EMAGE mouse gene expression spatial database

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EMAGE:21551

Olfm1 olfactomedin 1 ( MGI:1860437)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001112_01	euxassay_001112_02	euxassay_001112_03	euxassay_001112_04	euxassay_001112_05

euxassay_001112_06	euxassay_001112_07	euxassay_001112_08	euxassay_001112_09	euxassay_001112_10

euxassay_001112_11	euxassay_001112_12	euxassay_001112_13	euxassay_001112_14	euxassay_001112_15

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21551_wholemount_strong.wlz

21551_wholemount_moderate.wlz

21551_wholemount_weak.wlz

21551_wholemount_possible.wlz

21551_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21551_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

regional

weak expression: see section 1 2 3 4 5 0 1 2 3 4 5

limb

weak

regional

weak expression: see section 1 2 4 5

vertebral axis musculature

weak

regional

weak expression: see section 1 2 3 4 4 5

facial vii ganglion

moderate

homogeneous

moderate expression: see section 2 3 4

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 3 1 2

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 1 2 3 0 1 2 3 4

vagus x ganglion

moderate

homogeneous

moderate expression: see section 4 0

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 2 3 0 1 2

dorsal root ganglion

moderate

regional

moderate expression: see section 3 4 5 6 7 0 1 2

lower jaw incisor

moderate

regional

moderate expression: see section 5 6 9

lower jaw molar

moderate

regional

moderate expression: see section 1 2

upper jaw incisor

moderate

regional

moderate expression: see section 6 9

upper jaw molar

moderate

regional

moderate expression: see section 3 8 1 2

axial skeleton

moderate

regional

moderate expression: see section 6 7 8 9 0

pectoral girdle and thoracic body wall skeleton

weak

regional

weak expression: see section 2 3 4 5 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4720
Entity Detected:	Olfm1, olfactomedin 1 ( MGI:1860437)
Sequence:	sense strand is shown >T4720 CTGCCCGGGGCGCGGGAGCCGGCGCCGGCGCGGCGCGGGCGCAGGAGGCCACTCGAGGCGCGATGTCGGT GCCGCTGCTGAAGATCGGGGTCGTGCTAAGCACCATGGCCATGATCACCAACTGGATGTCCCAGACGCTG CCCTCGCTGGTGGGCCTCAATACCACCCGGCTTTCGGCCGCCAGCGGCGGGACGCTGGACCGCAGCACCG GCGTGTTGCCCACCAACCCCGAGGAGAGCTGGCAGGTGTACAGCTCTGCCCAGGACAGTGAGGGCAGGTG TATCTGCACAGTGGTCGCCCCCCAGCAGACCATGTGTTCGCGGGATGCCCGCACAAAACAGCTGAGGCAG CTACTGGAGAAGGTGCAAAACATGTCTCAATCCATAGAAGTCTTGGACAGGCGAACTCAGAGAGACCTGC AGTACGTGGAGAAGATGGAGAACCAGATGAAAGGCCTGGAGACCAAGTTCAAGCAGGTGGAGGAGAGCCA TAAGCAGCATCTAGCCAGGCAGTTCAAGGCAATAAAAGCGAAAATGGATGAACTTAGGCCTTTGATTCCT GTGTTGGAAGAGTACAAGGCCGATGCCAAATTGGTATTGCAGTTTAAGGAGGAGGTCCAGAATCTGACGT CAGTGCTTAACGAACTGCAAGAGGAGATTGGCGCCTATGACTACGATGAACTTCAGAGCAGAGTGTCCAA TCTTGAAGAAAGGCTCCGTGCATGCATGCAAAAACTAGCCTGCGGGAAGCTGACAGGCATAAGTGACCCA GTGACTGTCAAGACCTCTGGCTCAAGGTTCGGGTCCTGGATGACAGACCCGCTGGCCCCAGAAGGCGATA ACCGGGTCTGGTACATGGATGGTTATCACAACAACCGCTTTGTCCGTGAGTACAAGTCCATGGTGGACTT CATGAACACGGACAACTTTACCTCTCACCGTCTCCCACACCCCTGGTCAGGTACGGGCCAGGTGGTCTAC AATGGCTCCATCTATTTCAACAAATTCCAGAGTCACATTATCATTAGGTTTGACCTGAAGACAGAGACGA TTCTGAAGACGCGCAGCCTGGACTATGCTGGCTACAACAATATGTATCACTACGCCTGGGGCGGCCACTC AGACATCGACCTCATGGTGGACGAGAATGGGCTCTGGGCTGTCTATGCCACAAACCAGAACGCAGGCAAC ATTGTCATCAGCAAGCTGGATCCTGTCTCACTGCAGATCCTCCAGACCTGGAACACCAGCTACCCCAAGC GCAGTGCCGGTGAGGCCTTCATCATCTGCGGCACCCTCTATGTCACCAACGGTTACTCGGGGGGCACCAA GGTCCACTATGCCTACCAAACCAACGCCTCGACCTACGAGTACATTGACATCCCCTTCCAGAACAAATAC TCTCACATCTCCATGCTGGACTACAACCCTAAAGACCGCGCGCTGTACGCCTGGAACAATGGCCATCAGA CCCTCTATAATGTCACCCTCTTCCATGTCATCCGCTCCGATGAGTTGTAGCTCCCGCTTTTCCAGAAGCC AAGAGTCCAAGTCCTCACCCGAGGACATTTCCATGAAACAGCTGCCAAATGATTCCAAGAATACTAACAG TCCTGATTTTGAGAAATCAGCGCAATTTTGACGCCGAGAGATGGTGTCCTCTCTGTGTTCCTCCTACTCC TGCAGGTGGGCCGCAGATTTCAGAAGAAACCGCCATGTATGTGGATGGAACTGCAGCCCAAGGCTCCTTG GCTGTCCCCATCCCACCCCATCCCTCTCTGGTCCAATAACTCAAAGAAGCAAGGCGATGACTGTTGGCCC CTCTCACCCAAGAACAACCCACTGTTAGGATTGCATGGACATTTCCTTAGGTTGTGCCCAGCGAGATGAT GTGATGGCCATTCTCCCCAACCAGCTCCCCCAGCCCCCGAGGCGCTCGCTCAGTGTAGGCTGCTTCAGCC CACACATATCGCAGCTGCCTTTTTCTTGACCGTGTTGTTGTCTCTTAGATTAACTGTGCTGAGACTCCCG GTAGCTCATGGACCCGTGTCTAGTACTTTCCCGAAGCGATGGTGGGTGTGCGTAGGTGAAGTTGCTGTAC CCACGTTGTTGAACTTGCGTACCCTGTAGCTAGATCGTGAGGTGTCCGTCTGTTATCCCCCAAGGTGGTA ACTCCGTGTGTTCAATTTATGCAATGAATGTTGTCATGCAATGCTGTAGTTTGGATTGATAAGTGGATGG TTTCTGTTTCTAAAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5036675 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5036675 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001112 same experiment
	EMAGE:21550 same embryo
	EMAGE:21547 same embryo
	EMAGE:21549 same embryo
	EMAGE:21548 same embryo
	EMAGE:21546 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source