EMAGE mouse gene expression spatial database

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EMAGE:21554

Sap30bp SAP30 binding protein ( MGI:1927479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003056_01	euxassay_003056_02	euxassay_003056_03	euxassay_003056_04	euxassay_003056_05

euxassay_003056_06	euxassay_003056_07	euxassay_003056_08	euxassay_003056_09	euxassay_003056_10

euxassay_003056_11	euxassay_003056_12	euxassay_003056_13	euxassay_003056_14	euxassay_003056_15

euxassay_003056_16	euxassay_003056_17	euxassay_003056_18	euxassay_003056_19	euxassay_003056_20

euxassay_003056_21	euxassay_003056_22	euxassay_003056_23	euxassay_003056_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21554_wholemount_strong.wlz

21554_wholemount_moderate.wlz

21554_wholemount_weak.wlz

21554_wholemount_possible.wlz

21554_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21554_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 7 8 9 0 1 7 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4839
Entity Detected:	Sap30bp, SAP30 binding protein ( MGI:1927479)
Sequence:	sense strand is shown >T4839 CGCGCTGTGGGAATAAGATGGCGGGGAAGAAAAATGTTTTGTCGTCTCTTGCAATTTATGCGGAATATTC AGACCCTGAATCTGATGGCGAAACTGGAGTCGACGCTGTAGGCGGAGCGACTGAGGAGAAAGGTGGTTTG GTATCTGACGCCTATGGAGAAGATGACTTCTCTCGCCCAGGGGGTGATGAGGATGGTTATGAAGAAGAGG AGGACGAGAACAGTAAGCAGTCGGAAGATGACGATTCAGAGACTGAAAAACCTGAGGCTGATGACCCAAA GGATAACACAGAAGCAGAGAAGCGGGATCCCCAGGAATTAGTGGCCTCCTTTTCTGAAAGGGTTCGCAAC ATGTCACCCGATGAAATCAAGATCCCACCAGAACCGCCTGGCAGATGTTCAAATCACCTACAAGATAAGA TCCAGAAGCTCTACGAGCGGAAGATAAAGGAGGGGATGGATATGAACTACATCATCCAGAGGAAGAAGGA GTTCCGCAACCCCAGCATCTACGAGAAACTGATCCAGTTCTGTGCCATTGATGAGCTGGGCACCAACTAC CCAAAGGATATGTTCGATCCCCACGGCTGGTCTGAAGACTCCTACTATGAGGCATTAGCCAAGGCCCAGA AGATTGAAATGGACAAATTAGAAAAGGCCAAAAAGGAGCGAACCAAAATTGAGTTTGTAACGGGCACCAA GAAAGGCACCACGACCAATGCCACGGCCACCAGCACCAGTACTGCCAGCACAGCTGTCGCAGATGCCCAG AAGAGGAAGAGCAAGTGGGACTCTGCCATCCCCGTGACGACAATAGCACAGCCCACCATCCTCACCACCA CAGCCACCCTGCCAGCAGTGGTCACTGTCACCACTAGTGCCAGTGGCTCCAAGACCACCGTCATCTCTGC TGTGGGCACCATCGTGAAGAAGGCCAAGCAGTGACCAGAGGGTGCCCCAGGACTGGCAGTGACCAGAGGA CACCCCAGGCCTGGCAGGACTACAGTCCAGTAGCTTCCACCCACTGGGAGGTGCCACTCTGTGTGTTTAG GACTGAGATGCACTCCCCACATGCCTTCCAAGAGGAGCCTCTGTGACATTCCAGCCGGAAGGACATTACA GCAGAGGCAATGGTGTGCACTGGACAGGGTTTGTCCAGACACCTCTTGGGTTTCACCCAATTAAAAGTAC TGCTTCTTGGATCCTGGCATGTCAGGTGGACTGGCCTCTCCCATGCTGTGTTCGGGCAAACCTGAAGTTC AGGTGCCTCTCGCGACAGTCAAGATGTTGCTCAGGATGAACAGTGCTTGACAGGCCATAGGGGTGCTCAG AGGGGCAGCCCAGGGAGCAACCGTGCTCATTCCCAGTGTATGCCCAGGGCATGGGCAGGTGGGAGCTTAA GGAGGGGCCAGACACATTCTTTCCTGTTGTCTGTTTTCCTATTTTCCTGATCCTCAACAATTCACAACAA TTCTCAGGCAATAGCAGAGGCCGCTGCTCTCTTGGGTGCAGTTAAACACTCCCTACAGTGCTTCTGATAC ACACCCAGGCTTTTGAAATCAGGCCAGATAACAGAGGGCTGGGATGTGATTCCCAACCTGCCCAGGGCAG GAGCAAAGTGTGGGCACCTGGCCAAGGCCGCCCCAGCCCCTCAGTGCCCAGTGAGTGTGGCCAGCAGGAT CCACGCAGTGGTAGAGATGAATCACCTCCTGCTCTGGCCAGGGCAGCGGCTCATTGTTTATGTAAAGGCT GTGCTCGTGGGAAGGTCTCTGCCACCCCACCCTTCCTATGTCGTTATCTCTGCCCAGCAGCAGCCCCTGG GTAGCACCAGTGGCCACTCTTAGGGCCAGGATCCACCAGCCCAGCGCCAGACAGTGGGAAGACTTGTCTT TTGTGCCCCCTGGCAGCTCTCCTTCACACTCAGCGGAAGGGCTGAGTGTCAAAAGAGGAAGTGGGGCTGT GAAGGGTCCCCATGCTTCCGTGGGACCAACAGAGACACAGCCTCTGACGTTGTGTTTGGTATTTGTGTGA CCTGGGGACTGACACGCTGCCCATGAAATCTCTAAAATGAAAACTCAGTCACTGTTAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065414 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065414 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003056 same experiment
	EMAGE:21553 same embryo
	EMAGE:21556 same embryo
	EMAGE:21557 same embryo
	EMAGE:21555 same embryo
	EMAGE:21552 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source