EMAGE mouse gene expression spatial database

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EMAGE:21544

Papd7 PAP associated domain containing 7 ( MGI:2682295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001070_01	euxassay_001070_02	euxassay_001070_03	euxassay_001070_04	euxassay_001070_05

euxassay_001070_06	euxassay_001070_07	euxassay_001070_08	euxassay_001070_09	euxassay_001070_10

euxassay_001070_11	euxassay_001070_12	euxassay_001070_13	euxassay_001070_14	euxassay_001070_15

euxassay_001070_16	euxassay_001070_17	euxassay_001070_18	euxassay_001070_19	euxassay_001070_20

euxassay_001070_21	euxassay_001070_22	euxassay_001070_23	euxassay_001070_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21544_wholemount_strong.wlz

21544_wholemount_moderate.wlz

21544_wholemount_weak.wlz

21544_wholemount_possible.wlz

21544_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21544_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 7 8 9 0 1 9 0 1 2 weak expression: see section 8

vibrissa

weak

regional

weak expression: see section 7 8 9 2 3

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

lower jaw incisor

weak

regional

weak expression: see section 2 3 4

upper jaw incisor

weak

regional

weak expression: see section 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T277
Entity Detected:	Papd7, PAP associated domain containing 7 ( MGI:2682295)
Sequence:	sense strand is shown >T277 CCAGACCAGGGTTACTATCCCTCCACCAACCCTCGGAGTCGCCCCTGTTCCTTGCAGACAGGCTGGTGTC GACGGAACCACATCTTTGAAGGCTGTTCACAGTGTAACTTCCCCAGCCATTCCCTCGGCATCCCCCAACC CACTGTCTAGTCCTCATCTGTATCACAAGCAGCACAATGGCATGAAACTGTCCATGAAAGGCTCCCACAA CCACACTCAGGGTGGCGGCTACAGCTCTGTGGGCAGTGGAGCTGTGAGGCCTCCTGTAGGCAACCGAGGA CATCATCAGTACAACCGCACCGGCTGGAGGAGAAAAAAGCACGCACACACAAGGGACAGCCTGCCCGTGA GTCTCAGCAGATAATGGTCCTGACTGACTGCCCAAAGGCCTCGCTCGGGCACCACAGGGGAGCCGAGACC AGCATCCAGCACCTCCACCGCTGTCTGCCAAGCGCAGCCCAGCACTGGTCACTCTGCATGTTTGTGTGGT GGCCGCATCCATCTTACAGAACAGCTC
Notes:	The probe template was PCR amplified from IMAGE:2655369 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2655369 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001070 same experiment
	EMAGE:21540 same embryo
	EMAGE:21542 same embryo
	EMAGE:21541 same embryo
	EMAGE:21545 same embryo
	EMAGE:21543 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source