EMAGE mouse gene expression spatial database

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EMAGE:21540

Fam83f family with sequence similarity 83, member F ( MGI:2146227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001007. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001007_01	euxassay_001007_02	euxassay_001007_03	euxassay_001007_04

euxassay_001007_05	euxassay_001007_06	euxassay_001007_07	euxassay_001007_08	euxassay_001007_09

euxassay_001007_10	euxassay_001007_11	euxassay_001007_12	euxassay_001007_13	euxassay_001007_14

euxassay_001007_15	euxassay_001007_16	euxassay_001007_17	euxassay_001007_18	euxassay_001007_19

euxassay_001007_20	euxassay_001007_21	euxassay_001007_22	euxassay_001007_23	euxassay_001007_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21540_wholemount_strong.wlz

21540_wholemount_moderate.wlz

21540_wholemount_weak.wlz

21540_wholemount_possible.wlz

21540_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21540_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 7 8 9 0 1 8 9 0 1 2

foregut-midgut junction

weak

regional

weak expression: see section 6 7 8 9 not examined expression: see section 0

pancreas

weak

regional

weak expression: see section 1 2 3 4

vibrissa

weak

regional

weak expression: see section 5 6 7 8 9 1 2 3 4

pharyngo-tympanic tube

weak

regional

weak expression: see section 1 2 3 4

pharynx

weak

regional

weak expression: see section 6 7 8 9 0 1 2 3 4 6 7 8 9 0 1 2

esophagus

weak

regional

weak expression: see section 3 4 5

stomach

weak

regional

weak expression: see section 3 4 5 6 7 8 9

hindgut

weak

regional

weak expression: see section 6 7 not examined expression: see section 8

midgut

weak

regional

weak expression: see section 3 4 8 9 0 not examined expression: see section 7

lower jaw incisor

weak

regional

weak expression: see section 2 3 7 8

lower jaw molar

weak

regional

weak expression: see section 8 0 1

oral epithelium

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9

upper jaw incisor

weak

regional

weak expression: see section 2 3 7 8

upper jaw molar

weak

regional

weak expression: see section 8 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T320
Entity Detected:	Fam83f, family with sequence similarity 83, member F ( MGI:2146227)
Sequence:	sense strand is shown >T320 GGGACAGCAGGGTCCGAGCCTGGGGCCCCGGGACAGGGCTGGGGCGCCATGGCCGAGTCCCAACTGAGCT GCCTGGACGAGGCGCACGTGAACGAGAGGGTGACCGAGGCACATGCAGCTTTCTACTACTGCGAGAGGCG GCGGGCCGCGCTGGAGGCGCTGCTGGGTGGAGGCGAGCAGGCCTACCGCGAACTGGTCAAGAAGGAGCGG CTGCGGGACTTCCTCTCCAGCCAGGAGCGCCAGGCCCTCTGCGCCGCCTGGAGCCCCTACGAGGAGGCAG TCTCCAGCACCCGTGCCAAGGCTAAGGCCAAGGCCAAGGTCCCGGCCCAGCCCACTGAGTCCCTGGCCTA CTGGCCAGACCGCTCGGACACCGAAGTGCCCCCACTGGACCTGGGGTGGACAGACACCAACTTCTACCGC GGTGTGAGCCGAGTCACGCTCTTCACCCACCCGCCCAAAGAAGAGAAGGCACCGCACCTGAAGCAGGTGG TCAGGCAGATG
Notes:	The probe template was PCR amplified from IMAGE:3153977 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3153977 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001007 same experiment
	EMAGE:21542 same embryo
	EMAGE:21541 same embryo
	EMAGE:21544 same embryo
	EMAGE:21545 same embryo
	EMAGE:21543 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source