EMAGE mouse gene expression spatial database

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EMAGE:21564

Smyd4 SET and MYND domain containing 4 ( MGI:2442796)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003031_01	euxassay_003031_02	euxassay_003031_03	euxassay_003031_04	euxassay_003031_05

euxassay_003031_06	euxassay_003031_07	euxassay_003031_08	euxassay_003031_09	euxassay_003031_10

euxassay_003031_11	euxassay_003031_12	euxassay_003031_13	euxassay_003031_14	euxassay_003031_15

euxassay_003031_16	euxassay_003031_17	euxassay_003031_18	euxassay_003031_19	euxassay_003031_20

euxassay_003031_21	euxassay_003031_22	euxassay_003031_23	euxassay_003031_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21564_wholemount_strong.wlz

21564_wholemount_moderate.wlz

21564_wholemount_weak.wlz

21564_wholemount_possible.wlz

21564_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21564_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4727
Entity Detected:	Smyd4, SET and MYND domain containing 4 ( MGI:2442796)
Sequence:	sense strand is shown >T4727 GCGCGGTGCTACTGAGTTGTTCCCCCGCCAGCTGTCGGAACTTTGCCCGCCCAGTCCTTTGGCGGATAGA CAGAATGGCAACCCTGGGAACAATCGGAGCTCTCCCCTGGTAACTGCTGCTAAATATAGTCAAAGCAGTG ACCTGGGTACTTCTTCACGCAGTGCGTGCCCGGCGCCGGTGCCAGGCCCAGAGCTTGGCACTGTGGGATA AACAAGAGCTGCGGCAGCATGGATCTACCGGTGGATGAATGGAAGTCCTACCTACTTAAGAAGTGGGCTT CACTCCCGAAGTCTGTGCAGGACACAATTTCTACAGCAGAGACTTTGAGCGACATCTTCCTTCCTTCTTC TTCCCTTCTTCAACCTGAGGATGAGATGTTTTTGAAGGAACTTTCTAGTAGTTACTCGGTGGAAAAGGAC TATGATGCTCCCCTCTTCTACAGAGAAGAAGGAAACAGGAAATTCCAAGAAAAGGAGTACACAGATGCTG CAGTGCTGTACTCTAAGGGAGTGTCTCATTCAAGGCCTAACACTGAGGACATTTCACTGTGCTATGCCAA TCGCTCTGCAGCGCTCTTCCATCTGGGTCAGTATGAAGCATGTCTTAAAGACATAGTGGAAGCAGGTATG CATGGGTATCCTGAAAGACTGCAGCCCAAGATGATGGTGCGTAAGACAGAATGCCTGGTGAACCTGGGGA GACTCCAGGAGGCAAGACAGACCATCAGTGATCTCGAAAGCAGCCTCACTGCCAAGCCAACCCTGGTGCT TTCCTCTTACCAGATTCTGCAAAGGAATGTCCAGCATCTGAAAATAAAGATCCAAGAAAAGGAGACTCTC CCAGAACCCGTCCCTGCAGCTCTCACCAATGCCTTTGAGGATATAGCCCTGGGGGAAGAGAACAAACAGA TTTCTGGGGCCTCCCTCTCTGTCAGCTTATGCACACACCCTTTGAAAGGCCGCCATCTAGTTGCCACAAA AGACATTCTCCCAGGAGAACTGCTGGTGAAGGAAGATGCTTTTGTAAGTGTCCTTATCCCAGGAGAAATG CCACGACCTCATCATTGCCTTGAGAACAAGTGGGATACCAGAGTTACCAGTGGAGACCTCTACTGTCACC GATGTCTGAAGCACACTTTGGCCACAGTACCTTGTGGCAGCTGCAGCTATGCCAAGTATTGCAGCCAGGA ATGTATGCAGCAGGCATGGGACCTCTACCATAGCACAGAGTGTTCTCTTGGGGGGCTGCTCCTCACACTC GGGGTCTTCTGCCATGTTGCCCTGAGAATGACTCTTTTAGCCAGATTTGAAGATGTTGATAGAGTTGTAA GGATGCTTTGTGACGAGGTTGGTAGCACAGACACCTGTTTACCTGAAAGCAAGAATCTGGTCAAGGCATT TGATTACACAAGTCAGGAAGAGAGTGAAGAGAAGAGCAAGATAGGTGAACCCCCAATTCCTGGATGCAAT GTCAATGGAAAGTATGGAAGTAATTATAATGCTATCTTCAGCCTTTTGCCCCATACTGAAAAGCATAGCC CAGAACACAGATTCATCTGTGCCATCAGTGTCTCCGCACTGTGCAGACAACTCAAAGCCGCCAGCGTGCA GGCCCAAACCTTAAAGTCCCCTAAGCTGAAAGCAGTGACCCCAGGGCTGTGTGCAGATTTGACTGTTTGG GGAACAGCCATGCTGCGACACATGCTACAGCTGCAGTGTAATGCCCAGGCAATAACATCCATATGTCACA CAGGGTCTAACGAGAGCATCATTACCAACAGCAGGCAGATACGCCTTGCCACAGGCGTCTTCCCTGTTGT CAGCCTCCTGAACCACTCCTGCAGGCCCAACACCAGTGTGTCCTTCACTGGCACTGTCGCCACCATCCGG GCAGCACAGAGGATCGCAAAAGGACAGGAGATTCTGCACTGCTATGGGCCTCACGAGAGCCGGATGGGCG TTGCTGAGAGGCAGCAGAGGCTGAGTTCTCAGTACTTCTTTGACTGCCGCTGTGGGGCCTGTCACGCTGA GACACTGAGAGCAGCTGCAGCTCCCAGATGGGAAGCCTTCTGTTGTAAGACTTGCAGAGCGCTCATGCAG GGAAATGATGTGCTGAGCTGTAGCAACGAATCTTGCACAAACTCTGTCAGCAGGGACCAGCTAGTCTCTC GCCTGCAGGACCTTCAGCAACAGGTCTGCATGGCCCAGAAGCTTCTCCGAACCGGTAAACCAGAGCAAGC CATTCAGCAGTTGTTGCGGTGCCGGGAGGCTGCTGAGAGCTTCTTGTCAGCAGAGCACACCGTACTGGGG GAAATTGAAGACGGCCTGGCCCAGGCCCATGCTACCTTAGGGAACTGGCTAAAGTCTGCTGCCCATGTAC AGAAGAGTCTCCAGGTGGTTGAAACTCGTCATGGGCCATCCAGTGTTGAAATTGGCCATGAGCTCTTCAA ACTGGCCCAAGTCCTGTTCAATGGGTTGGCAGTGCCTGAAGCTCTGAGTGCCATCTGGAAGGCAGAAAGG ATCCTGTTGGTGCACTGTGGCCCTGAGAGTGAGGAGGTCCGGGAGCTCCGGGAAATGAGGTCCTGCTTAC TGGACTCGTCATTCGTCCCTGTGGGGCCCTTGGTGTAGAGCATGCGTCCTCACCCTCAAGAAGGAGCTCT GGTGATGACTGAGATGTTCTGTTGGCTTGGAGCTCTCAGAGAGGACGGGACCTTCCCACCTGACCTGAGC CTAGTGTCTGGCACAGGGAGCACTTGAAAACAGATTGAGACGCTCACCTGCCATGCTGGCTGCTGCTTGC AGGAGCTAACTGCCCTCTGATGGAAACCCCATGCCCAGAAAAGACTAAATCCAGTATCTAAAGGCTGCTT TAAAGGGTTGTCACTGCAGCCGGGCTTGGTGGCACACGCCTTTAATCCCAGCACTCGGGAGGCAGGCGGA TTTCTGAGTTCAAGGCCAGCTTGGTCTATAAAGTAAGTTCTAGGACAGCCAGGGCTACAGAGAAACCCTG TCTTGAAAAACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5037842 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5037842 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003031 same experiment
	EMAGE:21566 same embryo
	EMAGE:21568 same embryo
	EMAGE:21563 same embryo
	EMAGE:21565 same embryo
	EMAGE:21567 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source