EMAGE mouse gene expression spatial database

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EMAGE:21556

Thbd thrombomodulin ( MGI:98736)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001120_01	euxassay_001120_02	euxassay_001120_03	euxassay_001120_04	euxassay_001120_05

euxassay_001120_06	euxassay_001120_07	euxassay_001120_08	euxassay_001120_09	euxassay_001120_10

euxassay_001120_11	euxassay_001120_12	euxassay_001120_13	euxassay_001120_14	euxassay_001120_15

euxassay_001120_16	euxassay_001120_17	euxassay_001120_18	euxassay_001120_19	euxassay_001120_20

euxassay_001120_21	euxassay_001120_22	euxassay_001120_23	euxassay_001120_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21556_wholemount_strong.wlz

21556_wholemount_moderate.wlz

21556_wholemount_weak.wlz

21556_wholemount_possible.wlz

21556_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21556_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8 9 0 1

vibrissa

moderate

regional

moderate expression: see section 5 6 7 1 2 3

tongue

moderate

regional

moderate expression: see section 6 weak expression: see section 1 2 3 4 5

intermolar eminence

moderate

regional

moderate expression: see section 6

stomach

weak

homogeneous

weak expression: see section 3 4 5 6 7 8 9 0

hindgut

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8

midgut

moderate

regional

moderate expression: see section 9 0 1 2 3 4 5 6 7 8 9

lower jaw incisor

moderate

regional

moderate expression: see section 0 1 2 4 6 7 8

lower jaw molar

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 8 9 0 1 2 3 4

upper jaw incisor

moderate

regional

moderate expression: see section 0 1 2 7 8

upper jaw molar

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 8 9 0 1 2 3 4

genital tubercle of female

moderate

regional

moderate expression: see section 4 5 6 7

lung

moderate

homogeneous

moderate expression: see section 5 6 7 8 9 0 1 2 5 6 7 8 9 0 1 2 3 4

skeleton

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

chondrocranium

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 4 5 6 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4837
Entity Detected:	Thbd, thrombomodulin ( MGI:98736)
Sequence:	sense strand is shown >T4837 CCACGCGTCCGGCAGGGACTAGGAGCCTGGGCTAGAAACGTCTAGGTTGTGATAGAGGCTAGCTGCTGTC GCCGCAGGCATACGGAAACCCCTCTGCCTGGACTGGGTGTCTGCACCAGCGCAGGGCTAGCTTTTCTCGG AACTTGCACGGTGCACCCCTGGACAGCATGCTTGGGATTTTCTTTCTGGGTGTGCTGGCTCCAGCTAGCC TGGGGCTCTCCGCACTAGCCAAGCTGCAGCCCACAGGCAGTCAATGCGTGGAGCATGAGTGCTTCGCGCT TTTCCAGGGCCCCGCGACCTTCCTCGATGCCAGCCAGGCCTGCCAGCGCCTGCAAGGACATTTGATGACA GTGCGCTCCTCAGTGGCTGCCGATGTCATCTCCCTTCTGCTGAGCCAGAGCAGTATGGATTTAGGGCCCT GGATCGGTTTACAGCTCCCGCAGGGCTGTGACGACCCGGTGCATCTCGGGCCCCTGCGCGGCTTCCAGTG GGTTACTGGCGATAACCACACCAGTTACAGCAGGTGGGCGCGGCCCAACGACCAGACGGCTCCACTCTGC GGCCCTCTGTGCGTCACGGTCTCGACAGCAACTGAAGCTGCACCCGGCGAGCCGGCCTGGGAAGAGAAGC CATGCGAGACTGAGACCCAGGGTTTCCTCTGTGAGTTTTACTTCACAGCTTCCTGCAGGCCTCTGACGGT GAATACTCGCGATCCTGAGGCTGCCCACATCTCTAGTACCTACAACACCCCGTTCGGGGTCAGTGGTGCG GACTTTCAAACGCTGCCGGTAGGCAGTTCCGCCGCGGTGGAGCCCCTTGGCTTGGAGCTGGTGTGCAGGG CCCCGCCCGGAACTTCAGAGGGACACTGGGCTTGGGAAGCGACAGGAGCCTGGAATTGCAGCGTGGAGAA TGGTGGCTGTGAGTACTTGTGCAATAGGAGCACGAATGAACCCAGATGCCTCTGCCCCAGAGACATGGAC CTGCAGGCCGATGGACGTTCGTGTGCAAGACCTGTGGTTCAATCGTGCAACGAACTCTGCGAGCATTTTT GTGTCAGCAACGCTGAAGTGCCAGGCTCTTACTCCTGTATGTGTGAGACAGGCTACCAGTTGGCTGCAGA CGGACACCGGTGTGAGGACGTGGATGACTGTAAGCAGGGGCCCAATCCATGTCCCCAGCTCTGTGTTAAC ACCAAGGGCGGCTTCGAATGCTTCTGCTATGATGGCTATGAGTTGGTGGATGGAGAGTGCGTGGAGCTTC TGGATCCGTGTTTCGGATCTAACTGCGAGTTTCAGTGCCAGCCAGTGAGCCCCACCGACTACCGATGCAT CTGCGCTCCGGGCTTCGCACCCAAGCCGGATGAACCGCACAAGTGCGAAATGTTCTGCAATGAAACTTCG TGCCCAGCAGACTGTGACCCTAACTCTCCTACTGTTTGTGAATGCCCTGAAGGCTTCATCCTGGACGAGG GTTCCGTATGCACGGACATTGATGAGTGCAGTCAAGGTGAATGCTTCACCAGTGAATGTCGAAACTTCCC TGGCTCCTATGAGTGTATCTGCGGGCCTGACACAGCCCTTGCTGGTCAGATTAGCAAAGACTGTGACCCC ATCCCTGTTAGGGAAGACACCAAGGAAGAGGAGGGCTCTGGGGAGCCTCCAGTCAGCCCTACGCCAGGCT CTCCGACAGGTCCCCCTTCTGCAAGGCCAGTGCACTCTGGCGTGCTCATTGGCATCTCCATTGCCAGCCT GTCCCTGGTGGTGGCGCTTTTGGCGCTTCTCTGTCACCTGCGCAAGAAGCAGGGCGCTGCTCGCGCAGAG CTGGAGTACAAGTGCGCATCTTCCGCCAAGGAGGTAGTGCTGCAGCACGTCAGGACTGATCGGACGCTGC AGAAGTTCTGAGGGATTTGCTCCAGAGACCCAGGTGGCCTTTGTCTTTCCGGGCTCTGTACCTCTCCTCT CCTCTCTCTCTCTTCAGCCTCCCAGCTGTGTTCTCTGGCAACTTAAAAGCATCCTGGCTGGTATAATAAC CAGAGAAGAGCCCATCCTCTCAGGACAAGCGAGGGTAGGGAGGACTGGAAGCAGGACAGCCCAGTTTCTT CCAAGTAGATACTGGACAACTGGGCGGAGGTGGCAAATACAGCGGAGATCCCAGAGTACCCCAGTCCCTC ACCTCACCTCCTAGTGCTGCTGATCTGTAGGCTTGAAGGCAAACCTTGACCCCATGGGCTGGAGATGACC CAGATATTTATTTTTTTTAAAAGTATTTAGTATTTTCTTCCCTCCAGTTTTCTTCTGCTTGTAAGTCTCC AGCCCCCCACCGCTTTCTCAGTCCCTCCATTCCCCCCTCCTGTCATTCTCCTCCCCAAACCTGATCATAA CTTTGCCCTTACCGTTGTTTCCAAACTCTTATGTGAAACAGAAAATAACACTAAAAGCAGAAAACGTTCT TTTTCACTGGCTTTGGGTATTTAGTCAGAAATTTCAGGTAACCAAAGCAAAAGAATTTTAACAAAAGCTA AAATATTTCAGCTGAACACTAACTAGTCAATAGTGCTGGAATGTCACAGAAAATAAACTTAAGGAAGTAG GGTTTTTTTTTTGAAATCTTTGTTTTTGAAAAGGGTGAGCCTGGGTTTTATGATTGTTGCTGTTGTTTTG AATGGGAATGACAAAAGAGGTCATTATTGTTAAGATTTTTATGCAGGCTCTACAGTGTTATTAATTTTTG ACAGTGTTCAAAATGTGCAGAGGATCCTTTGTCCAACCCTTTGACATGACAATAGGACATTGCTATCTTC AGACATACTGGGCCACATTCATAGCTTTCCAAGGATGTATGTGGTCCTGCCTCAACATATCAGAGACTGA CAGATGGAAGCATCTTCCAGTAAAGCATGAGTTGTGTGCTTCGTGCCGAGCTGACTCTCAACTGTGCTTG TCCCTTGTAGTCCCGAAATACAAGCAATGTGCTGCTTAGGGAAACATGGAAACTTGGGAATGGAGTCTGG GGGTGCCTAGATGGGGCTTTCTTTTAATGAGACTCTTGAACAATATCTCGTAATTCAGAGGGATCTTCTA GCCCTGGCCACTGGCCTGTACACAAGAATTGGGACCTCGCTTGGGATCTGGCTAGAATTGCAAAATCCTA GCCCCCACCCCTGCCCCACCCCAGTGTGCCAGTTCATAAGAATCTGCATTTTGACAACATCCACAGGGAC ATTGTCCAGTCATTTCAGGACAACTGGTCTTAAGAGTTTCCAACCTTTGTAGAACATTTAAATGTCGGTT AATAATAAGTAGCAGGCCATGTTAAGGCCATTTATTATCAAGAAACTGAGGAATTTTCTCTGCATAGCTT TGCTTTCTGGATACAATAAAATGAGAAGGTACACACCTCTAGATAGTGCCACACAGAGTCCAGAAGGGTT TTGTTTTAAGTAAGCTAGGAATGAGTTCATATGTTAGTGTAAGGAACAAATGTATTATATGTGTATCTTT TGTAAAGAAAGGTTTTTCTTTACGGTTTTGTAAGCTCAGCATATTTGTACATATTTATTTATTGGAGTTT CGCTAGAACACAAGCAAAGCCTTTGCTTATGACGTCACATGTACAAAATAAATAGATGACAGTGTAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5065481 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5065481 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001120 same experiment
	EMAGE:21553 same embryo
	EMAGE:21557 same embryo
	EMAGE:21555 same embryo
	EMAGE:21554 same embryo
	EMAGE:21552 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source