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EMAGE:21537

Nefl neurofilament, light polypeptide ( MGI:97313)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21537 EMAGE:21537 EMAGE:21537 EMAGE:21537 EMAGE:21537
euxassay_001137_01 euxassay_001137_02 euxassay_001137_03 euxassay_001137_04 euxassay_001137_05
EMAGE:21537 EMAGE:21537 EMAGE:21537 EMAGE:21537 EMAGE:21537
euxassay_001137_06 euxassay_001137_07 euxassay_001137_08 euxassay_001137_09 euxassay_001137_10
EMAGE:21537 EMAGE:21537 EMAGE:21537 EMAGE:21537 EMAGE:21537
euxassay_001137_11 euxassay_001137_12 euxassay_001137_13 euxassay_001137_14 euxassay_001137_15
EMAGE:21537 EMAGE:21537 EMAGE:21537 EMAGE:21537 EMAGE:21537
euxassay_001137_16 euxassay_001137_17 euxassay_001137_18 euxassay_001137_19 euxassay_001137_20
EMAGE:21537 EMAGE:21537 EMAGE:21537
euxassay_001137_21 euxassay_001137_22 euxassay_001137_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21537Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21537_wholemount_strong.wlz
21537_wholemount_moderate.wlz
21537_wholemount_weak.wlz
21537_wholemount_possible.wlz
21537_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21537_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
limb
moderate moderate
regionalmoderate expression: see section 1 2 3 4 2 3
vertebral axis musculature
weak weak
regionalweak expression: see section 7 8 9 0 1 2 3 4 5 6 8 9 0
adrenal gland
moderate moderate
homogeneousmoderate expression: see section 0 1 3 4 5 6 weak expression: see section 9
foregut-midgut junction
weak weak
regionalweak expression: see section 0 1 2 3 4 5
hypothalamus mantle layer
weak weak
homogeneousweak expression: see section 7 8 9 0 1 3 4 5 6
diencephalon lateral wall mantle layer
weak weak
regionalweak expression: see section 8 9 0 1 2 3 4 5 6
thalamus mantle layer
weak weak
homogeneousweak expression: see section 7 8 9 0 1 4 5 6 7
cerebral cortex marginal layer
weak weak
homogeneousweak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 1 4 weak expression: see section 2 3 4 5 6 7 8 9 0 2 3 5 6 7 8 9 0 1 2
olfactory cortex marginal layer
weak weak
regionalweak expression: see section 8 9 0 4 5 6
hindbrain
moderate moderate
homogeneousmoderate expression: see section 4 weak expression: see section 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1
metencephalon basal plate
moderate moderate
regionalmoderate expression: see section 6 7 8 9 0 1 2 3 4 5 6 7 8
midbrain mantle layer
moderate moderate
homogeneousmoderate expression: see section 1 2 3 4 5 6 weak expression: see section 7 8 9 0 7 8 9
midbrain marginal layer
moderate moderate
homogeneousmoderate expression: see section 0 1 2 3 4 5 6
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 4 5 6 9 0 1
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 6 7 8 8 9
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 2 3 4 5 6 7 8 7 8 9 0 1 2
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 8 9 7
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 6 7 8 7 8
spinal cord
weak weak
homogeneousweak expression: see section 1 2 3 4 5 6 7
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 8 9 0 1 2 3 4 6 7 8
anal canal
moderate moderate
regionalmoderate expression: see section 9 0 1 2 6 7 8
tongue
weak weak
regionalweak expression: see section 1 2 3
stomach
weak weak
regionalweak expression: see section 3 4 5 6 7 8 9
midgut
weak weak
regionalweak expression: see section 8 9 0 1 2 3 4 5 6 7 8
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 9
lower jaw molar
moderate moderate
regionalmoderate expression: see section 0 4
oral epithelium
moderate moderate
regionalmoderate expression: see section 6 weak expression: see section 8 9 0 9 0
maxilla
moderate moderate
regionalmoderate expression: see section 1 3
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 4 5 6 7 1 2 3 7 8
upper jaw molar
moderate moderate
regionalmoderate expression: see section 4 9 0 3 4 5 9
bladder
moderate moderate
regionalmoderate expression: see section 9 0 1 2 3 4 5 6 7
temporo-mandibular joint primordium
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 9 0 1 2 3
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4307
Entity Detected:Nefl, neurofilament, light polypeptide ( MGI:97313)
Sequence:sense strand is shown

>T4307
CCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGGTCCCCCATCTCCGCCCCGGGCCTGAGGGGCGCACCG
GCCGCCACCATGAGTTCGTTCGGCTACGATCCGTACTTTTCGACCTCCTACAAGCGGCGCTATGTGGAGA
CGCCCCGGGTGCACATCTCCAGCGTGCGCAGCGGCTACAGCACGGCGCGCTCCGCGTACTCCAGCTACTC
CGCGCCGGTCTCCTCCTCGCTGTCCGTGCGCCGCAGCTACTCGTCCAGCTCTGGCTCTTTGATGCCCAGC
CTGGAGAATCTCGATCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCTATCCGCACACAAGAGA
AGGCACAGCTGCAGGACCTCAACGATCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCA
GAACAAGGTCCTGGAAGCCGAGCTGTTGGTGCTGCGCCAGAAACACTCTGAGCCTTCCCGCTTCCGCGCC
CTGTACGAGCAGGAGATCCGCGATCTGCGGCTGGCAGCGGAAGACGCCACTAACGAGAAGCAGGCGCTGC
AGGGCGAGCGCGAGGGGCTGGAGGAGACTCTGCGCAACCTGCAGGCTCGCTATGAGGAAGAAGTGCTGAG
CCGCGAGGACGCCGAGGGCCGGCTGATGGAAGCGCGCAAAGGTGCGGATGAGGCCGCGCTCGCCCGCGCC
GAGCTGGAGAAGCGCATCGACAGCCTGATGGACGAGATAGCTTTCCTGAAGAAGGTGCACGAGGAAGAGA
TCGCCGAGCTGCAGGCTCAGATCCAGTATGCTCAGATCTCCGTGGAGATGGACGTGTCCTCCAAGCCCGA
CCTCTCCGCCGCTCTCAAGGACATCCGCGCTCAGTACGAGAAGCTGGCCGCCAAGAACATGCAGAACGCC
GAAGAGTGGTTCAAGAGCCGCTTCACCGTGCTAACCGAGAGCGCCGCCAAGAACACCGACGCTGTGCGCG
CTGCCAAGGACGAGGTGTCGGAAAGCCGCCGCCTGCTCAAGGCTAAGACCCTGGAGATCGAAGCCTGCCG
GGGTATGAACGAAGCTCTGGAGAAGCAGCTGCAGGAGCTAGAGGACAAGCAGAATGCAGACATTAGCGCC
ATGCAGGACACAATCAACAAACTGGAGAATGAGCTGAGAAGCACGAAGAGCGAGATGGCCAGGTACCTGA
AGGAGTACCAGGACCTCCTCAATGTCAAGATGGCCTTGGACATCGAGATTGCAGCTTACAGAAAACTCTT
GGAAGGCGAAGAGACCAGGCTCAGTTTCACCAGCGTGGGTAGCATAACCAGCGGCTACTCTCAGAGCTCG
CAGGTCTTCGGCCGTTCTGCTTACAGTGGCTTGCAGAGCAGCTCCTACTTGATGTCTGCTCGCTCTTTCC
CAGCCTACTATACCAGCCACGTCCAGGAAGAGCAGACAGAGGTCGAGGAGACCATTGAGGCTACGAAAGC
TGAGGAGGCCAAGGATGAGCCCCCCTCTGAAGGAGAAGCAGAAGAGGAGGAGAAGGAGAAAGAGGAGGGA
GAGGAAGAGGAAGGCGCTGAGGAGGAAGAAGCTGCCAAGGATGAGTCTGAAGACACAAAAGAAGAAGAAG
AAGGTGGTGAGGGTGAGGAGGAAGACACCAAAGAATCTGAAGAGGAAGAGAAGAAAGAGGAGAGTGCTGG
AGAGGAGCAGGTGGCTAAGAAGAAAGATTGAGCCCTATTCCCAACTATTCCAGGAAAAGTTCTCCCCAAT
CAGGTCAACCTCATCACCAACCAACCAGTTGAGTTCCAGATCCTATACAAATTAAGAAGTCAATACATGT
ATAATTCTGAGATGACTTAGGTTGGACTTTCAATGTTGTGCTATGAATTTCCTCCTTACGCAGAGTATCT
GTTTGCTTGCAGAGTGGCTTTCTGGCTTGCTGCCAGCCTGTGCATGGTCCATGCTTATGAGTTCAGGATC
TATGGCAATGTGAATCACACAGATGTTTACAATAATAATAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4506903 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4506903 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001137 same experiment
 EMAGE:21535 same embryo
 EMAGE:21538 same embryo
 EMAGE:21539 same embryo
 EMAGE:21536 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS