EMAGE mouse gene expression spatial database

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EMAGE:21536

Kcnab2 potassium voltage-gated channel, shaker-related subfamily, beta member 2 ( MGI:109239)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001129. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001129_01	euxassay_001129_02	euxassay_001129_03	euxassay_001129_04

euxassay_001129_05	euxassay_001129_06	euxassay_001129_07	euxassay_001129_08	euxassay_001129_09

euxassay_001129_10	euxassay_001129_11	euxassay_001129_12	euxassay_001129_13	euxassay_001129_14

euxassay_001129_15	euxassay_001129_16	euxassay_001129_17	euxassay_001129_18	euxassay_001129_19

euxassay_001129_20	euxassay_001129_21	euxassay_001129_22	euxassay_001129_23	euxassay_001129_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21536_wholemount_strong.wlz

21536_wholemount_moderate.wlz

21536_wholemount_weak.wlz

21536_wholemount_possible.wlz

21536_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21536_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6

pons

moderate

regional

moderate expression: see section 6 7 8 9

facial vii ganglion

strong

regional

strong expression: see section 3 4 5 0 1

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 7 8 9

trigeminal v ganglion

strong

regional

strong expression: see section 2 3 4 5 6 7 8 7 8 9 0 1 2

dorsal root ganglion

strong

regional

strong expression: see section 8 9 0 1 6 7 8 9

nasal capsule

weak

regional

weak expression: see section 9 0 3 4 5 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4243
Entity Detected:	Kcnab2, potassium voltage-gated channel, shaker-related subfamily, beta member 2 ( MGI:109239)
Sequence:	sense strand is shown >T4243 GGGGGTGCTAAAGTCGCAAGGGCAGGGCAGGAAGGAAGATCCGGAACGGGGCGTGGGATCCGGAGCTGGA GGCCCCCAGGATTCCCCCTCTGTGAAAAGGCACGCTGATCTGTCTGGATAAGTGTGGCCGGCCCCATGTA TCCGGAATCAACCACGGGGTCCCCAGCTCGACTCTCCCTGCGGCAGACAGGCTCCCCCGGGATGATCTAC AGTACTCGTTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAATCTGGGCAAATCTGGCCTTCGGG TCTCCTGCCTGGGGCTTGGAACATGGGTGACCTTCGGGGGCCAGATCACGGATGAGATGGCAGAGCACCT AATGACCTTGGCCTACGATAATGGCATCAACCTGTTCGATACGGCGGAGGTCTACGCTGCTGGAAAAGCT GAAGTGGTATTAGGGAACATCATTAAGAAGAAGGGATGGAGACGGTCCAGCCTTGTCATCACCACCAAGA TCTTCTGGGGTGGAAAAGCGGAGACTGAGAGAGGCCTTTCCAGGAAGCACATAATTGAAGGACTGAAAGC GTCCCTGGAGCGGCTGCAGCTGGAGTACGTGGATGTGGTTTTTGCCAACCGCCCAGACCCCAACACGCCC ATGGAAGAGACCGTGCGGGCCATGACCCATGTCATCAACCAGGGGATGGCCATGTACTGGGGCACATCAC GCTGGAGCTCCATGGAGATCATGGAGGCCTACTCGGTGGCTCGGCAGTTCAACCTGATCCCGCCCATCTG CGAGCAAGCGGAATATCACATGTTCCAGAGGGAGAAGGTGGAGGTCCAGCTGCCAGAGCTGTTCCACAAG ATAGGAGTAGGTGCCATGACCTGGTCCCCTCTGGCGTGCGGCATCGTCTCAGGGAAGTATGACAGCGGGA TCCCACCCTACTCCAGAGCCTCCCTGAAGGGCTACCAGTGGTTGAAGGACAAGATCCTGAGTGAGGAGGG TCGCCGCCAGCAGGCCAAGCTGAAGGAACTGCAGGCCATTGCCGAACGCCTGGGCTGCACCCTACCCCAG CTGGCCATAGCCTGGTGCCTGAGGAATGAGGGTGTCAGCTCCGTGCTTCTGGGTGCTTCCAATGCAGAAC AACTTATGGAGAACATTGGAGCAATACAGGTCCTTCCAAAATTGTCGTCTTCCATCGTCCACGAGATCGA CAGCATTCTGGGCAATAAACCCTACAGCAAAAAGGACTATAGATCCTAAGTCTACTCCCCACCCCTCGTG CCCCACCCGGACAGTTCCCGGTTCCCTCCTCGTCTCTGTTCGCTCGCTTCTGCTGTTCCAAAGCCAAATG CAGAGTGTGGTTCACATCCAAAGGGAAATCACTATGCCCAGACGTGACAGGGAATCGACCTCCAAAGTCA CCGCCAGATGCCGCCTGCCGCTTCTTCACTGGATACTGTCCGACAACGGATGCCAGGGGATGGCATCAGT TAGGGGGACATCCAGAGGCTCCCGCCCAAGCCCACCACCTCTGCTTATCCTTCAAGAAGAAACACTTGCC CCCAGCCATGGCCCTTCACGGAAATTCTAGAAGCCATGGCCGGGTGGAGGTCTCATCCTTAGAGCAGGAG GGGCCGCCAAGAGCCCATCTCAAAAGAGGGGTGTGCACCCTTCTCCCAGAGTCCTGGTGGTTAGAGCAGG GCCCTCCCTCTTTCCATGGACACCAGGTACTTTGCTCAAGGCTGGCCTGGATCCCATTCTTCCTAGGTCT TGACCTGCCCCACATACCCACCGAGCCTTGGCTGACATAGCTACCCAGCTGGCCCTTGGCCCCCACCCTC CCCCTTGCTGGCAGGGCCAGGGATTCCAGCTGGCTTTCCCAGCATCTTCCAACTGCTGAGCCCCACAGCA GGCAAGCTCAGCAGGCTTGGATGGAGCCCATCGGAGTATGCCTCTCAAGACAGACACTCGTGGGCAAGTG TGGCAGCTGGCCCCACCGCAGGCAGGTGGCAGCAGGCTTGCAAGACCATATTGCTCTCACAGACCTTCCA ATACCTCTGCTGTACCGGGGCAGTGGGCTCATGATGTGGGCTTTGCCCAGATGAAGCACTTAGAGCTATG TTGCAAGCCAGGGAACTACGGGAGCAGCTCCAGTAATAGTCTCCGTGTTTTAAGAATAGATCCCAGAGCT TCTCCCTGGAAAAATGGCTAGGACCACCCATTCCCTTCTGCGTACCCTCATGCTGGCCTAGGGCAGGATG GGGAGAGGAAGTAGTGGAAAGGATAGGCAGCCCTTTATCACTGGGATAAATCCTGCATGGATGTTGATGC CAGGGAGCCTGCGGGATTGGTCAGTACACACTGCCTGGAGGCCCCATCCCATGGTTTTGTGATGCTCTGG GGGACAGGGTCCTACCCAGCTGCCTCCCTAGGAGACCAGCCTCCTGCCCTGCTGCACAGACCATGGCCAG CTCCCAGGCACAAGTCCTAGATGCTTAGCACCCTAGCTTTGCCTTCTGTAGCTGCTATTACAAGGCTGCT CCTTGCTGCTCTTAAGGCTGGGCAGAGGTGAGGACAGGGGTGGGGGTTGGGGGGGTTTATGGGGAGGAGA CAGGAAGAAAGGCAGACAAACCCCCCCCCGAAAACCCTGAGCTCTCTAACCTTCGCTGCTCATAGTGCTG ACCCTAAGACCTCAGAAGCAGCTCTGTGTGTGGGGGGATGGAGACCATGGGCTCTGGTATTTCCATGCCT GGCTAGCAGAGCCAGCTTTCTGGACAGGGCCCTGCAGTGGCCCAGCAGGAAGTACCAGACCTGTCCACAC AGCTGGCCTTGGCCCTGCTTGTTAGGTGGGCCAGGCATGCAGGGATGCCCACAGTGGGAGCTACTGCCTG CCTGTGAACCACACCTCAGAGCTAAGCTCCTGCTGGTGATGTGCAAGAGACAGGAAAACAGGCTGCCGGG CTAAGGCCCATAGGATTTAGCCCACAGGAGCCCTAGATGCAAAAGACATGTTAACCAAGTGTTTCTGAGA GGTTGTCTTCCACACCCACTCTCGAAGCGTCAGGGATTTGCTTCTTAAACTTCTGCGGAGGGGTTTGGCT TGGGAGGCCATGTAGAGCAAGTACAGACAGCCTGTTAGTGGGAGCTTGCCCCCAGGCCCTCAAAGGGTGT AAGGCCAGCTCCTTCCTCCATGGCCTCTCCTTCCTTTAGCCCAGCTGCCTGCAGGCCAGCCCAAGCCAGC TTCTCTTCACCTTGAAGTAGTCTGCCAGGCCTGGTGCAGGAGGAGGGCCATGTAGAGAGAGGATCTGGCA CTCGGAGCCCAAGGTCCCAAGGCACAGCAGCGCACCTGTGTGCCTGCTCTCCTCATGTACATACATAACG TGCTTTCTCTCCCCGTTTTTTGTTTGTTTTTTTTTAAACGAGCGTGGCTCCTCGCTAACTGCACCCCACC AGGCCGACAGCAAACGCCTCCAGCTTCCCGACAGACTCAGACCAAGGTGCGGCCCCGTATTTATGGAATG GCAAATAAAACCCGAGCCCTTTGGTCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:4503373 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503373 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001129 same experiment
	EMAGE:21535 same embryo
	EMAGE:21538 same embryo
	EMAGE:21539 same embryo
	EMAGE:21537 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source