EMAGE mouse gene expression spatial database

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EMAGE:21539

Chrna6 cholinergic receptor, nicotinic, alpha polypeptide 6 ( MGI:106213)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001125. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001125_01	euxassay_001125_02	euxassay_001125_03	euxassay_001125_04

euxassay_001125_05	euxassay_001125_06	euxassay_001125_07	euxassay_001125_08	euxassay_001125_09

euxassay_001125_10	euxassay_001125_11	euxassay_001125_12	euxassay_001125_13	euxassay_001125_14

euxassay_001125_15	euxassay_001125_16	euxassay_001125_17	euxassay_001125_18	euxassay_001125_19

euxassay_001125_20	euxassay_001125_21	euxassay_001125_22	euxassay_001125_23	euxassay_001125_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21539_wholemount_strong.wlz

21539_wholemount_moderate.wlz

21539_wholemount_weak.wlz

21539_wholemount_possible.wlz

21539_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21539_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon floor plate

moderate

regional

moderate expression: see section 1 2 3 5 6 7 8 weak expression: see section 9 0

midbrain floor plate

moderate

regional

moderate expression: see section 1 6

midbrain lateral wall

moderate

regional

moderate expression: see section 2 3 4 5

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4216
Entity Detected:	Chrna6, cholinergic receptor, nicotinic, alpha polypeptide 6 ( MGI:106213)
Sequence:	sense strand is shown >T4216 GACAGGACATGTGACAGCCAGCCGAGTGAAGCAGCAGGGTTGGCTTCCCTTGCCCTTCACATCTGTTTCA GCCTGGGATGCAGGGAGCAGTTGCATGATGTCCCGTGTTTTCACTTCACAGAAGATCTGTGTGAGCTTCC AGGAGCTTCCCGGGGCTTTCAATATCTGATTCTGGCAGCTGACAGACAGCTGTGTCTTCCACAGCCCTCC GTGTAGAAATCGGAATTCAGAGAGCTTTTATCATGCTGAACAGCCGGGACCAGGGAAACCTGCACTCCGG TTTATGTCTGTGGCTATGTGGATTCCTGGCTCTCTTTAAAGGCAGTACAGGCTGTGAATCTGAAGAGCAG CTCTTCCACAGGCTGTTTGCTCACTACAACCGCTTCATCCGGCCGGTGGAGAATGTCTCCGATCCCGTCA CGGTGCATTTTGAATTGGCAATCACGCAACTGGCCAATGTGGATGAAGTCAACCAGATTATGGAGACCAA TCTGTGGCTGCGTCACATCTGGAAGGACTACAGATTGCGTTGGGATCCAACGGAGTATGATGGCATCGAG ACACTTCGAGTTCCAGCAGACAACATCTGGAAGCCTGACATCGTTCTGTATAACAATGCTGTCGGCGACT TCCAGGTCGAAGGCAAGACCAAAGCTCTTCTCAAGTATGACGGTGTGATAACCTGGACCCCACCAGCCAT CTTTAAGAGCTCCTGCCCAATGGACATCACCTTCTTCCCGTTTGACCATCAAAACTGCTCCCTGAAGTTT GGTTCCTGGACTTATGACAAGGCAGAAATCGACCTTCTCATCATTGGCTCTAAAGTAGACATGAACGACT TTTGGGAAAACAGTGAATGGGAAATTGTCGACGCCTCTGGCTATAAGCATGACATCAAGTACAACTGCTG TGAAGAGATTTACACGGACATAACCTACTCCTTCTACATCAGGAGGTTGCCCATGTTTTACACCATCAAC CTCATCATCCCCTGCCTCTTCATTTCCTTCCTCACGGTGCTGGTTTTTTACCTTCCCTCCGACTGTGGCG AGAAAGTGACTCTTTGCATCTCCGTTCTGCTTTCTCTCACTGTCTTTTTGCTGGTGATTACAGAGACCAT CCCATCCACATCTCTCGTGATCCCACTGGTGGGTGAGTATCTACTGTTCACCATGATCTTTGTCACGCTG TCCATTGTGGTGACCGTGTTCGTGCTGAACATACACTACAGGACCCCAGCAACGCATACCATGCCCAAGT GGGTGAAGACCATCTTCCTTCAGGCCTTCCCCTCGATTCTGATGATGAGGAAACCTCTGGACAAGACAAA GGAGGCAGGAGGTGTTAAGGACCCCAAAAGCCATACCAAGAGGCCTGCCAAGGTCAAATTTACTCATCGA GGAGAATCCAAACTTCTAAAGGAATGCCACCACTGCCAAAAATCAAGTGACATAGCACCTGGAAAGAGAA GATCAAGCCAGCAGCCTGCACGGTGGGTGGCAGAGAATTCAGAGCACTCGTCCGATGTTGAAGATGTCAT CGAGAGTGTTCAATTCATAGCAGAAAACATGAAGAGCCACAATGAAACAAACGAGGTAGAAGACGACTGG AAATACATGGCTATGGTGGTGGACAGAGTCTTCCTTTGGGTATTTATAATTGTCTGTGTGTTTGGAACTG TGGGGCTATTTCTGCAGCCACTGCTTGGGAACACAGGAAAGTCTTAATTGGTATTGTCCCTTTACCTTCT GAATCTTTTAGATGTTGTGTTTTTTCCCGAGCTAGTCCCCACAAGAAAAAGCCAAGTACACAGACTCCTA AACCAAGACAAGAAGGGAGTGGGCATTGCTGCAGCCTTAGGAGCCTGAATACCTGTCTTCCTTACACTGG GTGTTTTATCACCTTGGAGCCCAGAGCTGGCAGTTGTCCTAGGCTAGGAGGACTGAGTCAGCTTCTCTAT AATTTGCATTCTTTGTTTCAAGAAACACAAATGGTCCCTCGTAGAGCTAGGCCCCATAGTCAAGAGTCAG TCCACTCTGAAAGGTGATTGGCTTGTATCCGAAGCTCCTGCTGGTTATTGCAAGTGATGTGTATGGTTAT CAAGAAACTCGGGAGCGCTGCTGACACTTGCAACACACCTGTCTGCTACTTTCTTGTGCATGTCCCTGTG ATGTAGCTCACACTCATATCTGACATGTGCAATGGTCAAAGAAGAAAGCAGATAGCATCTCTCTTAGCAG ACTATTCAATTCGTGGTGCAATGGGTTTTCTGTCCGTGTGAAAATCACTGAACTGATAGGCGTAAAAGGA TGACGTTTATATGAGCTCATGGATTTGGAGGACTCTGCCCATGATCACTAGGTCCCATTGCCTCTGGGCC TTTGATGAGGCAAAGCATCATGCCTAGAAGAGGATGGCTGCCCACTTAAAGATAGCTACAAAGAAAGCAA ACAGAGAGGAAGGCTCTGGAGTGCCAATATTCCTTTCACAGTCACAGCCCAATGACTTCATGTCTTCCTA CAAGACCTTCCATCCTAGAAGCTCCACTACCTCCTGATAGCGTGGAGGAATCAGTCTCTAACACTTGTCC TTGGGGCTGATCCAAACCACAGCAGTATTGTCCTGGCAGGACATTTGGCTGGCTCCAGTACTGTGCTAGG TGCTACAAGGGGATACATAAGAGTCTTTCCCTTATAAAGTTACAGCCTTTTAGAAAAGCCGTATATGCAA AGAGCCACAGGATCAGGTAGTAGAGGATATAGCTAGAGCAACCCAAATGGGAGAAACTAATCGGACAAAC CTAAACTCACTCTTGTGTTTCAAACAGATATCTGTGTAGCTTTTTCCCACGAGCCCTTCTCTAGCTCACT GCATGGAGATGAAATAAAATCGATATGAAATTTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4501558 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501558 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001125 same experiment
	EMAGE:21535 same embryo
	EMAGE:21538 same embryo
	EMAGE:21536 same embryo
	EMAGE:21537 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source