EMAGE mouse gene expression spatial database

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EMAGE:21553

Enpp4 ectonucleotide pyrophosphatase/phosphodiesterase 4 ( MGI:2682634)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001105. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001105_01	euxassay_001105_02	euxassay_001105_03	euxassay_001105_04

euxassay_001105_05	euxassay_001105_06	euxassay_001105_07	euxassay_001105_08	euxassay_001105_09

euxassay_001105_10	euxassay_001105_11	euxassay_001105_12	euxassay_001105_13	euxassay_001105_14

euxassay_001105_15	euxassay_001105_16	euxassay_001105_17	euxassay_001105_18	euxassay_001105_19

euxassay_001105_20	euxassay_001105_21	euxassay_001105_22	euxassay_001105_23	euxassay_001105_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21553_wholemount_strong.wlz

21553_wholemount_moderate.wlz

21553_wholemount_weak.wlz

21553_wholemount_possible.wlz

21553_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21553_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

weak

regional

weak expression: see section 4 5 6 7 2 3

thymus primordium

moderate

homogeneous

moderate expression: see section 2 3 4 5 6

bladder

weak

regional

weak expression: see section 4 5

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4840
Entity Detected:	Enpp4, ectonucleotide pyrophosphatase/phosphodiesterase 4 ( MGI:2682634)
Sequence:	sense strand is shown >T4840 CCACGCGTCCGGCTGAGTTCCGCGCGCCAGACCGAAGTGGCGGTGGAGGCGGGTGAGTCCCGGAGGGAAA TGCCACTCCCTTCCCCAGCTCGCCGAGGGCGGAGGGTAGAGACGGGGCCCTAGCTGCTGTGTGCAACATG TTCAATATGAAGATATTAGTAATTCCTTTGTTTTGGGGACTTGTCACTGGCTATAAAGGGAACTCTTCCG ATAGCTCAGCACCTCGGTTACTTCTGGTATCCTTTGACGGCTTTAGAGCTGACTACCTGAAGAGCTATGA CCTTCCTCACCTCCAGAACTTTATCAAAGAAGGTGTCTTGGTGGAACATGTTAAAAATGTTTTTATCACC AAAACTTTTCCAAACCACTACAGCATTGTGACGGGCTTATATGAGGAAAACCATGGCATCGTGGCCAACT CCATGTATGACAGTGTCACAAAGAAACATTTTTCTGAATCCAATGATAAGGATCCGTTTTGGTGGAATGG AGCGGAGCCTATTTGGGTGACCAATCAGCTTCAGGAAAACAGGTCAAGCGCTGCCGCTATGTGGCCTGGC ACTGATGTGCCCATTCACAATATCACAGCTTCCTATTTTATGAACTATAGCAGCTCCGTGTCCTTTAAGG AAAGACTGGGTAACGTTACCACATGGCTCAGCAGTTCCAACCCACCAGTCACCTTTGCAGCGCTCTATTG GGAAGAACCAGATGTAAGCGGCCACAAGTATGGGCCTGAAGATAAAGAAAACATGCGCCGGGTATTGAAA GAAGTAGATGACCTTATTGGTGATATTGTCCTAAAGCTCAAGGTGTTAGGACTATGGGACAGCCTTAATG TGATCATTACAAGCGATCATGGGATGGCTCAGTGTTCTAAGAACAGACTGATAGATTTGGATTCCTGCAT CGACCGTTCAAACTACAGTGTTATAGACTTGACCCCTGTGGCTGCGATTCTTCCCAAAATAAATGTAACA GAGGTTTATGACAAACTGAAACGCTGTAACCCTCACATGAATGTTTATCTCAAAGAAGCTATCCCTAACA GATTTTACTACCAACACAGCAGTCGAATTCAGCCCATTATTTTGGTTGCTGAGGAAGGCTGGACAATCAC CCTGAACAAGTCATCATTTAAATTAGGTGACCATGGCTATGATAATTCCCTGCCTAGTATGCACCCGTTT CTTGCTGCCCACGGACCTGCCTTTAGAAAAGGTTACAGGCAGAGCACCATCAACACTGTGGATATTTATC CAATGATGTGCCACATCCTGGGACTAAAACCACATCCCAATAATGGAACCCTCAGTCATACCAAGTGCTT GTTGGTGGACCAGTGGTGCATTAATCTCCCAGAAGCCATTGGAATTGTTGTTAGTGCACTATTGGTATTA ACCATGCTAACAGGCCTCATGATATTCATGCGGAGCAGAGCATCCACATCCCGTCCATTCTCCCGTCTTC AGCTGCAGGAAGATGACGATGACCCTTTAATTGATTAATTCACCATGTCTTATTAATTTCAAAACTAAGG ATCCATGCAAGGAGTGGTGTTACAGCCATAAAAAAGGTATATTTCAAAAGACAAGACACACATGGCAAAA TTACTCTGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGCCCTGTTTTGGTGTGTTGCCTGATGCAAATAAC CCTGATGGTATTTAATTGCTGGTAAATCAGTTCAAACCACTAATGTCCCTAGCAAGACTAGCCTTTGAGA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065563 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065563 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001105 same experiment
	EMAGE:21556 same embryo
	EMAGE:21557 same embryo
	EMAGE:21555 same embryo
	EMAGE:21554 same embryo
	EMAGE:21552 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source