EMAGE mouse gene expression spatial database

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EMAGE:21547

Slc39a4 solute carrier family 39 (zinc transporter), member 4 ( MGI:1919277)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001118_01	euxassay_001118_02	euxassay_001118_03	euxassay_001118_04	euxassay_001118_05

euxassay_001118_06	euxassay_001118_07	euxassay_001118_08	euxassay_001118_09	euxassay_001118_10

euxassay_001118_11	euxassay_001118_12	euxassay_001118_13	euxassay_001118_14	euxassay_001118_15

euxassay_001118_16	euxassay_001118_17	euxassay_001118_18	euxassay_001118_19	euxassay_001118_20

euxassay_001118_21	euxassay_001118_22	euxassay_001118_23	euxassay_001118_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21547_wholemount_strong.wlz

21547_wholemount_moderate.wlz

21547_wholemount_weak.wlz

21547_wholemount_possible.wlz

21547_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21547_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw incisor

weak

regional

weak expression: see section 3 7 8

lower jaw molar

weak

regional

weak expression: see section 8 0 1

upper jaw incisor

weak

regional

weak expression: see section 2 3 7 8

upper jaw molar

weak

regional

weak expression: see section 8 0 1

liver lobe

weak

homogeneous

weak expression: see section 3 4 5 6 7 8 9 0 1 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4656
Entity Detected:	Slc39a4, solute carrier family 39 (zinc transporter), member 4 ( MGI:1919277)
Sequence:	sense strand is shown >T4656 CTCCAGTCTGGCCCCGGACAGTCCCAGCTGTCTCCAGCTAGCCCAGAAGTCAGCACCTCTACAAGGAACG CTTTTGGGGGCCTGAGGCGTGATGCTCCCAAAGTCGGTCACACAGGGACTTGTGTTGGCTCTGCTGGTGG GCACAGTGGCAGTGGCCCGGCCCAGGAACCTGCTCAGCCTGCTCGCCTTGGGCCAGGGTGCTCTGGATCG CCTGGAACTGGACGGCCTGTTAAATACGCTGGTGGCCCGTGTGCACTGCACCGACGGGCCGTGTGAAAAG TGTCTGTCTGTGGAGAATGTCTTGGCTCTAGGCAAACCTGACAAGCCACAGCCTGCCCCAGAATCAGTCC TGGAGTCCAGACACATTATTTACCTTAGTGCTGCTGCTGCCCTCTACCTTAACAACCCAGAGAAAACATG CAAGGACATCCAAGCTGGCCTCTTGGCCTCCCATGTGGACGATTACCTGGCCACACTGGAGAGTCCAGAG GCCATGACCCTGGGTCTGAGCCAGCTACTGCAGAAGATTGAGGCCCATGCTGCCAGCCAACCCACCGGGG AGAAGACCTGTGTAGATCTTCCCCAACTGCTGGAGGAGGCTGAGGCAGCAGGGGTTTCCAAAAGCGCCGG CCTGGTCTTGACTGCCTTGCTGGATCATGTCATTAATGGGTCCTGCTTCCAAGGCCTGCCTAGCCCTCAG TACTTTGTGGACTTTGTGTTCAGGCTACACAGTAGTGACCCTCCCAATATCACGCTGCATGAACTGGAGA ATTTGATGCATCACCTTGGGGTGGGTGGAGAGGACCACAGTGACCATGATGACCACGGTGATCATGCTGA CCACAGTCATCCGGACAGGAAAGCCAGCCACCAAGACTCTGAGCTCCATACTCCCCACAACAGCAACTCT AGTGTATGGGACACGCTGTGCCTGAGTGCCAAAGATATAATGGCTGTGTATGGGCTATCTGAAGAGGCTG GGGTGAGCCCTCAGGCCTGGGCCCAACTGACCCCTGCCTTGGTCCAGCAGCAGCTAAGTGGAGCCTGCAG CCCCTACCCCACTATCCGTATTCAGGACCAGCTCAGTCAAACAGAGAGGTATCTCTATGGCTCGCTGGCC ACCCTGCTCATCTGCCTCTGTGCTGTGTTCGGTCTTCTGCTGCTGACCTGTGCCAAATGCAGCACAGCCA CCCACTACATCATGCAGACCTTCCTAAGCTTGGCTGTGGGCGCACTTACCGGCGATGCTCTTCTGCACCT GATACCCAAGGTGCTGGGACTGCACACACATGGTGGAGAGGGTCACACCCATGAGGAGGAGGTGGGCGTT GGTGGGCAGGCCACCTGGCGCCTGCTGGCTGTACTTGGAGGCTTCTACATCTTCTTCCTGTTTGAGAGCT TCTTCAACCTCTTGTTGCCCAGGGACCAGGATTCTGAGAAAGATGGGCCTTGTAGCCATGGTGGGCACAG CCATGGAATATCTCTGCAGCTGGCACCAAGCAATCTCCGACAGTCCAAACAGACCCATGAAAGCTCTCGT TCAGACTTGGTGGCAGAGGAGACCCCGGAACTACTGAACCCAGAGACCCGGCGACTGAGAGCAGAGCTGA GACTGTTGCCCTATCTGATCACACTGGGCGACGCGGTACACAACTTCGCTGACGGGCTCGCTGTGGGCGC CGCCTTCTCATCCTCGTGGAAGACTGGGCTGGCCACTTCATTGGCGGTGTTCTGTCATGAGCTGCCCCAT GAACTCGGGGACTTCGCTGCTCTGCTGCATGCCGGGCTGAGTGTGAAGCGTGCGCTTTTGCTGAATCTGG CCTCAGCGCTCACAGCATTCGCAGGCCTCTACGTGGCTCTAGCAGTCGGAGTAGGCGAGGAGGGCGAGGC TTGGATTCTGGCGGTAGCAACCGGCCTCTTCCTTTACGTGGCGCTTTGTGACATGCTCCCAGCCATGATG AATGTGCGCGACCAGCGGCCCTGGCTTCTTTTCCTGCTCCACAACGTGGGTCTGCTGGGCGGCTGGACCG TCCTGCTGCTGCTGTCATTGTACGAAGACAACATCACCTTCTGACAGCTCTATCCCATCCCAGTCCTTGT CCCTGTCTGTTGACTCTGCTTTACCTTCTTAAGCCACCTAATTGTTGGCCCCACTACGGGTAGCCAGAGG CTTTGAGCCTCATTTCCTTGCTCTGACTTCAATAAAGACTTTTCAATCAAACCCAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5011088 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5011088 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001118 same experiment
	EMAGE:21550 same embryo
	EMAGE:21551 same embryo
	EMAGE:21549 same embryo
	EMAGE:21548 same embryo
	EMAGE:21546 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source