EMAGE mouse gene expression spatial database

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EMAGE:21546

Tmx1 thioredoxin-related transmembrane protein 1 ( MGI:1919986)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003033_01	euxassay_003033_02	euxassay_003033_03	euxassay_003033_04	euxassay_003033_05

euxassay_003033_06	euxassay_003033_07	euxassay_003033_08	euxassay_003033_09	euxassay_003033_10

euxassay_003033_11	euxassay_003033_12	euxassay_003033_13	euxassay_003033_14	euxassay_003033_15

euxassay_003033_16	euxassay_003033_17	euxassay_003033_18	euxassay_003033_19	euxassay_003033_20

euxassay_003033_21	euxassay_003033_22	euxassay_003033_23	euxassay_003033_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21546_wholemount_strong.wlz

21546_wholemount_moderate.wlz

21546_wholemount_weak.wlz

21546_wholemount_possible.wlz

21546_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21546_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4246
Entity Detected:	Tmx1, thioredoxin-related transmembrane protein 1 ( MGI:1919986)
Sequence:	sense strand is shown >T4246 GGGTGAGCGGCTGACATGGCGCACTTGGGGCGTCTTATGGTTCCCCTGGCAGCTCTGGTCCTGCTGCTCT GGGCGGTCCCTGGGGCCCACGGACGGCGGAATAACGTGCGCGTCCTCACCGATGAGAACTGGACGTCGTT GCTGGAAGGAGAGTGGATGATAGAATTTTATGCTCCCTGGTGTCCTGCTTGTCAGAATCTTCAGCCAGAA TGGGAAAGTTTTGCCGAGTGGGGAGAAGATCTTGAGGTGAAAGTTGCAAAAGTGGATGTCACAGAGCAGA CAGGACTAAGTGGACGGTTTATCATAACTGCTCTTCCTTCTATTTATCATTGTAAAGATGGTGAATTTAG GCGCTATGTGGGCCCAAGGACTAAGAAGGACTTCATAAACTTCGTAAGTGATAAAGAATGGAAGAATATT GAACCTATATCATCATGGTTTGGTCCAAGTTCTGTTCTGATGACTATGATGTCAGCACTTTTTCAACTCT CTGTGTACATCAGGACTAGCCACAGCTATTTTGTTCATGACCTTGGAATACCAGCGTGGGGTTCATACTT GGTTTTTGCTTTTGCCACAGTACTCTCAGGACTGCTGCTGGGACTTTGTATGATATTTGTAGCAGATTGC CTTTGCCCTTCAAAAAGACGCAAACCACAGCAACAGTATGCAAAAAAAACATCACCAGAATTTTCTCAAC CTCTGAAAAAAGTGGAGGAAGAGCAGGAGGCTGATGAAGAAGATGTTTCAGAAGAGGAGGCAGAAGATAG AGAGGGAGCAAGCAAAGCCACTTCTCAGAGCAGCATAAGACAACGCTGTGTGGGCCTCCCATCTGCCACG GACACATCCTAAGTTTTACATATATCTTAATACTATGACTTTGGCAAAACTGAAGCTGGGTGATCATTTC TATTGCGAGCTGAACTATGACTTGCTGTGTATACAGTGTAGGTCCTTTAGGTTGGACAGTTTCCATTCAG GAAATAAAAACAGACATAAGAGTTTGAAAATATGACTTAAGAATATTATGATGGTTTAAAGTTATTTAAT TTCAGAAGATCCTGGTGCCAAGCAGTAAGATTTGTGTTTATATTTAATAATCTATTTTGTGTGAGATTCG AAACTGCCTTTCCCAAGATTTGCATTACTGGAGTATTTAAGAAAATTACCTTAGAGAGAAGGATTTCTCA TTTCACATCATTTTTCTCAGTTTCACTGTGTGAAAAAGGATCATATTTCCCATAAGTGGGAACATTGCCC ATTATTTCAAGACATATACTTCATTGATAGTACTGCACTTAGGTAGAGTCCAAAATTTCCTCATAGATTT TGGATTATGCAGCTAACAAAACTACCTTACTTTATAGCTTTCATTTCTACACATTACATTTAGGCTATGT TGCTGACTTAAGAAGTTTTTATAAGTCTATATGGCATTGGATTAATTCTTAAGGCTGACGCCTATTTTGG TGTCTTGTTCACTGTATGATTTTTGTTATTTGCCATGTGTTACATTTCTAAGTTGGAAAAAATTACCAGT ATCACTGTTGTGTTTGCTATGGGTAAACAGAACTTTTCTATCCTTTTAGGATGAAGTTTACTGAGAGAAC CATCAAATCGAACTGTTTACTTGAAAATGTACAAATTTGGCTATACATTCCAGATTTTAAATTATTATTC TGAAGTTTTCTCTTTTTTAATGTGAAGATGAAATTTTCTGATTTTTAAAATTTGATGTGGAAAAGCTTTG GTATTTTATATTTTGGAAATTCAGAATTTAATTGATGTAGAATGAAGTCTGCATTCTACTCAAGAAACAT CTACTACTATGTGTTTTTAGTGTAATTCTGTCCTCCACGGGGTCTTGATGAATTTTATAATCAGGATATT GCTTTGTGTTTGAATATTGTAACACTATTGTGTTTTAAAGACAAATTATATATTGTATTGTTGTTGACTG CTAGTACTGTGTATCTCACAAATGGAAACAAGATCGGCAAAAGGACTACATGGATTTCATCTTACAAAGT GGGAAGGGTATCATGGGGGCAGGGACTGTCTTGATGTCTGGAGGGGGAAAGCAGACTGAATTCTTGATGA GTCAGTCACCAGCCTTTATGGAATAGATGGTGTCTGTGACACTCCCTCCCCTTATGTTCTCTGTGTGACT TCCTTAGACTTGCAGTACTGTGATGGGCAACAGCTTCTTTTAGGCCTCTCCTTTATAGTTTAAATGTTCT GCTGTCAGAGTTTGGATATGTAGTTTGTGGTGGCTTAGAAAAACATTCTAAGCACAAAATAAATGTTTCT AAGCACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503129 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503129 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003033 same experiment
	EMAGE:21550 same embryo
	EMAGE:21551 same embryo
	EMAGE:21547 same embryo
	EMAGE:21549 same embryo
	EMAGE:21548 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source