EMAGE mouse gene expression spatial database

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EMAGE:21542

Tesc tescalcin ( MGI:1930803)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001016. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001016_01	euxassay_001016_02	euxassay_001016_03	euxassay_001016_04

euxassay_001016_05	euxassay_001016_06	euxassay_001016_07	euxassay_001016_08	euxassay_001016_09

euxassay_001016_10	euxassay_001016_11	euxassay_001016_12	euxassay_001016_13	euxassay_001016_14

euxassay_001016_15	euxassay_001016_16	euxassay_001016_17	euxassay_001016_18	euxassay_001016_19

euxassay_001016_20	euxassay_001016_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21542_wholemount_strong.wlz

21542_wholemount_moderate.wlz

21542_wholemount_weak.wlz

21542_wholemount_possible.wlz

21542_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21542_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

homogeneous

weak expression: see section 8 9 0 1 7 8 9

thalamus

moderate

regional

moderate expression: see section 1 3

cerebral cortex

moderate

regional

moderate expression: see section 0 1 3 4 5 weak expression: see section 5 6 7 8 9 6 7 8 9

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 7 8 9 0 1 2 4 5 6 weak expression: see section 7 8

testis

weak

regional

weak expression: see section 5 6 7 8 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T340
Entity Detected:	Tesc, tescalcin ( MGI:1930803)
Sequence:	sense strand is shown >T340 CGCGTCCCCACCCGGCTCCAGCGCAGCGCAGCCCAGGCAGTCCCCGGCCTGCGTGGGGCGCCCGGGGCCC CGCGGCGCACCATGGGCGCTGCCCACTCGGCGTCCGAGGAGGTGCGGGAGCTCGAGGGCAAGACCGGCTT CTCCTCGGACCAGATAGAGCAGCTGCATCGGAGGTTCAAGCAGCTAAGCGGGGACCAGCCCACCATTCGC AAGGAGAACTTCAACAATGTCCCTGACCTGGAGCTCAACCCGATCCGATCCAAAATCGTCCGTGCCTTCT TTGACAACAGGAACCTGCGAAAGGGATCCAGCGGTCTGGCCGATGAGATCAACTTTGAGGACTTCCTGAC TATCATGTCCTACTTCCGGCCCATCGACACCACCCTGGGCGAGGAACAGGTGGAGCTGTCACGAAAGGAG AAGCTGAAATTTCTGTTTCATATGTATGATTCGGACAGTGACGGCCGCATCACCCTGGAAGAGTATAGAA AT
Notes:	The probe template was PCR amplified from IMAGE:3154680 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3154680 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001016 same experiment
	EMAGE:21540 same embryo
	EMAGE:21541 same embryo
	EMAGE:21544 same embryo
	EMAGE:21545 same embryo
	EMAGE:21543 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source