCGCCTATATCCGCATCACTCAGTACCTGGATGCAGGCGGCATACCCCGCACGGCCC AGTCAGAGGAGACCCGCGTCTGGCACCGCAGGGACGGCAAATGGCAGATCGTCCACTTCCACAGATCTGG GGCGCCCTCCGTCCTGCCGCATTGAAGGACCAGGCCAGGGTCCCTGCGTCCTTGCTTCGCAGAGATCCGC TCTTTGTCCGTGGAATGTGGCTGCTGGTTCTCCTTTGGATTTTGCTGGAATTCTCCCTGTCAGATCACCC TACCATTGCCACCTATGTACTCGCGTCACGAAAACCTGCTTGTTCACAGAAGTCGCCACGACATCACAGT GAACAGCCAGCTCTCCCCAGCTCCGTTGCCCAAGCTCTTCCTGCCAGTGGGGACCTTCTTCCGGCTTAAG TACCCAGGGTGCTGGCCCCAGGAACCCCCACCCCCTACCCACTGTTGTTGGCCTAGCCTAGCTTTAGCTA TAGATGGGGCCTCAGCTGTGCAATTGGCAGGAAGTGAGGAAGAGGCAGGCAAGCTGTGTTGAGGGCACCT CTCATCGATTCCTTCTTTCCTGGGGTTCCCCGGGGAAGCTCACACGAGGCCCTCAGTCTCCAAGCCAACC CCTTATGAGGGAGAGTGAGAGAGGAGCCAACGCCAGTGAGCCAGGAACTGCTGCTCTCATCTGCTCTCCT CTGTGTTGGCCTTGCCTTTGACCAGACCATCCGCTACGAGGGGTGGGCTCTACCGCCCAGGTGCCCCACT CACTCTGCCTCAGTCCTCCTGTGAAGTTTGCCTCCAGTGTTGACCCACCCACCCTGCCCTTCAACGTCCT TGGAGAATTCCAGCTTCATCTGTCTGAGAGGAGATTGGAAGGTGTTTCAGGGGCAAAGCAAGCAACATTT AGGTATCACTTCTACTTGGACGCATGCCTTTTTACAGCCAAACTTCCGTGTATTTCGTAAATGGATTTTG CGTTAACGGACATCTATGTGATAACTAGACCTCTCAAGTTTACTGTAAAGAAGAGTCGGATGGGTTGGGA AGTGGGTGGGAAGAGGAGTGAGAGGAAGTTTTAACCCCATTCCAGAGGGTCTTTTTTTTGGGGGGTGTCC CTTCTGGGGGAGGGTGCTTTCTGAGGTGGCCTCACCCCCCAGGGAGCATGGTTTCCTTCCATTATGGTCC CCAAAGTCAGCTGACAAGATTTCTTCCAGAGCCAGCATGACTAACACACAGTGAGTCAGGTCAGGGGAGG CTATCAGGAAGGGGTTATCTAGACTTGGCATCTCTGGAACTGGAGACCTCACTACCCTACTTCCCAGGAA ATCTTTCCCGTATCCTTGTGCTATCTCTCCTCAGCCCAGCCCCCACTCACCACCTTCTAGGCACATTATT CTCATCACCTCTCTCAACTTGGCCAAGCAAGAGTGCCAGAAACTTATTTTCCTACTTCATGCTGAACTTG GCCTGGTGTGCGCTCTATTGTGCTTGCCTGCCTCCCTGGCCTTCCTCGACTCCCCTGCCCCGACATACAC ACACACACACACACACACACACACACGCACACGCACACGCGCGCACACACACACATGCACACGCACATGC ACACACTCACACCACTTCCTTCCACCACTTCCTTCCTCCCCCCTCTCCCTCCCTTTCTCTTTCTCTTCTC TCCCTCTTCCTGGGTTTGGCTCTTGTATGGAATGCTGTATCTCATTCACGGGGATCTCCTGTCTGCACTG TTTTCTTTGCATGACTTTATATGCAGTAAGTATGTTGAAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4506005 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506005 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001000 same experiment
	EMAGE:21530 same embryo
	EMAGE:21531 same embryo
	EMAGE:21529 same embryo
	EMAGE:21533 same embryo
	EMAGE:21532 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source