EMAGE mouse gene expression spatial database

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EMAGE:21565

H2-T9 histocompatibility 2, T region locus 9 ( MGI:95965)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001107. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001107_01	euxassay_001107_02	euxassay_001107_03	euxassay_001107_04

euxassay_001107_05	euxassay_001107_06	euxassay_001107_07	euxassay_001107_08	euxassay_001107_09

euxassay_001107_10	euxassay_001107_11	euxassay_001107_12	euxassay_001107_13	euxassay_001107_14

euxassay_001107_15	euxassay_001107_16	euxassay_001107_17	euxassay_001107_18	euxassay_001107_19

euxassay_001107_20	euxassay_001107_21	euxassay_001107_22	euxassay_001107_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21565_wholemount_strong.wlz

21565_wholemount_moderate.wlz

21565_wholemount_weak.wlz

21565_wholemount_possible.wlz

21565_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21565_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 9 6 7 8 9 0 1 2 weak expression: see section 3 4 5 6 7 8

limb

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 0 1 2 3

vertebral axis musculature

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

foregut-midgut junction

moderate

homogeneous

moderate expression: see section 2 3 4 5 6 7 8 9 0 1

esophagus

moderate

regional

moderate expression: see section 3 4 5 8

tongue

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8

stomach

moderate

homogeneous

moderate expression: see section 3 4 5 6 7 8 9 0 1

hindgut

moderate

homogeneous

moderate expression: see section 3 4 5 6 7

midgut

moderate

homogeneous

moderate expression: see section 6 7 8 9 0 1 2 3 4 5 6 7 8 9

kidney calyx

weak

regional

weak expression: see section 0

kidney pelvis

weak

regional

weak expression: see section 9 0 1 2 8 9 1 2

lung

weak

spotted

weak expression: see section 6 7 8 9 0 1 2 7 8 9 0 1 2 3

chondrocranium

weak

regional

weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

tail

moderate

regional

moderate expression: see section 1 2 3 4 5 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4721
Entity Detected:	H2-T9, histocompatibility 2, T region locus 9 ( MGI:95965)
Sequence:	sense strand is shown >T4721 GGCAGGGTTGAGAGCAAGAAGGTGCAGAATGTAGACCTGCAGAATTATCAGACCCAGTGGCGGCTTCTGC CACTGGAAAAATTTCCAACATAAAACAGATGATCAGTTTGGGACAATCGATTCTGCGACCAGAGTTTATA AAGCTGTCCAAGATCTGCCCAGTCCCAGATGTCCTGGGTCCTCAGGGCGGCTGTGGTCTGCGCCCTTCTC CTGCAGCTGGATGCCAGACCATCCTGGACTCGGATCCCTTTGGGTTCACACTCGCTTAGGTATTTCTACA CCGCTGTGTCCCGGCCTGGCCTTGGGGAGCCCTGGTTCATAATCGTCGGCTATGTGGACGACATGCAGGT CCTGCGCTTCAGCAGCAAGGAGGAGACTCCGAGGATGGCACCCTGGCTGGAGCAGGAGGAAGCAGATGAC TGGGAACAGCAGACTCGTATAGTCACAATTCAAGGACAGCTGTCTGAAAGGAATCTGATGACCCTGGTTC ATTTTTACAACAAGAGCATGGACGACTCTCACACACTACAGTGGCTGCAAGGCTGCGATGTGGAGCCAGA TCGGCACCTGTGTCTCTGGTACAACCAGCTCGCCTATGATAGCGAGGATCTCCCCACCCTGAACGAAAAC CCAAGTTCCTGTACAGTGGGAAACAGCACTGTACCTCACATCTCTCAGGATCTGAAGAGCCACTGCTCAG ATCTGCTGCAGAAATACCTGGAAAAAGGGAAGGAGAGGCTGCTGCGTTCAGAACCCCCAAAGGCACATGT GACCCATCACCCCAGACCTGCAGGTGATGTCACCCTGAGGTGCTGGGCCCTGGGCTTCTACCCTGCTGAC ATCACCCTGACCTGGCAGAAGGATGGGGAGGAGCTGACCCAGGACATGGAGTTTGTAGAGACCAGGCCTG CAGGGGATGGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCTCTTGGGAAAGAGCAGAGTTACACATG CCATGTGTACCATGAGGGGCTGCCTGAGCCCCTCACCCTGAGATGGGAGCCTGCATGGTACCAAAAGCCT TGGATTTGGATTGTTGCCATGGTTTTCATTTTGCTCATCATTTGTCTCTGTGTGGTTTGCATATGCATGA AGAAGAATGCAGGTGGGAGAGGAAGGCGTGACACCCAAGAAGCAGGCAGAGACAGTCCCCAAGACTCTAG CAAGACTGTTGTGGATGATGAGGAGATGGGGGTTTGCTTTTGGAAGATTAAGTACTGTAAGACTTGTCTA GGCCACTCCCCAGGAACTTCAGTTGGCGAGTCTTTACTGTCACCTTGACTGGATTTAGGATCATCTGGGA GATGCCCCTTTGAGTGGCTGGGCTGTGAGGACAGCAGGCCAGTTCTTGCCACCCTGGACAGAAACACATC TCACCTTTCTGGCTCAAGGATCTGAACACCTGTCTCTTGCCTACTCGGCTTCTAGTCAGGCATTTTGTCA CCTTGTCAAGGGTCCCAGGGACACAAAGCTCCCTCCTCTCACCCACAGCACTCTGGGTCCTACCCTCAGT GCTTCAGGGACATTTAATCAGGTCAAATTGGGATCAATGGCTTTGATGCAGAAAAGAACTGTGGACTAAT AGAGATAGGGTTTAATTAAAAAATATATCTTTTTAATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5036676 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5036676 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001107 same experiment
	EMAGE:21566 same embryo
	EMAGE:21568 same embryo
	EMAGE:21563 same embryo
	EMAGE:21567 same embryo
	EMAGE:21564 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source