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EMAGE:21548

Rpl18a ribosomal protein L18A ( MGI:1924058)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21548Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21548_wholemount_strong.wlz
21548_wholemount_moderate.wlz
21548_wholemount_weak.wlz
21548_wholemount_possible.wlz
21548_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21548_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4658
Entity Detected:Rpl18a, ribosomal protein L18A ( MGI:1924058)
Sequence:sense strand is shown

>T4658
CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGTGAACGAGTG
CGCAGTGCCATGAAGGCCTCGGGCACGCTTCGGGAGTACAAGGTGGTGGGGCGCTGCTTGCCAACCCCAA
AATGCCACACACCACCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTCCCGCTT
CTGGTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCATCCGGGGAAATTGTGTACTGCGGACAGGTG
TTTGAGAAGTCACCCCTGCGTGTGAAGAACTTTGGCATCTGGCTGCGCTATGACTCCCGCAGTGGCACAC
ACAACATGTATCGGGAGTACAGGGACCTGACCACTGCTGGTGCAGTCACACAGTGCTACCGAGACATGGG
TGCCCGACACCGTGCCCGTGCCCACTCCATCCAGATCATGAAGGTGGAAGAGATTGCAGCTGGCAAGTGC
CGCCGGCCAGCTGTCAAGCAGTTCCACGACTCCAAGATCAAATTCCCACTGCCCCACCGTGTGTTGCGGC
GCCAGCACAAGCCACGCTTCACCACCAAGAGGCCCAACACCTTCTTCTAGACACAGAGACCCACTGAATA
AAAACTTGAGACTGTCAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5011713 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5011713 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003029 same experiment
 EMAGE:21550 same embryo
 EMAGE:21551 same embryo
 EMAGE:21547 same embryo
 EMAGE:21549 same embryo
 EMAGE:21546 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS